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Tailing and 3′-end labeling of RNA with yeast poly(A) polymerase and various nucleotides

Published online by Cambridge University Press:  01 February 1998

GEORGES MARTIN  and
WALTER KELLER
GEORGES MARTIN
Affiliation:
Department of Cell Biology, Biozentrum, University of Basel, Klingelbergstrasse 70, CH-4056 Basel, Switzerland
WALTER KELLER
Affiliation:
Department of Cell Biology, Biozentrum, University of Basel, Klingelbergstrasse 70, CH-4056 Basel, Switzerland
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Abstract

We have tested conditions for the labeling and tailing the 3′-end of RNAs with yeast poly(A) polymerase. Conditions were optimized for addition of NTP, dNTP, or ddNTP nucleotides to RNA. ATP, GTP, and UTP were useful for adding homopolymer tracts of various lengths. The nonradioactive nucleotides biotin-N6-ATP and digoxigenin-11-UTP also were used efficiently.

Keywords

biotin-N6-ATPdigoxigenin-11-UTPdigoxigenin-16-dATPfluorescein-12-ATPmonoaddition
Type
METHODS REPORT
Information
RNA , Volume 4 , Issue 2 , February 1998 , pp. 226 - 230
Copyright
© 1998 RNA Society

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