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Aspects of cellular aging

Published online by Cambridge University Press:  17 November 2008

Leonard Hayflick
Leonard Hayflick*
Affiliation:
University of California, San Francisco, Medical School, USA
*
Leonard Hayflick, University of California, San Francisco, Medical School, 36991 Greencroft Close, PO Box 89, The Sea Ranch, California 95497, USA.
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Abstract

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Type
Biological gerontology
Information
Reviews in Clinical Gerontology , Volume 3 , Issue 3 , August 1993 , pp. 207 - 222
Copyright
Copyright © Cambridge University Press 1993

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References

1Comfort, A.The biology of senescence. New York: Elsevier, 1979.Google Scholar
2Medawar, PB.The uniqueness of the individual. London: Methuen, 1957.Google Scholar
3Williams, GC.Pleiotropy, natural selection, and the evolution of senescence. Evolution 1957; 11: 398–411.CrossRefGoogle Scholar
4Kirkwood, TBL, Cremer, T.Cytogerontology since 1881: a reappraisal of August Weismann and a review of modern progress. Hum Genet 1982; 60: 101–21.CrossRefGoogle Scholar
5Weismann, A.Essays upon heredity and kindred biologicaiproblems, second edition. Oxford: Clarendon Press, 1891.Google Scholar
6Weismann, A.Aufsatze über vererbung und verwandte biologischefragen. Jena: Verlag von Gustav Fischer, 1892.Google Scholar
7Daniel, CW, deOme, KB, Young, JT, Blair, PB, Faulkin, LJ Jr. The in viva lifespan of normal and preneoplastic mouse mammary glands: a serial transplantation study. Proc Natl Acad Sci USA 1968; 61: 53–60.CrossRefGoogle Scholar
8Krohn, PL.Review lectures on senescence. II: heterochronic transplantation in the study of aging. Proc R Soc Lond (Biol) 1962; 157: 128–47.Google Scholar
9Cudkowicz, G, Upton, AC, Shearer, GM, Hughes, WL.Lymphocyte content and proliferatiave capacity of serially transplanted mouse bone marrow. Nature 1964; 201: 165–67.CrossRefGoogle ScholarPubMed
10Ford, CE, Micklem, HS, Gray, SM.Evidence of selective proliferation of reticular cell-clones in heavily irradiated mice. Br J Radiol 1959; 32: 280.Google Scholar
11Harrison, DE.Normal production of erythrocytes by mouse marrow continuous for 73 months. Proc Nail AcadSci USA 1973; 70: 3184–88.CrossRefGoogle ScholarPubMed
12Harrison, DE.Normal function of transplanted marrow cell lines from aged mice. J Gerontol 1975; 30: 279–85.CrossRefGoogle ScholarPubMed
13Heilman, S, Botnick, LE, Hannon, EC, Vigneulle, RM.Proliferative capacity of murine hematopoietic stem cells. Proc Natl Acad Sci USA 1978; 75: 490–94.CrossRefGoogle Scholar
14Siminovitch, L, Till, JE, Mcculloch, EA.Decline in colony-forming ability of marrow cells subjected to serial transplantation into irradiated mice. J Cell Compar Physiol 1964; 64: 23–31.CrossRefGoogle ScholarPubMed
15Hayflick, L. The coming of age of WI-38. In: Maramorosch, K ed. Advances in cell culture, volume three. Orlando: Academic Press, 1984: 303–16.Google Scholar
16Parker, RC.Methods of tissue culture. New York: Harper & Row, 1961.Google Scholar
17Kohler, G, Milstein, C.Continuous cultures of fused cells secreting antibody of predefined specificity. Nature 1975; 256: 495–97.CrossRefGoogle ScholarPubMed
18Hayflick, L, Moorhead, PS.The serial cultivation of human diploid cell strains. Exp Cell Res 1961; 25: 585–621.CrossRefGoogle ScholarPubMed
19Hayflick, L.The limited in vitro lifetime of human diploid cell strains. Exp Cell Res 1965; 37: 614–36.CrossRefGoogle ScholarPubMed
20Boone, CW.Malignant hemangioendotheliomas produced by subcutaneous inoculation of Balb/3T3 cells attached to glass beads. Science 1975; 188: 68–70.CrossRefGoogle ScholarPubMed
21Boone, CW, Takeichi, N, Paranjpe, M.Vasoformative sarcomas arising from Balb/3T3 cells attached to solid substrates. Cancer Res 1976; 36: 1626–33.Google ScholarPubMed
22Puck, TT, Cieciura, SJ, Fisher, HW.Clonal growth in vitro of human cells with fibroblastic morphology. J Exp Med 1957; 106: 145–58.Google Scholar
23Puck, TT, Cieciura, SJ, Robinson, A.Genetics of somatic mammalian cells. III. Long-term cultivation of euploid cells from human and animal subjects. J Exp Med 1958; 108: 945–56.Google Scholar
24Swim, HE, Parker, RF.Culture characteristics of human fibroblasts propagated serially. Am J Hygiene 1957; 66: 235–43.Google ScholarPubMed
25Ogura, H, Fujiwara, T, Namba, M.Establishment of two chick embryo fibroblastic cell lines. Gann 1984; 75: 410–14.Google ScholarPubMed
26Harris, M.Quantitative growth studies with chick myoblasts in glass substrate cultures. Growth 1957; 21: 149–66.Google ScholarPubMed
27Hay, J, Strehler, BL.The limited growth span of cell strains isolated from the chick embryo. Exp Gerontol 1967; 2: 123–35.CrossRefGoogle Scholar
28Lima, L, Macieira-Coetho, A.Parameters of aging in chicken embryo fibroblasts cultivated in vitro. Exp Cell Res 1972; 70: 279–84.CrossRefGoogle ScholarPubMed
29Witkowski, jA.Alexis Carrel and the mysticism of tissue culture. Med History 1979; 23: 279–96.CrossRefGoogle ScholarPubMed
30Witkowski, JA.Dr Carrel's immortal cells. Med History 1980; 24: 129–42.CrossRefGoogle ScholarPubMed
31Witkowski, JA, The myth of cell immortality. Trends Biochem Sci 1985; 10: 258–60.CrossRefGoogle Scholar
32Martin, GM, Sprague, CA, Epstein, CJ.Replicative lifespan of cultivated human cells. Effect on donor's age, tissue, and genotype. Lab Invest 1970; 23: 86–92.Google Scholar
33Goldstein, S, Moerman, EJ, Soeldner, JS, Gleason, RE, Barnett, DM.Chronologic and physiological age effect replicative lifespan of flbroblasts from diabetics, prediabetics, and normal donors. Science 1978; 199: 781–82.CrossRefGoogle Scholar
34Schneider, EL, Mitsui, Y.The relationship between in vitro cellular aging and in vivo human aging. Proc Natl Acad Sci USA 1976; 73: 3584–88.CrossRefGoogle Scholar
35Vracko, R, Mcfarland, BM.Lifespan of diabetic and non-diabetic fibroblasts in vitro. Exp Cell Res 1980; 129: 345–50.Google ScholarPubMed
36Leguilly, Y, Simon, M, Lenoir, P, Bourel, M.Long-term culture of human adult liver cells: morphological changes related to in vitro senescence and effect of donor's age on growth potential. Gerontologia 1973; 19: 303–13.CrossRefGoogle Scholar
37Bierman, EL.The effect of donor age on the in vitro lifespan of cultured human arterial smooth-muscle cells. In Vitro 1978; 14: 951–55.Google Scholar
38Tassin, J, Malaise, E, Courtois, Y.Human lens cells have an in vitro proliferative capacity inversely proportional to the donor age. Exp Cell Res 1979; 123: 388–92.CrossRefGoogle Scholar
39Lipman, RD, Taylor, A.The in vitro replicative potential and cellular morphology of human lens epithelial cells derived from different aged donors. Curr Eye Res 1987; 6: 1453–57.CrossRefGoogle ScholarPubMed
40Walford, RL.Studies in immunogerontology. J Am Geri air Soc 1982; 30: 617–25.CrossRefGoogle ScholarPubMed
41Walford, RL, Jawaid, SQ, Naeim, F.Evidence for in vitro senescence of T-lymphocytes cultured from normal human peripheral blood. Age 1981; 4: 67–70.CrossRefGoogle Scholar
42Hayflick, L.The biology of human aging. Am I Med Sci 1973; 265: 433–45.Google ScholarPubMed
43Rohme, D.Evidence for a relationship between longevity of mammalian species and life spans of normal fibroblasts in vitro and erythrocytes in vivo. Proc Natl Acad Sci USA 1981; 78: 5009–13.Google Scholar
44Stanley, JF, Pye, D, Macgregor, A.Comparison of doubling numbers attained by cultured animal cells with the life span of species. Nature 1975; 255: 158–59.Google Scholar
45Houck, JC, Sharma, VK, Hayflick, L.Functional failures of cultured human diploid fibroblasts after continued population doublings. Proc Soc Exp Biol Med 1971; 137: 331–33.CrossRefGoogle ScholarPubMed
46Hayffick, L. Cell aging. In: Eisdorfer, C ed. Annual review of gerontology and geriatrics. New York: Springer 1980: 26–67.Google Scholar
47Norwood, TH, Smith, JR, Stein, GH. Aging at the cellular level: the human fibroblastlike cell model. In: Schneider, EL, Rowe, JW eds. Handbook of the biology of aging. San Diego: Academic Press, 1990; 131–54.Google Scholar
48Hayflick, L.Aging under glass. Mutat Res 1991; 256: 69–80.Google Scholar
49Seshadri, T, Campisi, J.Repression of c-fos transcription and an altered genetic program in senescent human fibroblasts. Science 1990; 247: 205–209.CrossRefGoogle Scholar
50Lumpkin, CK Jr, Mcclung, JK, Pereira-Smith, OM, Smith, JR.Existence of high abundance antiproliferative mRNAs in senescent human diploid fibroblasts. Science 1986; 232: 393–95.CrossRefGoogle ScholarPubMed
51Pereira-Smith, OM, Smith, JR.Evidence for the recessive nature of cellular immortality. Science 1983; 221: 964–66.CrossRefGoogle ScholarPubMed
52Pereira-Smith, OM, Smith, JR.Genetic analysis of indefinite division of human cells: identification of four complementation groups. Proc NatlAcad Sci USA 1988; 85: 6042–46.CrossRefGoogle ScholarPubMed
53Olovnikov, AM.Principle of marginotomy in template synthesis of polynucleotides. Dokl Akad Nauk SSSR 1971; 201: 1496–99.Google ScholarPubMed
54Olovnikov, AM.A theory of marginotomy. J Theoret Biol 1973; 41: 181–90.CrossRefGoogle ScholarPubMed
55Medvedev, ZhA.Repetition of molecular-genetic information as a possible factor in evolutionary changes of life-span. Exp Gerontol 1972; 7: 227–34.CrossRefGoogle ScholarPubMed
56Watson, JD.Origin of concatameric T7 DNA. Nature New Biol 1972; 239: 197–201.CrossRefGoogle Scholar
57Greider, CW, Blackburn, EH.Identification of a specific telomere terminal transferase activity in Tetrahymena extracts. Cell 1985; 43: 405–13.Google Scholar
58Greider, CW, Blackburn, EH.A telomeric sequence in the RNA of Tetrahymena telomerase required for telomere repeat synthesis. Nature 1989; 337: 331–37.CrossRefGoogle ScholarPubMed
59Harley, CB, Futcher, AB, Greider, CW.Telomeres shorten during ageing of human fibroblasts. Nature 1990; 345: 458–60.Google Scholar
60Levy, MZ, Allsopp, RC, Futcher, AB, Grinder, CW, Harley, CB.Telomere end-replication problem and cell aging. JMol Biol 1992; 225: 951–60.CrossRefGoogle ScholarPubMed
61Allsopp, RC, Vaziri, H, Patterson, C. et al. Telomere length predicts replicative capacity of human fibroblasts. Proc NatlAcad Sci USA 1992, in press.Google Scholar
62Goldstein, S.Replicative senescence: the human fibroblast comes of age. Science 1990; 249: 1129–33.Google Scholar
63Lundblad, V, Szostak, JW.A mutant with a defect in telomere elongation leads to senescence in yeast. Cell 1989; 57: 633–43.CrossRefGoogle ScholarPubMed
64Moyzis, RK, Buckingham, JM, Cram, LS. et al. A highly conserved repetitive DNA sequence (TTAGGG)n present at the telomeres of human chromosomes. Proc Natl Acad Sci USA 1988; 85: 6622–26.CrossRefGoogle ScholarPubMed
65Allshire, RC, Dempster, M, Hastie, ND.Human telomeres contain at least three types of G-rich repeats distributed non-randomly. Nucleic Acid Res 1989; 17: 4611–27.CrossRefGoogle ScholarPubMed
66Meyne, J, Ratliff, RL, Moyzis, RK.Conservation of the human telomere sequence (TTAGGG)n among vertebrates. Proc Natl Acad Sci USA 1989; 86: 7049–53.Google Scholar
67Cross, SH, Allshire, RC, McKay, SJ, McGill, NI, Cooke, HJ.Cloning of human telomeres by complemenation in yeast. Nature 1989; 338: 771–74.Google Scholar
68Brown, WRA. Molecular cloning of human telomeres in yeast. Nature 1989; 338: 774–76.CrossRefGoogle ScholarPubMed
69Shippen-Lentz, D, Blackburn, EH.Functional evidence for an RNA template in telomerase. Science 1990; 247: 546.CrossRefGoogle ScholarPubMed
70Wright, WE, Shay, JW.Telomere positional effects and the regulation of cellular senescence. Trends Genet 1992; 8: 193–97.CrossRefGoogle ScholarPubMed
71Sugawara, O, Oshimura, M, Koi, M, Annab, LA, Barrett, JC. Induction of cellular senescence in immortalized cells by human chromosome 1. Science 1990; 247: 707–10.Google Scholar
72Ning, Y, Weber, JL, Killary, AM, Ledbetter, DH, Smith, JR, Pereira-Smith, OM.Genetic analysis of indefinite division in human cells: evidence for a cell senescence-related gene(s) on human chromosome 4. Proc Natl Acad Sci USA 1991; 88: 5635–39.CrossRefGoogle ScholarPubMed
73Hubbard-Smith, K, Patsalis, P, Pardinas, JR, Jha, KK, Henderson, AS, Ozer, HL.Altered chromosome 6 in immortal human fibroblasts. Mol Cell Biol 1992; 12: 2273–81.Google ScholarPubMed
74Dell'Orco, RT ed. Human diploid fibroblast-like cells as a model system for the study of senescence. Exp Gerontol 1992; 27: 359–459.CrossRefGoogle Scholar
75Murano, S, Thweatt, R, Shmookler, Reis R, Jones, RA, Moerman, EJ., Goldstein, S.Diverse gene sequences are overexpressed in Werner syndrome fibroblasts undergoing premature replicative senescence. Mol Cell Biol 1992, in press.Google Scholar
76Razin, A, Riggs, AD.DNA methylation and gene function. Science 1980; 210: 604–10.CrossRefGoogle ScholarPubMed
77Wilson, VL, Jones, PA.DNA methylation decreases in aging but not in immortal cells. Science 1983; 220: 1055–1057.CrossRefGoogle ScholarPubMed
78Fairweather, DS, Fox, M, Margison, GP.The in vitro lifespan of MRC-5 cells is shortened by 5-azacytidine-induced methylation. Exp Cell Res 1987; 168: 153–59.CrossRefGoogle Scholar
79Matsumura, T, Malik, F, Holliday, R.Levels of DNA methylation in diploid and SV40 transformed human fibroblasts. Exp Gerontol 1989; 14: 477–81.CrossRefGoogle Scholar
80Norwood, TH, Pendergrass, WR, Sprague, CA, Martin, GM.Dominance of the senescent phenotype in heterokaryons between replicative and post-replicative human fibrobLast cell. Proc Natl Acad Sci USA 1974; 71: 2231–34.CrossRefGoogle Scholar
81Stein, GH, Yanishevsky, RJ.Entry into S phase is inhibited in two immortal cell lines fused to senescent human diploid cells. Exp Cell Res 1979; 120: 155–65.Google Scholar
82Rabinovitch, PS, Norwood, TH.Comparative heterokaryon study of cellular senescence and the serum deprived state. Exp Cell Res 1980; 130: 101–109.CrossRefGoogle ScholarPubMed
83Yanishevsky, RJ, Stein, GH.Ongoing DNA synthesis continues in young human diploid cells (HDC) fused to senescent HDC, but entry into S phase is inhibited. Exp Cell Res 1980; 126: 469–72.Google Scholar
84Stein, GH, Yanishevsky, RJ, Gordon, L, Beeson, M, Carcinogen transformed human cells are inhibited from entry into S phase by fusion to senescent cells, but cells transformed by DNA tumor viruses overcome the inhibition. Proc Natl Acad Sci USA 1982; 79: 5287–91.CrossRefGoogle ScholarPubMed
85Spiering, AL, Pereira-Smith, OM, Smith, JR.Correlation between complementation group for immortality and DNA synthesis inhibitors. Exp Cell Res 1991; 195: 541–45.CrossRefGoogle Scholar
86Smith, JR.Minireview: DNA synthesis inhibitors in cellular senescence. J Gerontol Biol Sd 1990; 45: B32–35.CrossRefGoogle Scholar
87Ching, G, Wang, E.Absence of three secreted proteins and presence of a 57 kDa protein related to irreversible arrest of cell growth. Proc Natl Acad Sci USA 1988; 85: 151–55.Google Scholar
88Holliday, R.The limited proliferation of cultured diploid cells: regulation or senescence? J Gerontol Biol Sci 1990; 45: B36–41.Google Scholar
89Maier, JAM, Voulalas, P, Roeder, D, Maciag, T.Extension of the life-span of human endothelial cells by an interleukin 1 antisense oligomer. Science 1990; 249: 1570–74.Google Scholar
90Kumas, S, Millis, AJT, Baglioni, C.Expression of interleukine 1-inducible genes and production of interleukine 1 by aging human fibroblasts. Proc Natl Acad Sci USA 1992; 89: 4683–87.Google Scholar