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The role of sperm creatine kinase in the assessment of male fertility

Published online by Cambridge University Press:  02 March 2009

Gabor Huszar
Gabor Huszar*
Affiliation:
Yale University School of Medicine, Connecticut, USA
*
Gabor Huszar, The Sperm Physiology Laboratory, Department of Obstetrics and Gynecology, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06510, USA.
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Extract

The lack of reliable methods to assess sperm fertilizing potential has been a long-standing problem for infertile couples and for their physicians. The most widely used tests, the measurements of sperm concentrations, motility, velocity and morphology in the ejaculate, are of limited utility. Indeed, following intrauterine insemination, a treatment that compensates for low motile sperm concentrations, there were no significant differences found in semen parameters among those who did or did not achieve pregnancies. Other available assays probing for selected sperm functions, such as membrane integrity, acrosome enzyme activity, bovine cervical mucus penetration test, zona-free hamster oocyte penetration test and sperm binding to various carbohydrates,10–13 have all failed thus far to consistently predict male fertility. It became increasingly obvious that there was a need to identify cellular markers of sperm quality and fertilizing potential.

Type
Articles
Information
Reproductive Medicine Review , Volume 3 , Issue 3 , October 1994 , pp. 179 - 197
Copyright
Copyright © Cambridge University Press 1994

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References

1Huszar, G, DeCherney, AH. The role of intrauterine insemination in the treatment of infertile couples: the Yale experience. In: Quagliarelo, J ed. Artificial insemination, seminars in reproductive endocrinology, volume 5. New York, NY: Grune and Stratton, 1987; 5: 11–21.Google Scholar
2Nachtigall, RD, Faure, N, Glass, RH. Artificial insemination of husband's sperm. Fertil Steril 1979; 32: 141–47.Google ScholarPubMed
3Allen, NC, Herbert III, CM, Maxson, WS, Rogers, BJ, Diamond, MP, Wentz, AC. Intrauterine insemination: a critical review. Fertil Steril 1985; 44: 569–80.Google Scholar
4Jeyendran, RS, Van der Ven, HH, Perez-Pelaez, M, Crabo, BG, Zaneveld, LDJ. Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics. J Reprod Fertil 1984; 70: 219.CrossRefGoogle ScholarPubMed
5Berger, T, Marrs, RP, Moyer, DL. Comparison of techniques for selection of motile spermatozoa. Fertil Steril 1985; 43: 268–72.CrossRefGoogle ScholarPubMed
6Katz, DF, David, RO, Delandmeter, BA, Overstreet, JW. Real-time analysis of sperm motion using automatic video image digitization. Comput Math Prog Biomed 1985; 21: 173–82.CrossRefGoogle ScholarPubMed
7Overstreet, JW. Evaluation of sperm-cervical mucus interaction. Fertil Steril 1986; 45: 324–26.CrossRefGoogle ScholarPubMed
8Kruger, TF, Acosta, AA, Simmons, KF et al. New methods of evaluating sperm morphology with predictive value for human. in vitro fertilization. J Urology 1987; 30: 248–51.CrossRefGoogle ScholarPubMed
9Kennedy, WP, Kaminski, JM, Van der Ven, HH et al. A simple, clinical assay to evaluate the acrosin activity of human spermatozoa. J Androl 1989; 10: 221.CrossRefGoogle ScholarPubMed
10Rogers, BJ. The sperm penetration assay: its usefulness re-evaluated. Fertil Steril 1985; 43: 821–40.CrossRefGoogle Scholar
11Talbert, LM, Hammond, MG, Halme, J, O'Rand, M, Fryer, JG, Ekstrom, RD. Semen parameters and fertilization of human oocytes in vitro: a multivariable analysis. Fertil Steril 1987; 48: 270–77.CrossRefGoogle ScholarPubMed
12Mao, C, Grimes, AD. The sperm penetration assay: Can it discriminate between fertile and infertile men? Am J Obstet Gynecol 1988; 159: 279–86.CrossRefGoogle ScholarPubMed
13Benoff, S, Cooper, GW, Hurley, I et al. Human sperm fertilizing potentia. in vitro is correlated with differential expression of a head-specific mannose-ligand receptor. Fertil Steril 1993; 59: 854–62.CrossRefGoogle Scholar
14Aitken, RJ. Assessment of sperm function for IVF. Hum Reprod 1988; 3: 89–95.Google Scholar
15Gwatkin, RBL, Collins, JA, Jarrel, JA, Kohut, J, Milner, RA. The value of semen analysis and sperm function assays in predicting pregnancy among infertile couples. Fertil Steril 1990; 53: 693–99.CrossRefGoogle ScholarPubMed
16Tombes, RM, Shapiro, BM. Metabolite channeling: a phosphorylcreatine shuttle to mediate high energy phosphate transport between sperm mitochondrion and tail. Cell 1985; 41: 325–34.CrossRefGoogle ScholarPubMed
17Walliman, T, Moser, H, Zurbriggen, B, Wegman, G, Eppenberger, HM. Creatine kinase isoenzymes in spermatozoa. J Muscle Res and Cell Motil 1986; 7: 25–34.CrossRefGoogle Scholar
18Walliman, T, Eppenberger, HM. The subcellular compartmentation of creatine kinase isozymes as a precondition for a proposed phosphoryl-creatine circuit. Progress Clin Biol Res 1990; 344: 877–89.Google Scholar
19Huszar, G, Vigue, L, Wallimann, T. Creatine kinase in sperm: the presence of mitochondrial and brain-type creatine kinase isozymes [Abstract]. American Fertility Society Annual Meeting, Chicago, Illinois, 1985.Google Scholar
20Huszar, G, Corrales, M, Vigue, L. Correlation between sperm creatine phosphokinase activity and sperm concentrations in normospermic and oligospermic men. Gamete Res 1988; 19: 67–75.CrossRefGoogle ScholarPubMed
21Vigue, C, Vigue, L, Huszar, G. Adenosine triphosphate (ATP) concentrations and ATP/adenosine diphosphate ratios in human sperm of normospermic, oligospermic, and asthenospermic specimens and in their swim-up fractions: lack of correlation between ATP parameters and sperm creatine kinase concentrations. J Androl 1992; 13: 305–11.CrossRefGoogle ScholarPubMed
22Asseo, PP, Panidis, DK, Papadimas, JS, Ikkos, DG. Creatine kinase in seminal plasma of infertile men: activity and isoenzymes. Int J Androl 1981, 4: 431–39.CrossRefGoogle ScholarPubMed
23Kavanagh, JP, Darby, C. Creatine kinase and ATPase in human seminal fluid and prostatic fluid. J Reprod Fertil 1983; 68: 51–56.Google Scholar
24Huszar, G, Vigue, L, Corrales, M. Sperm creatine phosphokinase activity as a measure of sperm quality in normospermic, variablespermic and oligospermic men. Biol Reprod 1988; 38: 1061–66.CrossRefGoogle ScholarPubMed
25Huszar, G, Vigue, L, Corrales, M. Sperm creatine kinase activity in fertile and infertile oligospermic men. J Androl 1990; 11: 40–46.CrossRefGoogle ScholarPubMed
26DrCox, . The analysis of binary data. London: Methuen Publishing Co., 1983.Google Scholar
27Gasano, R, Orlando, C, Serio, M, Forti, G. LDH and LDH-X activity in sperm from normospermic and oligozoospermic men. Int J Androl 1991; 14(4): 257–63.Google Scholar
28Orlando, C, Krausz, C, Forti, G, Casano, R. Simultaneous measurement of sperm LDH, LDH-X, CPK activity and ATP content in normospermic and oligozoospermic men. Int J Androl 1994 (in press).CrossRefGoogle Scholar
29Chan, SYW, Wang, C. Correlation between semen adenosine triphosphate and sperm fertilizing capacity. Fertil Steril 1987; 47: 717–10.CrossRefGoogle ScholarPubMed
30Irvine, DS, Aitken, RJ. The value of adenosine triphosphate (ATP) measurements in assessing the fertilizing ability of human spermatozoa. Fertil Steril 1985; 44: 806–13.CrossRefGoogle ScholarPubMed
31Comhaire, FH, Vermeulen, L, Schoonjans, F. Reassessment of the accuracy of traditional sperm characteristics and adenosine triphosphate (ATP) in estimating the fertilizing potential of human semen in vivo. Int J Androl 1987; 10: 653–62.CrossRefGoogle ScholarPubMed
32Orlando, C, Caldini, AL, Fiorelli, G, Cuomo, S, Serio, M. ATP and ADP content of human ejaculated spermatozoa: I. relationship with semen physical parameters in normal donors and oligozoospermic patients. Int J Androl 1982; 5: 497–502.CrossRefGoogle ScholarPubMed
33Huszar, G, Vigue, L. Sperm creatine content in normospermic and oligospermic specimens. Abstract, American Society of Andrology Annual Meeting, Houston, Texas, 1989.Google Scholar
34Huszar, G, Vigue, L. Activities of sperm creatine kinase and dynein ATPase in oligospermic men. In: Orgebin-Crist, M-C, Danzo, BJ eds. Cell biology of the testis and epididymis, Volume 513. New York, NY: Annals of the New York Academy of Sciences, 1987: 602–606.Google Scholar
35Fakih, H, MacLusky, N, DeCherney, AH, Wallimann, T, Huszar, G. Enhancement of human sperm motility and velocity in vitro. Effects of calcium and creatine phosphate. Fertil Steril 1986; 46: 938–44.Google Scholar
36Huszar, G, Vigue, L. Incomplete development of human spermatozoa is associated with increased creatine phosphokinase concentrations and abnormal head morphology. Mol Reprod Dev 1993; 34: 292–98.Google Scholar
37Kruger, TF, Acosta, A, Simmons, KF, Swanson, RJ, Matta, JF, Oehninger, S. Predictive value of abnormal sperm morphology in in vitro fertilization. Fertil Steril 1988; 49: 112–17.CrossRefGoogle ScholarPubMed
38Clermont, Y. The cycle of the seminiferous epithelium in man. Am J Anat 1963; 112: 35–51.CrossRefGoogle ScholarPubMed
39Menkveld, R, Stander, FSH, Kotze, TJW, Kruger, TF, Van Zyl, JA. The evaluation of morphological characteristics of human spermatozoa according to stricter criteria. Hum Reprod 1990; 5: 586–92.Google Scholar
40Turner, DC, Maier, V, Eppenberger, HM. CK and aldolase isoenzyme transitions in cultures of chick skeletal muscle cells. Dev Biol 1974; 37: 63–89.CrossRefGoogle Scholar
41Cohen, A, Buckingham, M, Gros, F. A modified assay procedure for revealing the M-form of creatine kinase in cultured muscle cells. Exp Cell Res 1978; 115: 201–206.CrossRefGoogle ScholarPubMed
42Perriard, J-C, Caravatti, M, Perriard, E, Eppenberger, HM. Quantitation of CK isoenzyme transition in differentiating chicken embryonic breast muscle and myogenic cell cultures by immunoadsorption. Arch Biochem Biophys 1978; 191: 90–100.CrossRefGoogle Scholar
43Huszar, G, Vigue, L. Spermatogenesis-related change in the synthesis of the creatine kinase B-type and M-type isoforms in human spermatozoa. Mol Reprod Dev 1990; 25: 258–62.Google Scholar
44Huszar, G, Vigue, L, Morshedi, M. Sperm creatine phosphokinase M-isoform ratios and fertilizing potential of men: a blinded study of 84 couples treated with in vitro fertilization. Fertil Steril 1992; 57: 882–88.Google Scholar
45Huszar, G, Vigue, L, Oehninger, S. Creatine kinase immunocytochemistry of human sperm-hemizona complexes: selective binding of sperm with mature creatine kinase-staining pattern. Fertil Steril 1994; 61: 136–42.CrossRefGoogle ScholarPubMed
46Jones, R, Mann, T, Sherins, R. Peroxidative breakdown of phospholipids in human spermatozoa, spermicidal properties of fatty acid peroxides, and protective action of seminal plasma. Fertil Steril 1979; 31: 531–37.Google Scholar
47Alvarez, JG, Touchstone, JC, Blasco, L, Storey, B. Spontaneous lipid peroxidation and production of hydrogen peroxide and Superoxide in human spermatozoa. Superoxide dismutase as major enzyme protectant against oxygen toxicity. J Androl 1987; 8: 338–48.Google Scholar
48Aitken, RJ, Clarkson, JS, Hargreave, TB, Irvine, DS, Wu, CF. Analysis of the relationship between defective sperm function and the generation of reactive oxygen species in cases of oligozoospermia. J Androl 1989; 10: 214–20.Google Scholar
49Rao, B, Soufir, JC, Martin, M, David, G. Lipid peroxidation in human spermatozoa as related to midpiece abnormalities and motility. Gamete Res 1989; 24: 127–34.CrossRefGoogle ScholarPubMed
50Kessopoulou, E, Tomlinson, MJ, Barrait, CL, Bolton, AE, Cooke, IP. Origin if reactive oxygen species in human semen: spermatozoa and leukocytes. J Reprod Fertil 1992; 94: 463–70.CrossRefGoogle Scholar
51Huszar, G, Vigue, L. Correlation between the rate of lipid peroxidation and cellular maturity as measured by creatine kinase activity in human spermatozoa. J Androl 1994; 15: 71–77.CrossRefGoogle ScholarPubMed
52Honig, SC, Lipshultz, LI, Jarow, J. Significant medical pathology uncovered by a comprehensive male infertility evaluation. Fertil Steril 1994 (in press).CrossRefGoogle Scholar