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Intracytoplasmic sperm injection — ICSI

Published online by Cambridge University Press:  02 March 2009

AC Van Steirteghem ,
P Nagy ,
J Liu ,
H Joris ,
J Smitz ,
M Camus ,
P Devroey  and
M Bonduelle
AC Van Steirteghem*
Affiliation:
Centre for Reproductive Medicine, University Hospital and Medical School, Dutch-speaking Brussels Free University (Vrije Universiteit Brussel), Brussels, Belgium
P Nagy
Affiliation:
Centre for Reproductive Medicine, University Hospital and Medical School, Dutch-speaking Brussels Free University (Vrije Universiteit Brussel), Brussels, Belgium
J Liu
Affiliation:
Centre for Reproductive Medicine, University Hospital and Medical School, Dutch-speaking Brussels Free University (Vrije Universiteit Brussel), Brussels, Belgium
H Joris
Affiliation:
Centre for Reproductive Medicine, University Hospital and Medical School, Dutch-speaking Brussels Free University (Vrije Universiteit Brussel), Brussels, Belgium
J Smitz
Affiliation:
Centre for Reproductive Medicine, University Hospital and Medical School, Dutch-speaking Brussels Free University (Vrije Universiteit Brussel), Brussels, Belgium
M Camus
Affiliation:
Centre for Reproductive Medicine, University Hospital and Medical School, Dutch-speaking Brussels Free University (Vrije Universiteit Brussel), Brussels, Belgium
P Devroey
Affiliation:
Centre for Reproductive Medicine, University Hospital and Medical School, Dutch-speaking Brussels Free University (Vrije Universiteit Brussel), Brussels, Belgium
M Bonduelle
Affiliation:
Centre for Medical Genetics, University Hospital and Medical School, Dutch-speaking Brussels Free University (Vrije Universiteit Brussel), Brussels, Belgium
*
André Van Steirteghem, Centre for Reproductive Medicine, Academisch Ziekenhuis Vrije Universiteit Brussel, Laarbeeklaan 101, 1090 Brussels, Belgium.
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Extract

For more than a decade in vitro fertilization (IVF) has been successful in the treatment of couples with long-standing infertility due to various aetiologies such as tubal disease, male-factor infertility, unexplained infertility and endometriosis. The usual fertilization rate in IVF for nonmale infertility cases is 60–70% of the inseminated cumulus-oocyte complexes and in andrological infertility it is only 20–30%. The lower the number of normally fertilized oocytes, the less chance there is of available embryos, so that patients may have no embryos to transfer. It has been the experience of all centres for reproductive medicine, including our own, that a certain number of couples with male-factor infertility cannot be helped by standard IVF treatment. After insemination with progressively motile spermatozoa the number of two-pronuclear oocytes was either zero or less than 5%. Furthermore, a sizeable number of couples cannot be accepted for IVF if the number of progressively motile spermatozoa in the ejaculate is below a certain threshold number such as 500 000. In the past five years, assisted fertilization procedures have been developed to circumvent the barriers that prevent sperm access to the ooplasma, namely the zona pellucida and the ooplasmic membrane. Pregnancies and births have been reported after partial zona dissection (PZD) and subzonal insemination (SUZI). The success rate of PZD and SUZI has remained moderate: the normal fertilization rate (two-pronuclear oocytes) has never exceeded 20–25% of the micromanipulated oocytes; only two-thirds of the patients have had embryo transfers of, usually, a low number of embryos, resulting in a reduced pregnancy and take-home baby rate.

Type
Articles
Information
Reproductive Medicine Review , Volume 3 , Issue 3 , October 1994 , pp. 199 - 207
Copyright
Copyright © Cambridge University Press 1994

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References

1Tournaye, H, Devroey, P, Camus, M et al. Comparison o. in-vitro fertilization in male and tubal infertility: a 3 year survey. Hum Reprod 1992; 7: 218–22.CrossRefGoogle Scholar
2Cohen, J, Malter, H, Wright, H, Kort, H, Massey, J, Mitchell, D. Partial zona dissection of human oocytes when failure of zona pellucida penetration is anticipated. Hum Reprod 1989; 4: 435–42.CrossRefGoogle ScholarPubMed
3Malter, HE, Cohen, J. Partial zona dissection of the human oocyte: a nontraumatic method using micromanipulation to assist zona pellucida penetration. Fertil Steril 1989; 51: 139–48.CrossRefGoogle ScholarPubMed
4Cohen, J, Talansky, BE, Malter, H et al. Microsurgical fertilization and teratozoospermia. Hum Reprod 1991; 6: 118–23.CrossRefGoogle ScholarPubMed
5Cohen, J, Alikani, M, Malter, HE, Adler, A, Talansky, BE, Rosenwaks, Z. Partial zona dissection or subzonal sperm insertion: microsurgical fertilization alternatives based on evaluation of sperm and embryo morphology. Fertil Steril 1991; 56: 696–706.CrossRefGoogle ScholarPubMed
6Cohen, J, Alikani, M, Adler, A et al. Microsurgical fertilization procedures: the absence of stringent criteria for patient selection. J Assist Reprod Genet 1992; 9: 197–206.CrossRefGoogle ScholarPubMed
7Fishel, S, Jackson, P, Antinori, S, Johnson, J, Grossi, S, Versaci, C. Subzonal insemination for the alleviation of infertility. Fertil Steril 1990; 54: 828–35.CrossRefGoogle ScholarPubMed
8Fishel, S, Antinori, S, Jackson, P, Johnson, J, Rinaldi, L. Presentation of six pregnancies established by sub-zonal insemination (SUZI). Hum Reprod 1991; 6: 124–30.CrossRefGoogle ScholarPubMed
9Fishel, S, Timson, J, Lisi, F, Rinaldi, L. Evaluation of 225 patients undergoing subzonal insemination for the procurement of fertilization in vitro. Fertil Steril 1992; 57: 840–49.CrossRefGoogle ScholarPubMed
10Laws-King, A, Trounson, A, Sathananthan, H, Kola, I. Fertilization of human oocytes by microinjection of a single spermatozoon under the zona pellucida. Fertil Steril 1987; 48: 637–42.CrossRefGoogle ScholarPubMed
11Ng, SC, Bongso, A, Ratnam, S et al. Pregnancy after transfer of sperm under zona. Lancet 1988; 332: 790.CrossRefGoogle Scholar
12Ng, SC, Bongso, A, Chang, SI, Sathananthan, H, Ratnam, S. Transfer of human sperm into the perivitelline space of human oocytes after zona-drilling or zona-puncture. Fertil Steril 1989; 52: 73–78.CrossRefGoogle ScholarPubMed
13Ng, SC, Bongso, A, Ratnam, SS. Microinjection of human oocytes: a technique for severe oligoasthenoteratozoospermia. Fertil Steril 1991; 56: 1117–23.CrossRefGoogle ScholarPubMed
14Palermo, G, Joris, H, Devroey, P, Van Steirteghem, AC. Induction of acrosome reaction in human spermatozoa used for subzonal insemination. Hum Reprod 1992; 7: 248–54.CrossRefGoogle ScholarPubMed
15Palermo, G, Joris, H, Devroey, P, Van Steirteghem, AC. Pregnancies after intracytoplasmic injection of single spermatozoon into an oocyte. Lancet 1992; 340: 17–18.CrossRefGoogle ScholarPubMed
16Palermo, G, Joris, H, Derde, M-P, Camus, M, Devroey, P, Van Steirteghem, AC. Sperm characteristics and outcome of human assisted fertilization by subzonal insemination and intracytoplasmic sperm injection. Fertil Steril 1993; 59: 826–35.CrossRefGoogle ScholarPubMed
17Van Steirteghem, AC, Liu, J, Joris, H et al. Higher success rate by intracytoplasmic sperm injection than by subzonal insemination. Report of a second series of 300 consecutive treatment cycles. Hum Reprod 1993; 8: 1055–60.CrossRefGoogle ScholarPubMed
18Van Steirteghem, AC, Nagy, Z, Joris, H et al. High fertilization and implantation rates after intracytoplasmic sperm injection. Hum Reprod 1993; 8: 1061–1066.CrossRefGoogle ScholarPubMed
19Van Steirteghem, A, Liu, J, Nagy, Z et al. Use of assisted fertilization. Hum Reprod 1993; 8: 1784–85.CrossRefGoogle ScholarPubMed
20Iritani, A. Micromanipulation of gametes for in vitro assisted fertilization. Mol Reprod Dev 1991; 28: 199–207.CrossRefGoogle ScholarPubMed
21Lanzendorf, SE, Maloney, MK, Veeck, LL, Slusser, J, Hodgen, GD, Rosenwaks, Z. A preclinical evaluation of pronuclear formation by microinjection of human spermatozoa into human oocytes. Fertil Steril 1988; 49: 835–42.CrossRefGoogle ScholarPubMed
22Veeck, LL, Oehninger, S, Acosta, AA, Muasher, SJ. Sperm microinjection in a clinical in vitro fertilization program. Abstracts of the scientific papers at the forty-fifth annual meeting of the American Fertility Society, 13–16 11 1989, San Francisco, S50–51.Google Scholar
23Smitz, J, Devroey, P, Camus, M et al. The luteal phase and early pregnancy after combined GnRH-agonist/HMG treatment for superovulation in IVF or GIFT. Hum Reprod 1988; 3: 585–90.CrossRefGoogle ScholarPubMed
24Smitz, J, Devroey, P, Faguer, B, Bourgain, C, Camus, M, Van Steirteghem, AC. A prospective randomized comparison of intramuscular or intravaginal progesterone as a luteal phase and early pregnancy supplement. Hum Reprod 1992; 7: 168–75.CrossRefGoogle ScholarPubMed
25Smitz, J, Bourgain, C, Van Waesberghe, L, Camus, M, Devroey, P, Van Steirteghem, AC. A prospective randomized study on oestradiol valerate supplementation in addition to intravaginal micronized progesterone in buserelin and HMG induced superovulation. Hum Reprod 1993; 8: 40–45.CrossRefGoogle Scholar
26World Health Organization. WHO laboratory manual for the examination of human semen and sperm cervical mucus interaction, third edition. Cambridge: Cambridge University Press, 1992.Google Scholar
27Kruger, TF, Menkveld, R, Stander, FSH et al. Sperm morphologic features as a prognostic factor i. in vitro fertilization. Fertil Steril 1986; 46: 1118–23.CrossRefGoogle ScholarPubMed
28Liu, J, Nagy, Z, Joris, H, Tournaye, H, Devroey, P, Van Steirteghem, AC. Intracytoplasmic sperm injection does not require special treatment of the spermatozoa. Hum Reprod 1994; 9: 1127–30.CrossRefGoogle Scholar
29Silber, S, Nagy, ZP, Liu, J, Godoy, H, Devroey, P, Van Steirteghem, A. Conventional IVF versus ICSI (intracytoplasmic sperm injection) for patients requiring MESA (microsurgical sperm aspiration). Hum Reprod 1994; 9: (in press).CrossRefGoogle ScholarPubMed
30Verheyen, G, Pletincx, I, Van Steirteghem, AC. Effect of freezing method, thawing temperature and post-thaw dilution/washing on motility (CASA) and morphology characteristics of high-quality human sperm. Hum Reprod 1993; 8: 1678–84.CrossRefGoogle ScholarPubMed
31Staessen, C, Camus, M, Bollen, N, Devroey, P, Van Steirteghem, AC. The relationship between embryo quality and the occurrence of multiple pregnancies. Fertil Steril 1992; 57: 626–30.CrossRefGoogle ScholarPubMed
32Staessen, C, Janssenswillen, C, Van den Abbeel, E, Devroey, P, Van Steirteghem, AC. Avoidance of triplet pregnancies by elective transfer of two good quality embryoes. Hum Reprod 1993; 8: 1650–53.CrossRefGoogle Scholar
33 Van Steirteghem, AC, Van den Abbeel, E, Camus, M et al. Cryopreservation of human embryos obtained after gamete intra-Fallopian transfer and/o. in vitro fertilization. Hum Reprod 1987; 2: 593–98.CrossRefGoogle Scholar
34Camus, M, Van den Abbeel, E, Van Waesberghe, L, Wisanto, A, Devroey, P, Van Steirteghem, AC. Human embryo viability after freezing with dimethylsulfoxide as a cryoprotectant. Fertil Steril 1989; 51: 460–65.CrossRefGoogle ScholarPubMed
35Van Steirteghem, AC, Van der Elst, J, Van den Abbeel, E, Joris, H, Camus, M, Devroey, P. Cryopreservation of supernumerary multicellular human embryos obtained after intracytoplasmic sperm injection. Fertil Steril 1994; (in press).CrossRefGoogle Scholar
36Bonduelle, M, Desmyttere, S, Buysse, A et al. Prospective follow-up study of 55 children born after subzonal insemination and intracytoplasmic sperm injection. Hum Reprod 1994; 9: (in press).CrossRefGoogle ScholarPubMed