Hostname: page-component-78c5997874-g7gxr Total loading time: 0 Render date: 2024-11-09T19:55:19.117Z Has data issue: false hasContentIssue false
  • English
  • Français

Experiences with intracytoplasmic sperm injection

Published online by Cambridge University Press:  03 June 2009

Susan E Lanzendorf
Susan E Lanzendorf*
Affiliation:
The Jones Institute for Reproductive Medicine, Department of Obstetrics and Gynecology, Eastern Virginia Medical School, Norfolk, Virginia, USA
*
The Jones Institute for Reproductive Medicine, Eastern Virginia Medical School, 601 Colley Avenue, Norfolk, VA 23507, USA.
Get access Rights & Permissions [Opens in a new window]

Extract

Mammalian fertilization, whether it takes place within the female reproductive tract or within a laboratory dish, is comprised of many processes which must follow a specific sequence. The spermatozoon must bind to and pass through the zona pellucida, fuse with the oolemma and become incorporated into the cytoplasm of the oocyte. Fusion of the two gametes triggers oocyte activation, resulting in exocytosis of the cortical granules and completion of the second meiotic division of the oocyte. A block in one or more of these processes, due either to abnormalities in the spermatozoon or oocyte, may result in fertilization failure.

Type
Articles
Information
Reproductive Medicine Review , Volume 4 , Issue 2 , July 1995 , pp. 75 - 86
Copyright
Copyright © Cambridge University Press 1995

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1Bedford, MJ. Fertilization. In: Austin, CR, Short, RV eds. Reproduction in mammals. Bookl. Germ cells and fertilization. New York: Cambridge University Press, 1990:128–63.Google Scholar
2Malter, HE, Cohen, J. Partial zona dissection of the human oocytes: a non traumatic method using micromanipulation to assist zona pellucida penetration. Fertil Steril 1989; 51: 139–48.CrossRefGoogle Scholar
3Lassalle, B, Courtot, AM, Testart, J. In vitro fertilization of hamster and human oocytes by microinjection of human sperm. Gamete Res 1987; 16: 69–78.CrossRefGoogle ScholarPubMed
4Palermo, G, Joris, H, Devroey, P, Van Steirteghern, AC. Pregnancies after intracytoplasmic injection of single spermatozoon into an oocyte. Lancet 1992; 340: 17–18.CrossRefGoogle ScholarPubMed
5Lillie, FR. Studies of fertilization VI. The mechanism of fertilization in Arbacia. J Exp Zool 1914; 16: 523–90.CrossRefGoogle Scholar
6Hiramoto, Y. Microinjection of the live spermatozoa into sea urchin eggs. Exp Cell Res 1962; 27: 416–26.CrossRefGoogle ScholarPubMed
7Uehara, T, Yanagimachi, R. Microsurgical injection of spermatozoa into hamster eggs with subsequent transformation of sperm nuclei into male pronuclei. Biol Reprod 1976; 15: 467–70.CrossRefGoogle ScholarPubMed
8Uehara, T, Yanagimachi, R. Activation of hamster eggs by pricking. J Exp Zool 1977; 199: 269–74.CrossRefGoogle ScholarPubMed
9Uehara, T, Yanagimachi, R. Behavior of nuclei of testicular, caput and cauda epididymal spermatozoa injected into hamster eggs. Biol Reprod 1977; 16: 315–21.CrossRefGoogle ScholarPubMed
10Hanada, A, Chang, MC. Penetration of zona-free eggs by spermatozoa of different species. Biol Reprod 1979; 6: 300–309.CrossRefGoogle Scholar
11Yanagimachi, R, Yanagimachi, H, Rogers, BJ. The use of zona-free animal ova as a test system for the assessment of the fertilizing capacity of human spermatozoa. Biol Reprod 1976; 15: 471–76.CrossRefGoogle ScholarPubMed
12Thadani, V. Injection of sperm heads into immature rat oocytes. J Exp Zool 1979; 210: 161–68.CrossRefGoogle ScholarPubMed
13Keefer, CL, Brackett, BG, Perreault, SD. Behavior of bull sperm nuclei following microinjection into hamster oocytes. Biol Reprod 1986; 34 (suppl 1): 54.Google Scholar
14Perreault, SD, Barbee, RR, Elstein, KH, Zucker, RM, Keefer, CL. Interspecies differences in the stability of mammalian sperm nuclei assessed in vivo by sperm microinjection and in vitro by flow cytometry. Biol Reprod 1988; 39: 157–67.Google Scholar
15Perreault, SD, Zirkin, BR. Sperm nuclear decondensation in mammals: role of sperm-associated proteinase in vivo. J Exp Zool 1982; 224: 253–57.CrossRefGoogle ScholarPubMed
16Keefer, CL. Fertilization by sperm injection in the rabbit. Gamete Res 1989; 22: 59–69.CrossRefGoogle ScholarPubMed
17Perreault, SD, Wolff, RA, Zirkin, BR. The role of disulfide bond reduction during mammalian sperm nuclear decondensation in vivo. Dev Biol 1984; 101: 160–67.CrossRefGoogle ScholarPubMed
18Perreault, SD, Naish, SJ, Zirkin, BR. The timing of hamster sperm nuclear decondensation and male pronucleus formation is related to sperm nuclear disulfide bond content. Biol Reprod 1987; 36: 239–44.Google Scholar
19Naish, SJ, Perreault, SD, Foehner, AL, Zirkin, BR. DNA synthesis in the fertilizing hamster sperm nucleus: sperm template availability and egg cytoplasmiccontrol. Biol Reprod 1987; 36: 245–53.Google Scholar
20Ogura, A, Yanagimachi, R. Round spermatid nuclei injected into hamster oocytes form pronuclei and participate in syngamy. Biol Reprod 1993; 48: 219–25.CrossRefGoogle Scholar
21Tesarik, J, Sousa, M, Testart, J. Human oocyte activation after intracytoplasmic sperm injection. Human Reprod 1994; 9: 511–18.CrossRefGoogle ScholarPubMed
22Stice, SL, Robl, JM. A rabbit soluble sperm factor microinjected into mammalian oocytes causes oocyte activation. Programme for The 22nd Annual Meeting of the Society of Reproduction, Columbia, MO. Madison, WI: Society for the Study of Reproduction, 1989, p. 54.Google Scholar
23Markert, C. Fertilization of mammalian eggs by sperm injection. J Exp Zool 1983; 228: 195–201.CrossRefGoogle ScholarPubMed
24Iritani, A, Utsumi, K, Miyake, M, Hosoi, Y, Saeki, K. In vitro fertilization by a routine method and by micromanipulation. Ann NY Acad Sci 1988; 541: 583–90.CrossRefGoogle ScholarPubMed
25Lanzendorf, SE, Wolf, DP. Unpublished data, 1989.Google Scholar
26Hosoi, Y, Miyake, M, Utsumi, K, Iritani, A. Development of rabbit oocytes after microinjection of spermatozoon. Proceedings of the 11th International Congress on Animal Reproduction and Artificial Insemination, 1988, Abstract 331.Google Scholar
27Yanagida, K, Bedford, JM, Yanagimachi, R. Cleavage of rabbit eggs after microsurgical injection of testicular spermatozoa. Hum Reprod 1991; 6: 277–79.Google Scholar
28Lanzendorf, SE, Wolf, DP. Microinjection of rhesus monkey oocytes. Presented at the 45th Annual Meeting of The American Fertility Society, San Francisco, CA. Birmingham, AL: American Fertility Society, 1989: S14.Google Scholar
29Hosoi, Y, Iritani, A. Rabbit microfertilization. Mol Reprod Dev 1993; 36: 282–85.CrossRefGoogle ScholarPubMed
30Goto, K, Kinoshita, A, Takuma, Y, Ogawa, K. Fertilization of bovine oocytes by the injection of immobilized, killed spermatozoa. Vet Rec 1990; 127: 517–20.Google ScholarPubMed
31Goto, K. Bovine microfertilization and embryo transfer. Mol Reprod Dev 1993; 36: 288–90.Google Scholar
32Martin, RH, Ko, E, Rademaker, A. Human sperm chromosome complements after microinjection of hamster eggs. J Reprod Fertil 1988; 84: 179–86.CrossRefGoogle ScholarPubMed
33Iritani, A, Hosoi, Y. Microfertilization by various methods in mammalian species. In: Yoshinaga, K, Mori, T eds. Development of preimplantation embryos and their environment. New York: Liss, 1989:145–49.Google Scholar
34Yanagida, K, Yanagimachi, R, Perreault, SD, Kleinfeld, RG. Thermostability of sperm nuclei assessed by microinjection into hamster oocytes. Biol Reprod 1991; 44: 440–47.CrossRefGoogle ScholarPubMed
35Katayose, H, Matsuda, J, Yanagimachi, R. The ability of dehydrated hamster and human sperm nuclei to develop into pronuclei. Biol Reprod 1992; 47: 277–84.CrossRefGoogle ScholarPubMed
36Lanzendorf, S, Maloney, M, Ackerman, S, Acosta, A, Hodgen, G. Fertilizing potential of acrosome-defective sperm following microsurgical injection into eggs. Gamete Res 1988; 19: 329–37.CrossRefGoogle ScholarPubMed
37Lanzendorf, SE, Maloney, MK, Veeck, LL, Slusser, J, Hodgen, GD, Rosenwaks, Z. A preclinical evaluation of pronuclear formation by microinjection of human spermatozoa into human oocytes. Fertil Steril 1988; 49: 835–42.CrossRefGoogle ScholarPubMed
38Ng, S, Bongso, A, Ratnam, SS. Microinjection of human oocytes: a technique for severe oligoasthenoteratozoospermia. Fertil Steril 1991; 56: 1117–23.CrossRefGoogle ScholarPubMed
39Van Steireghem, AC, Nagy, Z, Joris, H et al. High fertilization and implantation rates after intracytoplasmic sperm injection. Hum Reprod 1933; 8: 1061–66.CrossRefGoogle Scholar
40Van Steireghem, AC, Liu, J, Joris, H et al. Higher success rate by intracytoplasmic sperm injection than by subzonal insemination. Report of a second series of 300 consecutive treatment cycles. Hum Reprod 1993; 8: 1055–60.CrossRefGoogle Scholar
41Tucker, M, Wiker, S, Massey, J. Rational approach to assisted fertilization. Hum Reprod 1993; 8: 1778–85.CrossRefGoogle ScholarPubMed
42Tsirigotis, M, Yang, D, Redgment, CJ, Nicholson, N, Felekanos, M, Craft, IL. Assisted fertilization with intracytoplasmic sperm injection. Fertil Steril 1994; 62: 781–85.Google Scholar
43Fishel, S, Timson, J, Lisi, F, Jacobson, M, Rinaldi, L, Gobetz, L. Micro-assisted fertilization in patients who have failed subzonal insemination. Hum Reprod 1994; 9: 501–505.Google Scholar
44Nagy, ZP, Joris, H, Liu, J, Staessen, C, Devroey, P, Van Steirteghem, AC. Intracytoplasmic single sperm injection of 1-day old unfertilized human oocytes. Hum Reprod 1993; 8: 2180–84.Google Scholar
45Palermo, G, Joris, H, Derde, M, Camus, M, Devroey, P, Van Steirteghem, A. Sperm characteristics and outcome of human assisted fertilization by subzonal insemination and intracytoplasmic sperm injection. Fertil Steril 1993; 59: 826–35.CrossRefGoogle ScholarPubMed
46Liu, J, Nagy, Z, Joris, H, Tournaye, H, Devroey, P, Van Steirteghem, AC. Intracytoplasmic sperm injection does not require special treatment of the spermatozoa. Hum Reprod 1994; 9: 1127–30.CrossRefGoogle Scholar
47Lundin, K, Sjogren, A, Nilsson, L, Hamberger, L. Fertilization and pregnancy after intracytoplasmic microinjection of acrosomeless spermatozoa. Fertil Steril 1994; 62: 1266–67.CrossRefGoogle ScholarPubMed
48Kullander, S, Rausing, A. On round-headed spermatozoa. Int J Fertil 1975; 20: 33–40.Google Scholar
49Bonduelle, M, Desmyttere, S, Buysse, A et al. Prospective follow-up study of 55 children born after subzonal insemination and intracytoplasmic sperm injection. Hum Reprod 1994; 9: 1765–69.Google Scholar
50Whittingham, DG, Bavister, BD. Development of hamster eggs fertilized in vitro or in vivo. J Reprod Fertil 1974; 38: 489–92.Google Scholar
51Sato, A, Yanagimachi, R. Transplantation of preimplantation hamster embryos. J Reprod Fertil 1979; 30: 329–32.Google Scholar
52Bavister, BD, Leibfried, ML, Lieberman, G. Development of preimplantation embryos of the golden hamster in a defined culture medium. Biol Reprod 1983; 28: 235–47.CrossRefGoogle Scholar
53Schini, SA, Bavister, BD. Two-cell block to development of cultured hamster embryos is caused by phosphate and glucose. Biol Reprod 1988; 39: 1183–92.Google Scholar
54MacKiernan, SH, Bavister, BD. Environmental variables influencing in vitro development of hamster 2-cell embryos to the blastocyst stage. Biol Reprod 1990; 43: 404–13.Google Scholar