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Rapid Production of Graphite Without Contamination for Biomedical AMS

Published online by Cambridge University Press:  18 July 2016

John S. Vogel
John S. Vogel*
Affiliation:
Center for Accelerator Mass Spectrometry, University of California, Lawrence Livermore National Laboratory, Box 808 L-397, Livermore, California 94551 USA
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Abstract

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The application of AMS to the detection of 14C makes possible a new class of sensitive experiments in molecular biology. Such experiments inherently produce large numbers of samples for the determination of biological variability in molecular interactions. The samples vary in 14C concentration over many orders of magnitude. We added TiH2 to aid the reduction of CO2 by zinc in a sealed tube to reproducibly make graphite without sample cross-contamination. The CO2 is transferred from a combustion tube to the reaction tube through a disposable plastic manifold. The sealed tubes are heated to a single-reaction temperature in a muffle furnace. The process is complete within 5 h. Bulk isotopic fractionation in the finished graphite is less than 0.5%.

Type
I. Sample Preparation and Measurement Techniques
Information
Radiocarbon , Volume 34 , Issue 3: Proceedings of the 14th International Radiocarbon Conference , 1992 , pp. 344 - 350
Copyright
Copyright © The American Journal of Science 

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