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How to Convert Biological Carbon Into Graphite for AMS

Published online by Cambridge University Press:  18 July 2016

Girma Getachew ,
Seung-Hyun Kim ,
Betty J Burri ,
Peter B Kelly ,
Kurt W Haack ,
Ted J Ognibene ,
Bruce A Buchholz ,
John S Vogel ,
Jonathan Modrow  and
Andrew J Clifford
Girma Getachew
Affiliation:
Department of Nutrition, University of California, Davis, California 95616, USA.
Seung-Hyun Kim
Affiliation:
Department of Nutrition, University of California, Davis, California 95616, USA.
Betty J Burri
Affiliation:
USDA, Western Human Nutrition Research Center, Davis, California 95616, USA.
Peter B Kelly
Affiliation:
Department of Chemistry, University of California, Davis, California 95616, USA.
Kurt W Haack
Affiliation:
Lawrence Livermore National Laboratory, Livermore, California 94550, USA.
Ted J Ognibene
Affiliation:
Lawrence Livermore National Laboratory, Livermore, California 94550, USA.
Bruce A Buchholz
Affiliation:
Lawrence Livermore National Laboratory, Livermore, California 94550, USA.
John S Vogel
Affiliation:
Lawrence Livermore National Laboratory, Livermore, California 94550, USA.
Jonathan Modrow
Affiliation:
Department of Nutrition, University of California, Davis, California 95616, USA.
Andrew J Clifford*
Affiliation:
Department of Nutrition, University of California, Davis, California 95616, USA.
*
Corresponding author. Email: [email protected].
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Abstract

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Isotope tracer studies, particularly radiocarbon measurements, play a key role in biological, nutritional, and environmental research. Accelerator mass spectrometry (AMS) is now the most sensitive detection method for 14C, but AMS is not widely used in kinetic studies of humans. Part of the reason is the expense, but costs would decrease if AMS were used more widely. One component in the cost is sample preparation for AMS. Biological and environmental samples are commonly reduced to graphite before they are analyzed by AMS. Improvements and mechanization of this multistep procedure is slowed by a lack of organized educational materials for AMS sample preparation that would allow new investigators to work with the technique without a substantial outlay of time and effort. We present a detailed sample preparation protocol for graphitizing biological samples for AMS and include examples of nutrition studies that have used this procedure.

Type
Articles
Information
Radiocarbon , Volume 48 , Issue 3 , 2006 , pp. 325 - 336
Copyright
Copyright © The Arizona Board of Regents on behalf of the University of Arizona 

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