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Histone Adduction with Nicotine: A Bio-Ams Study

Published online by Cambridge University Press:  18 July 2016

X. H. Wu ,
H. F. Wang ,
Y. F. Liu ,
X. Y. Lu ,
J. J. Wang  and
K. Li
X. H. Wu
Affiliation:
Department of Technical Physics, Peking University, Beijing 100871, China
H. F. Wang
Affiliation:
Department of Technical Physics, Peking University, Beijing 100871, China
Y. F. Liu
Affiliation:
Department of Technical Physics, Peking University, Beijing 100871, China
X. Y. Lu
Affiliation:
Institute of Heavy Ion Physics, Peking University, Beijing 100871, China
J. J. Wang
Affiliation:
Institute of Heavy Ion Physics, Peking University, Beijing 100871, China
K. Li
Affiliation:
Institute of Heavy Ion Physics, Peking University, Beijing 100871, China
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Abstract

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Based on the study of DNA adduction with nicotine, we have measured the mouse hepatic histone adduction with 14C-labeled nicotine in vivo by bio-accelerator mass spectrometry (bio-AMS). In the exposure of mice to nicotine, the dose range administered was from 0.2 μg to 6.0 μg kg b.w.-1, which was equivalent to a very low level of human exposure to cigarette smoke. The adducts of either histone 1 (H1) or histone 3 (H3) with nicotine in mouse liver increased markedly with increasing nicotine dose. Our results have demonstrated that in the study of protein adduction with toxic xenobiotics as a biomarker, the AMS method achieves the highest sensitivity, 4.6 × 10-17 mol (46 amol) adducts per mg H1 protein, compared to all the other methods used previously.

Type
Research Article
Information
Radiocarbon , Volume 39 , Issue 3 , 1997 , pp. 293 - 297
Copyright
Copyright © The American Journal of Science 

References

Adams, J. D., O'Mara-Adams, K. J. and Hoffmann, D. 1987 Toxic and carcinogenic agents in undiluted mainstream smoke and sidestream smoke of different types of cigarettes. Carcinogenesis 8: 729–731.Google Scholar
Arents, G. and Moudrianakis, E. N. 1993 Topography of the histone octomer surface: Repeating structural motifs utilized in the docking of nucleosomal DNA. Proceedings of the National Academy of Sciences 90: 10489–10493.CrossRefGoogle Scholar
Beland, F. A. and Poirier, M. C. 1993 Significance of DNA adduct studies in animal models for cancer molecular dosimetry and risk assessment. Environmental Health Perspectives 99: 5–10.Google Scholar
Chen, C. E., Guo, Z. Y., Yan, S. Q., Zhang, Z. F., Gong, L. H., Li, R. X. and Yu, J. K. 1990 Status of the tandem accelerator mass spectrometry facility at Peking University. Nuclear Instruments and Methods in Physics Research B52: 306–309.Google Scholar
Commerford, S. L., Carsten, A. L. and Croakite, E. P. 1982 Histone turnover within nonproliferating cells. Proceedings of the National Academy of Sciences 79: 1163–1165.Google Scholar
Ehrenberg, L. and Toernqvist, M. 1992 Use of biomarkers in epidemicology: Quantitative aspects. Toxicology Letter 64–65: 485–492.Google Scholar
Farmer, P. B. 1994 Carcinogen adducts: Use in diagnosis and risk assessment. Clinical Chemistry 40(7): 1438–1443.Google Scholar
Felton, J. S., Turteltaub, K. W., Vogel, J. S., Balhorn, R., Gledhill, B. L., Southon, J. R., Caffee, M. W., Finkel, R. C., Nelson, D. E., Proctor, I. D. and Davis, J. C. 1990 Accelerator mass spectrometry in the biomedical and environmental dosimetry. Nuclear Instruments and Methods in Physics Research B52: 517–523.Google Scholar
Grunstein, M. 1992 Histones as regulators of genes. Scientific American 267(4): 40–47.CrossRefGoogle ScholarPubMed
Gupta, R. C. 1984 Nonrandom binding of the carcinogen N-hydroxy-2-acetylaminofluorene to repetitive sequences of rat liver DNA in vivo. Proceedings of the National Academy of Sciences 81: 6943–6947.Google Scholar
Hoffmann, D., Brunnemann, K. D., Prokopczyk, B. and Djordjevic, M. D. 1994 Tobacco-specific N-nitro-samines: Chemistry, carcinogenicity, and relevance to humans. Journal of Toxicology & Environmental Health 41: 1–52.Google Scholar
Johns, E. W. 1971 The preparation and characterization of histones. In Phillips, D. M. P., ed., Histones and Nucleohistones . London and New York, Plenum Press: 10–15.Google Scholar
Li, X. S., Wang, H. F., Shi, J. Y., Wang, X. Y., Liu, Y. F., Li, K., Lu, X. Y., Wang, J. J., Liu, K. X. and Guo, Z. Y. 1996 Genotoxicity study on nicotine and nicotine-derived nitrosamine by accelerator mass spectrometry. Radiocarbon 38(2): 347–353.Google Scholar
Linder, H. and Helliger, W. 1990 Effects of eluent composition, ion-pair reagent and temperature on the separation of histiones by HPLC. Chromatography 30(9/10): 518–522.Google Scholar
Linder, H., Helliger, W. and Puschendorf, B. 1990 Separation of rat tissue histone H1 subtypes by reverse-phase HPLC. Biochemical Journal 269: 359–363.Google Scholar
Luger, K., Maeder, A. W., Richmond, R. K., Sargent, D. F. and Richmond, T. J. 1997 Crystal structure of the nucleosome core particle at 2.8a resolution. Nature 389: 251–160.Google Scholar
Stacks, P. C., Mazrimas, J. A., Corzett, M. and Balhorn, R. 1990 Reactivity and adduct formation of a polyaromatic hydrocarbon, 7-bromomethylbenz(a)anthracene, with chromatin histone proteins. Journal of Chromatography 528: 25–34.CrossRefGoogle ScholarPubMed
Turteltaub, K. W., Felton, J. S., Gledhill, B. L., Vogel, J. S., Southon, J. R., Caffee, M. W., Finkel, R. C., Nelson, D. E., Proctor, I. D. and Davis, J. C. 1990 Accelerator mass spectrometry in biomedical dosimetry: Relationship between low-level exposure and covalent binding of heterocyclic amine carcinogens to DNA. Proceedings of the National Academy of Sciences 87: 5288–5292.Google Scholar
Vogel, J. S. 1992 rapid production of graphite without contamination for biomedical AMS. In Proceedigs of the 14th International 14C Conference. Radiocarbon 34(3): 344–350.Google Scholar
Weisman, J. 1996 AMS adds realism to chemical risk assessment. Science 271: 286–287.CrossRefGoogle ScholarPubMed
Wolffe, A. P. 1992 New insights into chromatin function in transcriptional control. The Federation of American Society for Experimental Biology Journal 6: 3354–3361.CrossRefGoogle ScholarPubMed