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Experiments on 14C Dating of Contaminated Bone Using Peptides Resulting from Enzymatic Cleavage of Collagen

Published online by Cambridge University Press:  18 July 2016

Gert Jaap Van Klinken  and
Robert E. M. Hedges
Gert Jaap Van Klinken
Affiliation:
Radiocarbon Accelerator Unit, Research Laboratory for Archaeology and the History of Art Oxford University, 6 Keble Road, Oxford OX1 3QJ, UK
Robert E. M. Hedges
Affiliation:
Radiocarbon Accelerator Unit, Research Laboratory for Archaeology and the History of Art Oxford University, 6 Keble Road, Oxford OX1 3QJ, UK
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Abstract

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We describe the use of collagenase to digest ancient collagen samples to obtain a peptide mixture, and the use of reversed-phase chromatography to purify these peptides. The isolation of the tripeptides glycine-proline-hydroxyproline and glycine-proline-alanine is especially encouraging.

Type
I. Sample Preparation and Measurement Techniques
Information
Radiocarbon , Volume 34 , Issue 3: Proceedings of the 14th International Radiocarbon Conference , 1992 , pp. 292 - 295
Copyright
Copyright © The American Journal of Science 

References

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