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Experiences of Production and Homogeneity Analysis of an AMS 14C Sucrose Standard for High-Activity Measurements

Published online by Cambridge University Press:  18 July 2016

Marie Sydoff  and
Kristina Stenström
Marie Sydoff*
Affiliation:
Medical Radiation Physics, Department of Clinical Sciences Malmö, Lund University, SE-205 02 Malmö, Sweden Lund University, Department of Physics, Division of Nuclear Physics, PO Box 118, SE-221 00 Lund, Sweden
Kristina Stenström
Affiliation:
Lund University, Department of Physics, Division of Nuclear Physics, PO Box 118, SE-221 00 Lund, Sweden
*
Corresponding author. Email: [email protected]
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Abstract

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Accurate accelerator mass spectrometry (AMS) measurements rely on standards with well-known isotopic ratios. For radiocarbon measurements, a number of standards with different properties are commercially available, of which the IAEA-C6 sucrose standard with a 14C value of 150.61 pMC is the most active. When analyzing biological samples resulting from studies using 14C-labeled substances, the activity content can be up to 100 times this value. Thus, there is a need for a standard material with higher activity content than IAEA-C6 for making accurate AMS measurements on this type of sample. This paper describes the attempts of producing a standard with an activity content of about 10 times modern carbon. The material chosen has to be chemically inert, preferably non-toxic, commercially available in 14C-labeled form, and the activity must be homogeneously distributed within the material. Two different standard materials were considered: urea and sucrose. Sucrose was chosen for the new standard, since it is non-toxic, inexpensive, and organic and on combustion, forms only carbon dioxide (CO2) and water (H2O). In this paper, we discuss our experience in the production and homogeneity analysis of this material, from the crystallization of the sucrose solution to the graphitization of the samples. When using an online combustion method and a septa-sealed vial reduction method, the AMS measurements indicated that the activity was not homogeneously distributed throughout the material. Contrary to this, measurements of the sucrose solution prior to recrystallization indicated that the activity was more homogeneously distributed before than after the recrystallization. In order to determine whether the inhomogeneity depended on the graphitization method (i.e. the combustion or the reduction method) or on the material itself, 3 different graphitization methods and 2 different methods of recrystallization were tested.

Type
Sample Preparation
Information
Radiocarbon , Volume 52 , Issue 3: 20th Int. Radiocarbon Conference Proceedings , 2010 , pp. 1351 - 1357
Copyright
Copyright © 2010 by the Arizona Board of Regents on behalf of the University of Arizona 

References

Getachew, G, Kim, S-H, Burri, BJ, Kelly, PB, Haack, KW, Ognibene, TJ, Buchholz, BA, Vogel, JS, Modrow, J, Clifford, AJ. 2006. How to convert biological carbon into graphite for AMS. Radiocarbon 48(3):325–36.CrossRefGoogle Scholar
Lappin, G, Kuhnz, W, Jochemsen, R, Kneer, J, Chaudhary, A, Oosterhuis, B, Drijfhout, WJ, Rowland, M, Garner, RC. 2006. Use of microdosing to predict pharmacokinetics at the therapeutic dose: experience with 5 drugs. Clinical Pharmacology & Therapeutics 80(3):203–15.CrossRefGoogle ScholarPubMed
Ognibene, TJ, Buchholz, BA. 2005. Proposed roundtable for AMS measurement of oxalic acid with 14C/C greater than 10 modern. Poster presentation at the 10th International Conference on Accelerator Mass Spectrometry, Berkeley, California, USA, 5–10 September 2005.Google Scholar
Skog, G. 2007. The single stage AMS machine at Lund University: status report. Nuclear Instruments and Methods in Physics Research B 259(1):1–6.CrossRefGoogle Scholar
Skog, G, Hellborg, R, Erlandsson, B. 1992. Accelerator mass spectrometry at the Lund Pelletron accelerator. Radiocarbon 34(3):468–72.CrossRefGoogle Scholar
Skog, G, Rundgren, M, Sköld, P. 2010. Status of the single stage AMS machine at Lund University after 4 years of operation. Nuclear Instruments and Methods in Physics Research B 268(7–8):895–7.CrossRefGoogle Scholar
Sydoff, M, Stenström, K. 2010. 14C sample preparation for AMS microdosing studies at Lund University using online combustion and septa-sealed vials. Nuclear Instruments and Methods in Physics Research B 268(7–8):924–6.CrossRefGoogle Scholar
Vogel, JS, Southon, JR, Nelson, DE, Brown, TA. 1984. Performance of catalytically condensed carbon for use in accelerator mass spectrometry. Nuclear Instruments and Methods in Physics Research B 5(2):289–93.CrossRefGoogle Scholar