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Evaluation of Possible Contamination Sources in the 14C Analysis of Bone Samples by FTIR Spectroscopy

Published online by Cambridge University Press:  18 July 2016

Marisa D'Elia ,
Gabriella Gianfrate ,
Gianluca Quarta ,
Livia Giotta ,
Gabriele Giancane  and
Lucio Calcagnile
Marisa D'Elia
Affiliation:
Department of Engineering of Innovation and CEDAD, University of Lecce, Lecce, Italy
Gabriella Gianfrate
Affiliation:
Department of Engineering of Innovation and CEDAD, University of Lecce, Lecce, Italy
Gianluca Quarta*
Affiliation:
Department of Engineering of Innovation and CEDAD, University of Lecce, Lecce, Italy
Livia Giotta
Affiliation:
Department of Materials Science, University of Lecce, Lecce, Italy
Gabriele Giancane
Affiliation:
Department of Engineering of Innovation and CEDAD, University of Lecce, Lecce, Italy
Lucio Calcagnile
Affiliation:
Department of Engineering of Innovation and CEDAD, University of Lecce, Lecce, Italy
*
Corresponding author. Email: [email protected]
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Abstract

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In the sample preparation laboratory of CEDAD (CEnter for DAting and Diagnostics) of the University of Lecce, a protocol for the quality control of bone samples based on infrared spectroscopy has been set up. The protocol has been recently developed as a check-in test with the aim to identify the presence of collagen in the samples, assess its preservation status, and determine whether the submitted bone samples are suitable for accelerator mass spectrometry (AMS) radiocarbon measurements or not. We discuss in this paper the use of infrared-based techniques to identify the presence of “contaminants” such as restoration and consolidation materials, humic acids, and soil carbonates, which, if not removed by the sample processing chemistry, can be sources of exogenous carbon and can thus influence the accuracy of the 14C determinations.

Bone samples recovered in well-defined and previously dated archaeological contexts were intentionally contaminated, submitted to the standard method for collagen extraction and purification, and then characterized by means of Fourier transform infrared (FTIR) spectroscopy analyses performed in attenuated total reflection (ATR) mode before being combusted, converted to graphite, and measured by AMS. The study shows that the ATR-FTIR technique is an extremely powerful method for the identification of both the collagen and its contaminants and can supply important information during the selection and processing of samples to be submitted for 14C dating.

Type
Articles
Information
Radiocarbon , Volume 49 , Issue 2 , 2007 , pp. 201 - 210
Copyright
Copyright © 2007 by the Arizona Board of Regents on behalf of the University of Arizona 

References

Berna, F, Matthews, A, Weiner, S. 2004. Solubilities of bone mineral from archaeological sites: the recrystallization window. Journal of Archaeological Science 31(7):867–82.Google Scholar
Calcagnile, L, Quarta, G, D'Elia, M. 2005. High-resolution accelerator-based mass spectrometry: precision, accuracy and background. Applied Radiation and Isotopes 62(4):623–9.Google Scholar
Collins, MJ, Nielsen-Marsh, CM, Hiller, J, Smith, CI, Roberts, JP, Prigodich, RV, Wess, TJ, Csapò, J, Millard, AR, Turner-Walker, G. 2002. The survival of organic matter in bone: a review. Archaeometry 44(3):383–94.Google Scholar
D'Elia, M, Calcagnile, L, Quarta, G, Sanapo, C, Laudisa, M, Toma, U, Rizzo, A. 2004. Sample preparation and blank values at the AMS radiocarbon facility of the University of Lecce. Nuclear Instruments and Methods in Physics Research B 223–224:278–83.Google Scholar
Derrick, MR, Stulik, DC, Landry, JM. 1999. Infrared Spectroscopy in Conservation Science. Los Angeles: The Getty Conservation Institute. 248 p.Google Scholar
Doyle, BB, Bendit, EG, Blout, ER. 1975. Infrared spectroscopy of collagen and collagen-like polypeptides. Bio-polymers 14(5):937–57.Google Scholar
Fernández-Jalvo, Y, Sánchez-Chillón, B, Andrews, P, Fernández-López, S, Alcalá Martínez, L. 2002. Morphological taphonomic transformations of fossil bones in continental environments, and repercussions on their chemical composition. Archaeometry 44(3):353–61.Google Scholar
Friess, W, Lee, G. 1996. Basic thermoanalytical studies of insoluble collagen matrices. Biomaterials 17(23):2289–94.CrossRefGoogle ScholarPubMed
Gianfrate, G, D'Elia, M, Quarta, G, Giotta, L, Valli, L, Calcagnile, L. 2007. Qualitative application based on IR spectroscopy for bone sample quality control in radiocarbon dating. Nuclear Instruments and Methods in Physics Research B 259(1):316–9.CrossRefGoogle Scholar
Hedges, REM. 2002. Bone diagenesis: an overview of processes. Archaeometry 44(3):319–28.Google Scholar
Hedges, REM, van Klinken, GJ. 1992. A review of current approaches in the pretreatment of bone for radiocarbon dating for AMS. Radiocarbon 34(3):279–91.Google Scholar
Johnson, JS. 1994. Consolidation of archaeological bone: a conservation perspective. Journal of Field Archaeology 21:221–33.Google Scholar
Longin, R. 1971. New method of collagen extraction for radiocarbon dating. Nature 230(5291):241–2.CrossRefGoogle ScholarPubMed
Lozano, LF, Peña-Rico, MA, Heredia, A, Ocotlán-Flores, J, Gómez-Cortés, A, Velazquez, R, Belío, IA, Bucio, L. 2003. Thermal analysis study of human bone. Journal of Materials Science 38(23):4777–82.CrossRefGoogle Scholar
Nielsen-Marsh, CM, Hedges, REM. 2000. Patterns of diagenesis in bone I: the effects of site environments. Journal of Archaeological Science 27(12):1139–50.Google Scholar
Quarta, G, Calcagnile, L, D'Elia, M, Rizzo, A, Ingravallo, E. 2004. AMS radiocarbon dating of “Grotta Cappuccini” in southern Italy. Nuclear Instruments and Methods in Physics Research B 223–224:705–8.Google Scholar
Quarta, G, D'Elia, M, Butalag, K, Maruccio, L, Demortier, G, Calcagnile, L. 2006. An integrated accelerator mass spectrometry radiocarbon dating and ion beam analysis approach for the study of archaeological contexts. Applied Physics A 83(4):605–9.Google Scholar
Reiche, I, Vignaud, C, Menu, M. 2002. The crystallinity of ancient bone and dentine: new insights by transmission electron microscopy. Archaeometry 44(3):447–59.Google Scholar
Roberts, SJ, Smith, CI, Millard, A, Collins, MJ. 2002. The taphonomy of cooked bone: characterizing boiling and its physico-chemical effects. Archaeometry 44(3):485–94.Google Scholar
Shahack-Gross, R, Bar-Yosef, O, Weiner, S. 1997. Black-coloured bones in Hayonim Cave, Israel: differentiating between burning and oxide staining. Journal of Archaeological Science 24(5):439–46.Google Scholar
Stiner, MC, Kuhn, SL, Weiner, S, Bar-Yosef, O. 1995. Differential burning, recrystallization, and fragmentation of archaeological bone. Journal of Archaeological Science 22(2):223–37.CrossRefGoogle Scholar
Stiner, MC, Kuhn, SL, Surovell, TA, Goldberg, P, Meignen, L, Weiner, S, Bar-Yosef, O. 2001. Bone preservation in Hayonim Cave (Israel): a macroscopic and mineralogical study. Journal of Archaeological Science 28(6):643–59.CrossRefGoogle Scholar
Susi, H, Ard, JS, Carroll, RJ. 1971. Hydration and denaturation of collagen as observed by infrared spectroscopy. Journal of American Leather Chemists Association 66(11):508–19.Google Scholar
Trueman, CNG, Behrensmeyer, AK, Tuross, N, Weiner, S. 2004. Mineralogical and compositional changes in bones exposed on soil surfaces in Amboseli National Park, Kenya: diagenetic mechanisms and the role of sediment pore fluids. Journal of Archaeological Science 31(6):721–9.CrossRefGoogle Scholar
van Klinken, GJ. 1999. Bone collagen quality indicators for palaeodietary and radiocarbon measurements. Journal of Archaeological Science 26(6):687–95.Google Scholar
van Klinken, GJ, Hedges, REM. 1995. Experiments on collagen-humic interactions: speed of humic uptake, and effects of diverse chemical treatments. Journal of Archaeological Science 22(2):263–70.Google Scholar
Warren, RJ, Smith, WE, Tillman, WJ. 1969. Internal reflectance spectroscopy and the determination of the degree of denaturation of insoluble collagen. Journal of American Leather Chemists Association 64(1):4–11.Google Scholar
Weiner, S, Bar-Yosef, O. 1990. States of preservation of bones from prehistoric sites in the Near East: a survey. Journal of Archaeological Science 17(2):187–96.Google Scholar
Wright, LE, Schwarcz, HP. 1996. Infrared and isotopic evidence for diagenesis of bone apatite at Dos Pilas, Guatemala: palaeodietary implications. Journal of Archaeological Science 23(6):933–44.Google Scholar
Yizhaq, M, Mintz, G, Cohen, I, Khalaily, H, Weiner, S, Boaretto, E. 2005. Quality controlled radiocarbon dating of bones and charcoal from the early Pre-Pottery Neolithic B (PPNB) of Motza (Israel). Radiocarbon 47(2): 193–206.Google Scholar