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Assessing the Effect of Sterilization on the Radiocarbon Signature of Freshwater Dissolved Organic Carbon

Published online by Cambridge University Press:  18 July 2016

Andy Baker ,
Pauline Gulliver ,
Phillipa Ascough ,
Jessie Roe  and
John Bridgeman
Andy Baker*
Affiliation:
Connected Waters Initiative Research Centre, University of New South Wales, 110 King St, Manly Vale 2093, Australia. Also: National Centre for Groundwater Research and Training.
Pauline Gulliver
Affiliation:
NERC Radiocarbon Facility (Environment), SUERC, Rankine Avenue, Scottish Enterprise Technology Park, East Kilbride G75 0QF, United Kingdom.
Phillipa Ascough
Affiliation:
SUERC, Rankine Avenue, Scottish Enterprise Technology Park, East Kilbride G75 0QF, United Kingdom.
Jessie Roe
Affiliation:
Civil Engineering, University of Birmingham, Edgbaston, Birmingham B15 2TT, United Kingdom. Severn Trent Water Ltd, Severn Trent Centre, PO Box 5309, Coventry CV3 9FH, United Kingdom.
John Bridgeman
Affiliation:
Civil Engineering, University of Birmingham, Edgbaston, Birmingham B15 2TT, United Kingdom.
*
Corresponding author. Email: [email protected].
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Abstract

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Radiocarbon analysis of freshwater dissolved organic carbon (DOC) involves substantial sample pretreatment, including an initial rotary evaporation stage necessary to concentrate large volumes of freshwater sample. This may lead to a health risk from the exposure to pathogens, and there is the additional concern that the warm conditions during the rotary evaporation stage may provide ideal growing conditions for some pathogens. To remove any pathogen risk in water samples, boiling or autoclaving can be undertaken. However, to date, no studies have been undertaken to investigate whether boiling will alter the 14C signature of dissolved organic carbon. Here, we analyze the effect of sterilization on 9 contrasting river water samples. Comparing filtered, filtered and boiled, and filtered and sterilized dissolved organic matter, we observe that both boiling and autoclaving increases the uncertainty associated with the 14C and 13C of DOC, that the 14C and 13C changes are not unidirectional, and that they are not related to original DOC composition. Neither sterilization method is recommended unless essential, in which instance we recommend a 3σ uncertainty on 14C and that the 13C is not considered representative of the original sample.

Type
Articles
Information
Radiocarbon , Volume 53 , Issue 4 , 2011 , pp. 659 - 667
Copyright
Copyright © The Arizona Board of Regents on behalf of the University of Arizona 

References

Aiken, GR, Hsu-Kim, H, Ryan, JN. 2011. Influence of dissolved organic matter on the environmental fate of metals, nanoparticles, and colloids. Environmental Science and Technology 45(8):3196–201.Google Scholar
Aufdenkampe, AK, Mayorga, E, Raymond, PA, Melack, JM, Doney, SC, Alin, SR, Aalto, RE, Yoo, K. 2011. Riverine coupling of biogeochemical cycles between land, oceans, and atmosphere. Frontiers in Ecology and Environment 9(1):53–60.Google Scholar
Baker, A, Tipping, E, Thacker, SA, Gondar, D. 2008. Relating dissolved organic matter fluorescence and functional properties. Chemosphere 73(11):1765–72.CrossRefGoogle ScholarPubMed
Battin, TJ, Kaplan, LA, Findlay, S, Hopkinson, CS, Marti, E, Packman, AI, Newbold, JD, Sabater, H. 2008. Biophysical controls on organic carbon fluxes in fluvial networks. Nature Geoscience 1:95–100.Google Scholar
Battin, TJ, Luyssaert, S, Kaplan, LA, Aufdenkampe, AK, Richter, A, Tranvik, LJ. 2009. The boundless carbon cycle. Nature Geoscience 2:598–600.Google Scholar
Bieroza, M, Baker, A, Bridgeman, J. 2009. Relating freshwater organic matter fluorescence to organic carbon removal efficiency in drinking water treatment. Science of the Total Environment 407(5):1765–74.CrossRefGoogle ScholarPubMed
Billett, MF, Moore, TR. 2008. Supersaturation and evasion of CO2 and CH4 in surface waters at Mer Bleue peatland, Canada. Hydrological Processes 22(12):2044–54.Google Scholar
Billett, MF, Garnett, MH, Harvey, F. 2007. UK peatland streams release old carbon dioxide to the atmosphere and young dissolved organic carbon to rivers. Geophysical Research Letters 34: L23401, doi::10.1029/2007GL031797.Google Scholar
Cole, JJ, Caraco, NF. 2001. Carbon in catchments: connecting terrestrial carbon losses with aquatic metabolism. Marine and Freshwater Research 52(1):101–10.Google Scholar
Cole, JJ, Prairie, YT, Caraco, NF, McDowell, WH, Tranvik, LJ, Striegl, RG, Duarte, CM, Kortelainen, P, Downing, JA, Middelburg, JJ, Melack, J. 2007. Plumbing the global carbon cycle: integrating inland waters into the terrestrial carbon budget. Ecosystems 10(1):171–84.Google Scholar
Cory, RM, McKnight, DM, Chin, Y-P, Miller, P, Jaros, CL. 2007. Chemical characteristics of fulvic acids from Arctic surface waters: microbial contributions and photochemical transformations. Journal of Geophysical Research 112: G045S51, doi:10.1029/2006JG000343.CrossRefGoogle Scholar
Evans, CD, Freeman, C, Cork, LG, Thomas, DN, Reynolds, B, Billett, MF, Garnett, MH, Norris, D. 2007. Evidence against recent climate-induced destabilisation of soil carbon from 14C analysis of riverine dissolved organic matter. Geophysical Research Letters 34: L07407, doi::10.1029/2007GL029431.CrossRefGoogle Scholar
Freeman, S, Bishop, P, Bryant, CL, Cook, GT, Dougans, A, Ertunç, T, Fallick, AE, Ganeshram, RS, Maden, C, Naysmith, P, Schnabel, C, Scott, EM, Summerfield, MA, Xu, S. 2007. The SUERC AMS laboratory after 3 years. Nuclear Instruments and Methods in Physics Research B 259(1):66–70.Google Scholar
Freeman, S, Cook, GT, Dougans, AB, Naysmith, P, Wilkeen, KM, Xu, S. 2010. Improved SSAMS performance. Nuclear Instruments and Methods in Physics Research B 268(7–8):715–7.Google Scholar
Gulliver, P, Waldron, S, Scott, EM, Bryant, CL. 2010. The effect of storage in the radiocarbon, stable carbon and nitrogen isotopic signatures and concentrations of riverine DOM. Radiocarbon 52(3):1113–22.Google Scholar
Hudson, NJ, Baker, A, Ward, D, Brunsdon, C, Reynolds, DM, Carliell-Marquet, C, Browning, S. 2008. Can fluorescence spectrometry be used as a surrogate for the Biochemical Oxygen Demand (BOD) test in water quality assessment? An example from South West England. Science of the Total Environment 391(1):149–58.CrossRefGoogle ScholarPubMed
Hudson, NJ, Baker, A, Reynold, DM, Carliell-Marquet, CM, Ward, D. 2009. Changes in freshwater organic matter fluorescence intensity with freezing/thawing and dehydration/rehydration. Journal of Geophysical Research Biogeosciences 114: G00F08, doi:10.1029/2008JG000915.Google Scholar
Kalbitz, K, Schwesig, D, Rethemeyer, J, Matzner, E. 2005. Stabilization of dissolved organic matter by sorption to the mineral soil. Soil Biology and Biochemistry 37(7):1319–31.Google Scholar
Pollard, PC, Ducklow, H. 2011. Ultrahigh bacterial production in a cutrophic subtropical Australian river: Does viral lysis short-circuit the microbial loop? Limnology and Oceanography 56(3):1115–29.Google Scholar
Raymond, PA, McClelland, JW, Holmes, RM, Zhulidov, AV, Mull, K, Peterson, BJ, Striegl, RG, Aiken, GR, Gurtovaya, TY. 2007. Flux and age of dissolved organic carbon exported to the Arctic Ocean: a carbon isotopic study of the five largest arctic rivers. Global Biogeochemical Cycles 21: GB4011, doi::10.1029/2007GB002934.Google Scholar
Roe, J, Baker, A, Bridgeman, J. 2009. Relating organic matter character to trihalomethane formation potential: a data mining approach. Water Science and Technology: Water Supply 8(6):717–23.Google Scholar
Schwesig, D, Göttlein, A, Haumaier, L, Blasek, R, Ilgen, G. 1999. Soil organic matter extraction using water at high temperature and elevated pressure (ASE) as compared to conventional methods. International Journal of Environmental Analytical Chemistry 73(4):253–68.Google Scholar
Spencer, RGM, Pellerin, BA, Bergamaschi, BA, Downing, BD, Kraus, TEC, Smart, DR, Dahlgren, RA, Hernes, PJ. 2007. Diurnal variability in riverine dissolved organic matter composition determined by in situ optical measurement in the San Joaquin River (California, USA). Hydrological Processes 21(23):3181–9.CrossRefGoogle Scholar
Tipping, E, Billett, MF, Bryant, CL, Buckingham, S, Thacker, SA. 2010. Sources and ages of dissolved organic matter in peatland streams: evidence from chemistry mixture modeling and radiocarbon data. Biogeochemistry 100(1–3):121–37.Google Scholar
Wilkinson, KJ, Balnois, E, Leppard, GG, Buffle, J. 1999. Characteristic features of the major components of freshwater colloidal organic matter revealed by transmission electron and atomic force microscopy. Colloids and Surfaces A: Physicochemical and Engineering Aspects 155(2–3):287–310.Google Scholar
World Health Organization [WHO]. 2004. Laboratory Biosafety Manual. 3rd edition. Geneva: WHO.Google Scholar
Worrall, F, Burt, TP. 2007. Trends in DOC concentration in Great Britain. Journal of Hydrology 346(3–4):81–91.Google Scholar