Hostname: page-component-78c5997874-xbtfd Total loading time: 0 Render date: 2024-11-19T06:48:36.218Z Has data issue: false hasContentIssue false

Stable Isotope and Radiocarbon Analysis in Animal Bones from the Prehistoric Settlement of Dispilio, Kastoria Lake, Northern Greece

Published online by Cambridge University Press:  27 November 2017

Paraskevi Chantzi*
Affiliation:
Laboratories of Stable Isotopes and Radiocarbon, Institute of Nanoscience and Nanotechnology, National Centre for Scientific Research. “Demokritos”, 15310 Agia Paraskevi, Attica, Greece
Elissavet Dotsika
Affiliation:
Laboratories of Stable Isotopes and Radiocarbon, Institute of Nanoscience and Nanotechnology, National Centre for Scientific Research. “Demokritos”, 15310 Agia Paraskevi, Attica, Greece Institute of Geosciences and Earth Resources, Via G. Moruzzi 1, 56124 Pisa, Italy
Konstantinos Albanakis
Affiliation:
Department of Physical and Environmental Geography, School of Geology, Aristotle University, Thessaloniki, Greece
Konstantinos Kostakis
Affiliation:
School of History and Archaeology, Aristotle University, Thessaloniki, Greece
*
*Corresponding author. Email: [email protected].

Abstract

Wild boar and roe deer samples from an excavation in Dispilio, Greece, were subjected to collagen extraction protocols to reconstruct the paleoecological regime. Radiocarbon (14C) analysis suggested the Middle/Late Neolithic period and the database was updated with collagen samples. The 14C model concluded to a possible local deforestation effect in the settlement subbasin confirmed by sediment δ13C and δ15N values. Carbon isotope values in collagen samples concluded in C3 plant type. Both carbon and nitrogen isotopes indicated the differences in dietary habits and/or metabolic system between the two Late Neolithic I species. Roe deer samples were classified as purely herbivorous. δ15N values of wild boar collagen samples from Dispilio reflected a diet mainly characterized by terrestrial protein. Compared to literature data, wild boar samples from the Dispilio excavation concluded that the animals might have lived close to the settlement where their diet could be supplemented by a consistent animal protein fraction. Finally, it is concluded that rainfall is an important factor that affects plant, and consequently animal, δ15N values. Therefore, the rainfall regime should always be considered in paleodietary studies.

Type
Applications
Copyright
© 2017 by the Arizona Board of Regents on behalf of the University of Arizona 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

Selected Papers from the 8th Radiocarbon & Archaeology Symposium, Edinburgh, UK, 27 June–1 July 2016

References

REFERENCES

Ambrose, SH. 1991. Effects of diet, climate and physiology on nitrogen isotope abundances in terrestrial foodwebs. J. Archaeol. Sci. 18(3):293317.CrossRefGoogle Scholar
Ammerman, A, Biagi, P, editors. 2003. The Widening Harvest. The Neolithic Transition in Europe: Looking Back, Looking Forward. Boston: Archaeological Institute of America.Google Scholar
Angerosa, F, Bréas, O, Contento, S, Guillou, C, Reniero, F, Sada, E. 1999. Application of stable isotope ratio analysis to the characterization of the geographical origin of olive oils. Journal of Agriculture and Food Chemistry 47:10131017.Google Scholar
Balasse, M, Bocherens, H, Mariotti, A, Ambrose, SH. 2001. Detection of dietary changes by intra-tooth carbon and nitrogen analysis: an experimental study of dentine collagen of cattle (bos taurus). J. Archaeol. Sci. 28:235245.Google Scholar
Balasse, M, Evin, A, Tornero, C, Radu, V, Fiorillo, D, Popovici, D, Andreescu, R, Dobney, K, Cucchi, T, Bălăşescu, A. 2016. Wild, domestic and feral? Investigating the status of suids in the Romanian Gumelniţa (5th mil. cal BC) with biogeochemistry and geometric morphometrics. Journal of Anthropological Archaeology 42:2736.CrossRefGoogle Scholar
Barker, G. 2006. The Agricultural Revolution in Prehistory. Why Did Foragers Become Farmers? Oxford: Oxford University Press.CrossRefGoogle Scholar
Bocherens, H, Drucker, DG, Billiou, D, Patou-Mathis, M, Vandermeersch, B. 2005. Isotopic evidence for diet and subsistence pattern of the Saint-Césaire I Neanderthal: review and use of a multi-source mixing model. J. Hum. Evol. 49:7187.CrossRefGoogle ScholarPubMed
Bogaard, A, Heaton, THE, Poulton, P, Merbach, I. 2007. The impact of manuring on nitrogen isotope ratios in cereals: archaeological implications for reconstruction of diet and crop management practices. J. Archaeol. Sci. 24:335343.CrossRefGoogle Scholar
Bréas, O, Guillou, C, Reniero, F, Sada, E, Angerosa, F. 1998. Oxygen-18 measurement by continuous flow pyrolysis/isotope ratio mass spectrometry of vegetable oils. Rapid Communications in Mass Spectrometry 12(4):188192.Google Scholar
Canti, M, Huisman, DJ. 2015. Scientific advances in geoarchaeology during the last twenty years. J. Archaeol. Sci. 56:96108.Google Scholar
Caut, S, Angulo, E, Courchamp, F. 2009. Variation in discrimination factors (δ15N and δ13C): the effect of diet isotopic values and applications for diet reconstruction. J Appl Ecol 46:443445.Google Scholar
Chapman, B, Trani, M. 2007. Feral pig (sus scrofa). In Trani M, Ford W, Chapman B, editors. The Land Manager’s Guide to Mammals of the South. Durham, NC: The Nature Conservancy and the US Forest Service, Southern Region. p 540544.Google Scholar
Chantzi, P, Poutouki, AE, Dotsika, E. 2016. D-O-C Stable isotopes, 14C radiocarbon and radiogenic isotope techniques applied in wine products for geographical origin and authentication. In: Morata A, Loira I, editors. Grape and Wine Biotechnology. InTech. DOI: 39510.5772/64933.Google Scholar
Chatzitoulousis, SI. 2008. Woodworking technology at the Neolithic lakeside settlement of Dispilio, Kastoria. Anaskamma 1:93123. In Greek with English abstract.Google Scholar
Chourmouziadis, G. 2002. The Dispilio excavations. In Chourmouziadis G, editor. Dispilio, 7500 Years After. Thessaloniki: University Studio Press. p 1123. In Greek.Google Scholar
Chourmouziadis, G, Sofronidou, M. 2007. Dispilio near Kastoria: the prehistoric lake settlement. In Valavanis P, editor. Great Moments in Greek Archaeology. Los Angeles: J Paul Getty Museum. p 272283.Google Scholar
Codron, J, Codron, D, Lee-Thorp, JA, Sponheimer, M, Bond, WJ, de Ruiter, D, Grant, R. 2005. Taxonomic, anatomical, and spatio-temporal variations in the stable carbon and nitrogen isotopic compositions of plants from an African savanna. J. Archaeol. Sci. 32:17571772.CrossRefGoogle Scholar
Cohen, MN. 2008. Implications of the NDT for worldwide health and mortality in prehistory. In Bocquet-Appel J-P, Bar-Yosef O, editors. The Neolithic Demographic Transition and its Consequences. Springer. New York. p 481500.Google Scholar
Cormie, AB, Schwarcz, HP. 1995. Effects of climate on deer bone δ15N and δ13C: lack of precipitation effects on δ15N. Geochimica et Cosmochimica Acta 60:41614166.Google Scholar
Darimont, CT, Reimchen, TE. 2002. Intra-hair stable isotope analysis implies seasonal shift to salmon in gray wolf diet. Can. J. Zool. 80:16381642.Google Scholar
DeNiro, MJ, Epstein, S. 1978. Influence of diet on the distribution of carbon isotopes in animals. Geochimica et Cosmochimica Acta 42:495506.Google Scholar
DeNiro, MJ, Epstein, S. 1981. Influence of diet on the distribution of nitrogen isotopes in animals. Geochimica et Cosmochimica Acta 45:341351.CrossRefGoogle Scholar
DeNiro, MJ. 1985. Postmortem preservation and alteration of in vivo bone collagen isotope ratios in relation to paleodietary reconstruction. Nature 317:806809.Google Scholar
Ecker, M, Bocherens, H, Julien, MA, Rivals, F, Raynal, JP, Moncel, MH. 2013. Middle Pleistocene ecology and Neanderthal subsistence: insights from stable isotope analyses in Payre (Ardèche, southeastern France). Journal of Human Evolution 65(4):363373.Google Scholar
Fornander, E, Eriksson, G, Lidén, K. 2008. Wild at heart: approaching Pitted Ware identity, economy and cosmology through stable isotopes in skeletal material from the Neolithic site Korsnäs in eastern central Sweden. Journal of Anthropological Archaeology 27(3):281297.Google Scholar
Fraser, RA, Bogaard, A, Heaton, T, Charles, M, Jones, G, Christensen, BT, Halstead, P, Merbach, I, Poulton, PR, Sparkes, D, Styring, AK. 2011. Manuring and stable nitrogen isotope ratios in cereals and pulses: towards a new archaeobotanical approach to the inference of land use and dietary practices. J. Archaeol. Sci. 28:27902804.Google Scholar
Goude, G, Fontugne, M. 2016. Carbon and nitrogen isotopic variability in bone collagen during the Neolithic period: Influence of environmental factors and diet. J. Archaeol. Sci. 70:117131.Google Scholar
Gröcke, DR, Bocherens, H, Mariotti, A. 1998. Annual rainfall and nitrogen-isotope correlation in macropod collagen: application as a palaeoprecipitation indicator. Earth and Planetary Science Letters 153:279286.CrossRefGoogle Scholar
Heaton, TH, Vogel, JC, von la Chevallerie, G, Collett, G. 1986. Climatic influence on the isotopic composition of bone nitrogen. Nature 322:822823.CrossRefGoogle Scholar
Heaton, THE. 1987. The 15N/14N ratios of plants in South Africa and Namibia: relationship to climate and coastal/saline environments. Oecologia 74:236246.CrossRefGoogle ScholarPubMed
Hedges, REM, Clement, JG, Thomas, CDL, O’ Connel, TC. 2007a. Collagen turnover in the adult femoral mid-shaft: modeled form anthropogenic radiocarbon tracer measurements. American Journal of Physical Anthropology 133(2):808816.Google Scholar
Hedges, REM, Reynard, LM. 2007b. Nitrogen isotopes and the trophic level of humans in archaeology. J. Archaeol. Sci. 34:12401251.CrossRefGoogle Scholar
Hershkovitz, I, Gopher, A. 2008. Demographic, biological and cultural aspects of the Neolithic revolution: a view from the Southern Levant. In: Bocquet-Appel J-P., Bar-Yosef O, editors. The Neolithic Demographic Transition and its Consequences. New York: Springer. p 441479.CrossRefGoogle Scholar
Hoefs, J. 2009. Stable Isotope Geochemistry. 6th edition. Berlin: Springer. 286 p.Google Scholar
Iacumin, P, Bocherens, H, Mariotti, A, Longinelli, A. 1996. Oxygen isotope analyses of co-existing carbonate and phosphate in biogenic apatite: a way to monitor diagenetic alteration of bone phosphate? Earth Planet. Sci. Lett. 142:16.Google Scholar
Karkanas, P. 2002. Micromorphological studies in Greek Prehistoric sites: the new insights in the interpretation of the archaeological record. Geoarchaeology 17(3):237259.Google Scholar
Keaveney, EM, Reimer, PJ. 2012. Understanding the variability in freshwater radiocarbon reservoir offsets: a cautionary tale. J. Archaeol. Sci. 39(5):13061316.Google Scholar
Keramopoulos, A. 1932. Excavations and Investigations at Upper Macedonia, Archaeologiki Ephemeris. p 48–133. In Greek.Google Scholar
Kokkinidou, D, Trantalidou, K. 1991. Neolithic and Bronze Age settlements in western Macedonia. The Annual of British School at Athens 86:93106.Google Scholar
Kouli, K. 2002. Palaeoenvironmental and palaeoecological reconstruction of the area of the Dispilio Neolithic settlement, Kastoria lake, northern Greece. National and Kapodistrian University of Athens, Edition of the Department of Geology and Geoenvironment. Gaia 17:149 Athens. In Greek with English summary.Google Scholar
Leránoz, I. 1983. Sobre la relación del jabalí (Sus scrofa L.) con la agricultura en Navarra septentrional. In: Proceedings of XV Congress International de. Fauna Cinegética y Silvestre, Trujillo, Cáceres, Spain. p 639-45.Google Scholar
Lidén, K, Angerbjörn, A. 1999. Dietary change and stable isotopes: a model of growth and dormancy in cave bears. Proceedings of the Royal Society of London: Series B 266(1430):17791783.Google Scholar
Longinelli, A, Selmo, E. 2011. δ18O values of Sus scrofa blood water and bone phosphate; a marked discrepancy between domestic and wild specimens. Rapid Commun. Mass Spectrom. 25:37323734.CrossRefGoogle ScholarPubMed
Magafa, M. 2002. The archaeo-botanical study of the settlement, in Dispilio. In Chourmouziadis G, editor. Dispilio, 7500 Years After. Thessaloniki: University Studio Press. p 115134. In Greek.Google Scholar
Makarewicz, CA. 2014. Winter pasturing practices and variable fodder provisioning detected in nitrogen (δ15N) and carbon (δ13C) isotopes in sheep dentinal collagen. J. Archaeol. Sci. 41:502510.Google Scholar
Makarewicz, C, Tuross, N. 2006. Foddering by Mongolian pastoralists is recorded in the stable carbon (δ13C) and nitrogen (δ15N) isotopes of caprine dentinal collagen. J. Archaeol. Sci. 33:862870.Google Scholar
Melfos, V, Stratoulis, G. 2002. The Dispilio excavations. In: Chourmouziadis G, editor. Dispilio, 7500 Years After. Thessaloniki: University Studio Press. In Greek.Google Scholar
Minagawa, M, Wada, E. 1984. Stepwise enrichment of 15N along food chains: further evidence and the relation between δ15N and animal age. Geochim. Cosmochim. Acta 48:11351140.Google Scholar
Ntinou, M. 2002. La paleovegetación en el Norte de Grecia desde el Tardiglaciar hasta el Atlántico. Formaciones Vegetales, Recursos y Usos. Oxford: British Archaeological Reports, International Series.Google Scholar
Noe-Nygaard, N, Price, TD, Ulfeldt Hede, S. 2005. Diet of aurochs and early cattle in southern Scandinavia: evidence from 15N and 13C stable isotopes. J. Archaeol. Sci. 32:855872.CrossRefGoogle Scholar
Oelze, VM, Siebert, A, Nicklisch, N, Meller, H, Dresely, V, Alt, KW. 2011. Early Neolithic diet and animal husbandry: stable isotope evidence from three Linearbandkeramik (LBK) sites in Central Germany. J. Archaeol. Sci. 38(2):270279.Google Scholar
Papathanasiou, A, Richards, MP. 2015. Summary: patterns in the carbon and nitrogen isotope data through time. In: Papathanasiou A., Richards M.P., Fox S.C., editors. Archaeodiet in the Greek World: Dietary Reconstruction from Stable Isotope Analysis. Athens: The American School of Classical Studies.Google Scholar
Phoca-Cosmetatou, N. 2008. The terrestrial economy of a lake settlement: a preliminary report on the faunal assemblage from the first phase of occupation of Dispilio (Kastoria, Greece). Anaskamma 2:4767.Google Scholar
Post, DM. 2002. Using stable isotopes to estimate trophic position: models, methods, and assumptions. Ecology 83:703718.Google Scholar
Rehren, T, Connolly, P, Schibille, N, Schwarzer, H. 2015. Changes in glass consumption in Pergamon (Turkey) from Hellenistic to late Byzantine and Islamic times. J. Archaeol. Sci. 55:266279.CrossRefGoogle Scholar
Reitsema, LJ. 2013. Beyond diet reconstruction: stable isotope applications to human physiology, health and nutrition. Am. J. Hum. Biol. 25:445456.Google Scholar
Richards, MP, Trinkaus, E. 2009. Isotopic evidence for the diets of European Neanderthals and early modern humans. Proc. Natl. Acad. Sci 106(38):1603416039.Google Scholar
Rindos, D. 1984. The Origins of Agriculture: An Evolutional Approach. Orlando: Academic Press.Google Scholar
Samartzidou, E. 2012. Preliminary remarks on the faunal assemblage of the unique Neolithic lakeside settlement of Greece: Dispilio (prefecture of Kastoria). In: Léfèvre C, editor. Proceedings of the General Session of the 11th International Council for Archaeozoology Conference (Paris, 23–28 August 2010). BAR International Series 2354: 137–46.Google Scholar
Samartzidou, E. 2014. Faunal assemblages in lakeside settlements of Neolithic Europe: the case of the lakeside settlement of Dispilio Kastorias (Greece, Western Macedonia) [PhD thesis]. School of History and Archaeology, Aristotle University Thessaloniki. 618 p.Google Scholar
Schoeninger, MJ, DeNiro, MJ. 1984. Nitrogen and carbon isotopic composition of bone collagen from marine and terrestrial animals. Geochim. Cosmochim. Acta 48:625639.Google Scholar
Schley, L, Roper., TJ. 2003. Diet of wild boar Sus scrofa in Western Europe, with particular reference to consumption of agricultural crops. Mammal Review 33:4356.Google Scholar
Schwarcz, PH, Dupras, LT, Fairgrieve, IS. 1999. 15N Enrichment in the Sahara: in search of a global relationship. Journal of Archaeological Science 26:629636.Google Scholar
Sealy, JC, Van der Merwe, NJ, Lee-Thorp, JA, Lanham, JL. 1987. Nitrogen isotopic ecology in southern Africa: implications for environmental and dietary tracing. Geochim. Cosmochim. Acta 51:27072717.CrossRefGoogle Scholar
Sofronidou, M. 2008. The prehistoric lakeside settlement of Dispilio, Kastoria: a first introduction. Anaskamma 1:926.Google Scholar
Sponheimer, M, Robinson, T, Ayliffe, L, Roeder, B, Hammer, J, Passey, B, West, A, Cerling, T, Dearing, D, Ehleringer, J. 2003a. Nitrogen isotopes in mammalian herbivores: hair δ15N values from a controlled feeding study. Int. J. Osteoarchaeol 13:8087.Google Scholar
Sponheimer, M, Robinson, T, Ayliffe, L, Passey, B, Roeder, B, Shipley, L, Lopez, E, Cerling, T, Dearing, D, Ehleringer, J. 2003b. An experimental study of carbon isotope fractionation between diet, hair, and feces of mammalian herbivores. Can. J. Zool. 81:871876.Google Scholar
Szpak, P. 2014. Complexities of nitrogen isotope biogeochemistry in plant-soil systems: implications for the study of ancient agricultural and animal management practices. Front. Plant Sci. 5:288.Google Scholar
Talbot, MR, Lærdal, T. 2000. The late Pleistocene Holocene of Lake Victoria, East Africa, based upon elemental and isotopic analyses of sedimentary organic matter. J. Paleoclimat 23:141164.Google Scholar
Touloumis, K. 2002. The economy of a Neolithic lakeside settlement, in Dispilio. In: Chourmouziadis G, editor. Dispilio, 7500 Years After. Thessaloniki: University Studio Press. In Greek.Google Scholar
Tselika, V. 2006. The form and evolution of prehistoric settlements in Greece: spatial framework and urban planning [PhD thesis]. School of Architecture, Department of Spatial Planning and Development, Aristotle University of Thessaloniki.Google Scholar
Vanderklift, MA, Ponsard, S. 2003. Sources of variation in consumer-diet δ15N enrichment: a meta-analysis. Oecologia 136:169182.Google Scholar
Whiticar, MJ. 1999. Carbon and hydrogen isotope systematic of bacterial formation and oxidation of methane. Chem Geol 161:291314.Google Scholar
Wittwer-Backofen, U, Tomo, N. 2008. From health to civilization stress? In search for traces of a health transition during the early Neolithic in Europe. In: Bocquet-Appel J-P, Bar-Yosef O, editors. The Neolithic Demographic Transition and its Consequences. New York: Springer. p 501538.Google Scholar