Hostname: page-component-745bb68f8f-kw2vx Total loading time: 0 Render date: 2025-01-10T20:49:24.087Z Has data issue: false hasContentIssue false
  • English
  • Français

Hydroxyproline Dating: Experiments on the 14C Analysis of Contaminated and Low-Collagen Bones

Published online by Cambridge University Press:  09 February 2016

Anat Marom ,
James S O McCullagh ,
Thomas F G Higham  and
Robert E M Hedges
Anat Marom
Affiliation:
Research Laboratory for Archaeology and the History of Art, University of Oxford, Oxford OX1 3QY, United Kingdom
James S O McCullagh
Affiliation:
Chemistry Research Laboratory, University of Oxford, Oxford OX1 3TA, United Kingdom
Thomas F G Higham
Affiliation:
Research Laboratory for Archaeology and the History of Art, University of Oxford, Oxford OX1 3QY, United Kingdom
Robert E M Hedges
Affiliation:
Research Laboratory for Archaeology and the History of Art, University of Oxford, Oxford OX1 3QY, United Kingdom
Get access Rights & Permissions [Opens in a new window]

Abstract

Dating of the amino acid hydroxyproline from bone collagen has been shown to produce accurate and reliable radiocarbon dates. This article presents further application of the method demonstrating it can be used to obtain dates for both low-collagen and contaminated bones, extending the capability of 14C dating archaeological bone from conventional limits imposed by alternative pretreatment methods. The method therefore has the potential for significantly benefiting the accelerator mass spectrometry (AMS) dating community in the 14C dating of archaeological bone.

Type
Articles
Information
Radiocarbon , Volume 55 , Issue 2: Proceedings of the 21st International Radiocarbon Conference (Part 1 of 2) , 2013 , pp. 698 - 708
Copyright
Copyright © 2013 by the Arizona Board of Regents on behalf of the University of Arizona 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

2

Current address: Kimmel Centre for Archaeological Science, Weizmann Institute of Sciences, POB 26, Rehovot 76100, Israel.

References

Abelson, PH, Hoering, TC. 1961. Carbon isotope fractionation in formation of amino acids by photosynthetic organisms. Proceedings of the National Academy of Sciences of the USA 47(5):623–32.Google Scholar
Ambrose, SH. 1990. Preparation and characterization of bone and tooth collagen for isotopic analysis. Journal of Archaeological Science 17(4):431–51.Google Scholar
Brock, F, Bronk Ramsey, C, Higham, T. 2007. Quality assurance of ultrafiltered bone dating. Radiocarbon 49(2): 187–92.Google Scholar
Brock, F, Higham, T, Ditchfield, P, Bronk Ramsey, C. 2010a. Current pretreatment methods for AMS radiocarbon dating at the Oxford Radiocarbon Accelerator Unit (ORAU). Radiocarbon 52(1): 103–12.Google Scholar
Brock, F, Higham, T, Bronk Ramsey, C. 2010b. Pre-screening techniques for identification of samples suitable for radiocarbon dating of poorly preserved bones. Journal of Archaeological Science 37(4):855–65.Google Scholar
Bronk Ramsey, C. 2009. Bayesian analysis of radiocarbon dates. Radiocarbon 51(1):337–60.Google Scholar
Clark, G. 1954. Excavations at Star Carr: An Early Mesolithic Site at Seamer near Scarborough, Yorkshire. New York: Cambridge University Press.Google Scholar
Cloutman, EW. 1988. Palaeoenvironments in the Vale of Pickering part 1 : stratigraphy and palaeogeography of Seamer Carr, Star Carr and Flixton Carr. Proceedings of the Prehistoric Society 54:1–19.Google Scholar
Conneller, C, Milner, N, Taylor, B, Taylor, M. 2012. Substantial settlement in the European Early Mesolithic: new work at Star Carr. Antiquity 86(334): 1004–20.Google Scholar
Dark, P, Higham, T, Jacobi, R, Lord, T. 2006. New radiocarbon accelerator dates on artefacts from the Early Mesolithic site of Star Carr, North Yorkshire. Archaeometry 48(1):185–200.Google Scholar
De Vivo, B, Rolandi, G, Gans, PB, Calvert, A, Bohrson, WA, Spera, FJ, Belkin, HE. 2001. New constraints on the pyroclastic eruptive history of the Campanian volcanic Plain (Italy). Mineralogy and Petrology 73(1):47–65.Google Scholar
Douka, K, Higham, T, Sinitsyn, A. 2010. The influence of pretreatment chemistry on the radiocarbon dating of Campanian Ignimbrite-aged charcoal from Kostenki 14 (Russia). Quaternary Research 73(3):583–7.CrossRefGoogle Scholar
Fiedel, SJ, Southon, JR, Taylor, RE, Kuzmin, YV, Street, M, Higham, TFG, van der Plicht, J, Nadeau, M-J, Chavan, S, 2013. Assessment of interlaboratory pretreatment protocols by radiocarbon dating an elk bone found below Laacher See tephra at Miesenheim IV (Rhineland, Germany). Radiocarbon, these proceedings, doi: 10.2458/azu_js_rc.55.16239.CrossRefGoogle Scholar
Gillespie, R, Hedges, REM, Wand, JO. 1984. Radiocarbon dating of bone by accelerator mass spectrometry. Journal of Archaeological Science 11(2): 165–70.Google Scholar
Gillespie, R, Hedges, REM, Humm, MJ. 1986. Routine AMS dating of bone and shell proteins. Radiocarbon 28(2A):451–6.Google Scholar
Haesaerts, P, Damblon, F, Sinitsyn, A, van der Plicht, J. 2004. Kostenki 14 (Voronezh, central Russia): new data on stratigraphy and radiocarbon chronology. Acts of the XIVth UISPP Congress. University of Liege, Belgium. BAR International Series. p 169–80.Google Scholar
Hare, PE. 1980. Organic geochemistry of bone and its relation to the survival of bone in the natural environment. In: Behrensmeyer, AK, Hill, AP, editors. Fossils in the Making: Vertebrate Taphonomy and Paleoecology. Chicago: University of Chicago Press. p 208–19.Google Scholar
Hedges, REM, Law, IA. 1989. The radiocarbon dating of bone. Applied Geochemistry 4:249–53.Google Scholar
Higham, T. 2011. European Middle and Upper Palaeolithic radiocarbon dates are often older than they look: problems with previous dates and some remedies. Antiquity 85(327):235–49.CrossRefGoogle Scholar
Ho, TY, Marcus, LF, Berger, R. 1969. radiocarbon dating of petroleum-impregnated bone from tar pits at Rancho La Brea, California. Science 164(3883): 1051–2.CrossRefGoogle ScholarPubMed
Hoffecker, JF, Holliday, VT, Anikovich, MV, Sinitsyn, AA, Popov, VV, Lisitsyn, SN, Levkovskaya, GM, Pospelova, GA, Forman, SL, Giaccio, B. 2008. From the Bay of Naples to the River Don: the Campanian Ignimbrite eruption and the Middle to Upper Paleolithic transition in Eastern Europe. Journal of Human Evolution 55(5):858–70.CrossRefGoogle Scholar
Marom, A. 2012. Development and application of an analytical method for radiocarbon dating bone using the amino acid hydroxyproline , xford: University of Oxford.Google Scholar
Marom, A, McCullagh, JSO, Higham, TFG, Sinitsyn, AA, Hedges, REM. 2012. Single amino acid radiocarbon dating of Upper Paleolithic modern humans. Proceedings of the National Academy of Sciences of the USA 109(18):6878–81.CrossRefGoogle ScholarPubMed
McCullagh, JSO, Marom, A, Hedges, REM. 2010. Radiocarbon dating of individual amino acids from archaeological bone collagen. Radiocarbon 52(2):620–34.Google Scholar
Nalawade-Chavan, S, McCullagh, J, Hedges, R, Bonsall, C, Boroneant, A, Bronk Ramsey, C, Higham, T. 2013. Compound specific radiocarbon dating of essential and non-essential amino acids: towards determination of dietary reservoir effects in humans. Radiocarbon, these proceedings, doi:10.2458/azu_js_rc.55.16340.Google Scholar
Reimer, PJ, Baillie, MGL, Bard, E, Bayliss, A, Beck, JW, Blackwell, PG, Bronk Ramsey, C, Buck, CE, Burr, GS, Edwards, RL, Friedrich, M, Grootes, PM, Guilderson, TP, Hajdas, I, Heaton, TJ, Hogg, AG, Hughen, KA, Kaiser, KF, Kromer, B, McCormac, FG, Manning, SW, Reimer, RW, Richards, DA, Southon, JR, Talamo, S, Turney, CSM, van der Plicht, J, Weyhenmeyer, CE. 2009. IntCal09 and Marine09 radiocarbon age calibration curves, 0–50,000 years cal BP. Radiocarbon 51(4): 1111–50.CrossRefGoogle Scholar
Schadla-Hall, R, Lane, TPJ. In press. Hunter-Gatherers in the Landscape: Archaeological and Paleoenvironmental Investigations in the Vale of Pickering, North Yorkshire 1976–2000. Chapter 10.1. Cambridge: McDonald Institute for Archaeological Research.Google Scholar
Stafford, TW Jr, Duhamel, RC, Haynes, CV Jr, Brendel, K. 1982. Isolation of proline and hydroxyproline from fossil bone. Life Sciences 31:931–8.Google Scholar
Stafford, TW Jr, Brendel, K, Duhamel, RC. 1988. Radiocarbon, 13C and 15N analysis of fossil bone: removal of humates with XAD-2 resin. Geochimica et Cosmochimica Acta 52(9):2257–67.CrossRefGoogle Scholar
Stafford, TW Jr, Hare, PE, Currie, L, Jull, AJT, Donahue, DJ. 1991. Accelerator radiocarbon dating at the molecular level. Journal of Archaeological Science 18(1):35–72.Google Scholar
van Klinken, GJ. 1999. Bone collagen quality indicators for palaeodietary and radiocarbon measurements. Journal of Archaeological Science 26(6):687–95.Google Scholar
van Klinken, GJ, Hedges, REM. 1995. Experiments on collagen-humic interactions: speed of humic uptake, and effects of diverse chemical treatments. Journal of Archaeological Science 22(2):263–70.Google Scholar
van Klinken, GJ, Mook, WG. 1990. Preparative high performance liquid chromatographic separation of individual amino acids derived from fossil bone collagen. Radiocarbon 32(2): 155–64.Google Scholar
van Klinken, GJ, Bowels, AD, Hedges, REM. 1994. Radiocarbon dating of peptides isolated from contaminated fossil bone collagen by collagenase digestion and reverse-phase chromatography. Geochimica et Cosmochimica Acta 58(11):2453–551.Google Scholar