Developing cells have constantly to make decisions: when to proliferate and divide, when and how to differentiate. It is an increasingly attractive idea that these decisions involve changes in intracellular cation concentrations. Our ideas about the mechanisms of changes in intracellular cations come largely from the application of biophysical techniques in the study of excitable tissues. These ideas are proving very valuable to the investigation of the control of proliferation and cell development and it is evident that the ionic mechanisms which pertain in nerve and muscle have their counterparts in other cells. Just as alterations in intracellular ion concentrations serve a signalling function in excitable tissue, so too they act as signals during development. Since almost all the quantitative data on the ionic mechanisms of fertilization come from work on sea urchins we have confined our review to sea urchin eggs.

This review represents a personal view of membrane thermodynamics. I do not intend to deal at all with the irreversible thermodynamics of membrane mass transfer processes. This aspect has been covered far more competently and completely by other people (Bittar, 1970; Paterson, 1970; Rottenberg, Caplan & Essig, 1970; Mitchell, 1970; Rothschild et al. 1980; Oster, Perelson & Katchalsky, 1973; Kedem & Katchalsky, 1958; Schwartz, 1971). The recent review on osmosis by Hill (1979) is a particularly succinct appraisal of a facet of irreversible membrane thermodynamics. Arata & Nishimura (1980) have considered the coupling of electron transfer to vectorial processes in biological membranes.