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Luminescence spectroscopic approaches in studying cell surface dynamics

Published online by Cambridge University Press:  17 March 2009

Janos Matkó ,
Janos Szöllösi ,
Lajos Trón  and
Sandor Damjanovich
Janos Matkó
Affiliation:
Department of Biophysics, Medical University of Debrecen, H-4012 Debrecen, POB 3, Hungary
Janos Szöllösi
Affiliation:
Department of Biophysics, Medical University of Debrecen, H-4012 Debrecen, POB 3, Hungary
Lajos Trón
Affiliation:
Department of Biophysics, Medical University of Debrecen, H-4012 Debrecen, POB 3, Hungary
Sandor Damjanovich
Affiliation:
Department of Biophysics, Medical University of Debrecen, H-4012 Debrecen, POB 3, Hungary
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The major elements of membranes, such as proteins, lipids and polysaccharides, are in dynamic interaction with each other (Alberts et al. 1983). Protein diffusion in the lipid matrix of the membrane, the lipid diffusion and dynamic domain formation below and above their transition temperature from gel to fluid state, have many functional implications. This type of behaviour of membranes is often summarized in one frequently used word membrane fluidity (coined by Shinitzky & Henkart, 1979). The dynamic behaviour of the cell membrane includes rotational, translational and segmental movements of membrane elements (or their domain-like associations) in the plane of, and perpendicular to the membrane. The ever changing proximity relationships form a dynamic pattern of lipids, proteins and saccharide moieties and are usually described as ‘cell-surface dynamics’ (Damjanovich et al. 1981). The knowledge about the above defined behaviour originates from experiments performed mostly on cytoplasmic membranes of eukaryotic cells. Nevertheless numerous data are available also on the mitochondrial and nuclear membranes, as well as endo (sarco-)plasmic reticulum (Martonosi, 1982; Slater, 1981; Siekevitz, 1981).

Type
Research Article
Information
Quarterly Reviews of Biophysics , Volume 21 , Issue 4 , November 1988 , pp. 479 - 544
Copyright
Copyright © Cambridge University Press 1988

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