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Hydrodynamic properties of complex, rigid, biological macromolecules: theory and applications

Published online by Cambridge University Press:  17 March 2009

José García de la Torre  and
Victor A. Bloomfield
José García de la Torre
Affiliation:
Departamento de Qulmica-FIsica, Faculdad de Ciencias, Universidad de Extremadura, Badajoz, Spain
Victor A. Bloomfield
Affiliation:
Department of Biochemistry, University of Minnesota, St Paul, Minnesota 55108†
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Extract

Among the Various methods for characterizing macromolecules in solution, hydrodynamic techniques play a major role. Since the advent of the ultracentrifuge and the development of viscometric apparatus, sedimentation coefficients and intrinsic viscosities have been extensively used to learn about the size and shape of synthetic and biological polymers. More recently, refined techniques such as quasielastic light scattering, transient electric birefringence and fluorescence anisotropy decay have made it possible to obtain in a simple and rapid way quantitative information of high precision on the translational and rotational brownian dynamics of dissolved macromolecules.

Type
Research Article
Information
Quarterly Reviews of Biophysics , Volume 14 , Issue 1 , February 1981 , pp. 81 - 139
Copyright
Copyright © Cambridge University Press 1981

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