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Determination of the Mass of Viruses by Quantitative Electron Microscopy

Published online by Cambridge University Press:  17 March 2009

G. F. Bahr ,
W. F. Engler  and
H. M. Mazzone
G. F. Bahr
Affiliation:
Department of Cellular Pathology, Armed Forces Institute of Pathology, Washington, D.C., U.S.A.
W. F. Engler
Affiliation:
Department of Cellular Pathology, Armed Forces Institute of Pathology, Washington, D.C., U.S.A.
H. M. Mazzone
Affiliation:
Forest Insect and Disease Laboratory, Forest Service, U.S. Department of Agriculture, Hamden, Conn., U.S.A.
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Extract

In general the characterization of a virus particle depends upon a number of known and independently acquired parameters, all of which require a great deal of time. As a direct method of measurement, the mass of viruses has been largely ignored in virus characterization studies. Where the molecular weight of viruses has been determined by sedimentation and diffusion, sedimentation equilibrium, light scattering, or the electron microscopy-counting procedure, the relationship between this property and mass has been arrived at by employing Avogadro's number. In the present report it will be demonstrated that the disadvantages found in these usual methods of determining molecular weight, such as experimentation, calculation of virus size, and purification of virus preparation, are not present in the quantitative electron microscopy method for determination of mass and molecular weight.

Type
Research Article
Information
Quarterly Reviews of Biophysics , Volume 9 , Issue 4 , November 1976 , pp. 459 - 489
Copyright
Copyright © Cambridge University Press 1976

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References

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