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The application of the molecular replacement method to the de novo determination of protein structure

Published online by Cambridge University Press:  17 March 2009

Michael C. Lawrence
Michael C. Lawrence
Affiliation:
CSIRO Division of Biomolecular Engineering, 343 Royal Parade, Parkville, Victoria 3052, Australia
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Extract

The determination of a novel protein structure by X-ray diffraction is seldom straightforward. Three hurdles present themselves (i) the protein must be purified in sufficient quantity to allow crystallization trials, (ii) crystals must be grown to adequate size and must diffract to a resolution that will allow atomic detail to be revealed, and (iii) phases must be determined for the diffracted X-ray beams in order that an initial electron-density map may be calculated.

Type
Research Article
Information
Quarterly Reviews of Biophysics , Volume 24 , Issue 4 , November 1991 , pp. 399 - 424
Copyright
Copyright © Cambridge University Press 1991

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