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The interpretation of tracer fluxes in terms of membrane structure*

Published online by Cambridge University Press:  17 March 2009

Hans H. Ussing
Hans H. Ussing
Affiliation:
Institute of Biological Chemistry, University of Copenhagen
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Extract

Very early in the era of isotopic tracers it became apparent that tracer fluxes did not always come out as one had predicted from classical measurements of transport by osmosis or diffusion. Thus Hevesy, Hofer & Krogh (1935) determined the water permeability of frog skin from osmotic uptake of water as well as from measurements of its permeability to heavy water. They found the first method to give values some 4–5 times higher than the latter. Although this result at first seemed disappointing, it was soon realized that the two types of experiments provided more information about the nature of osmosis through real membranes than had been available before. Thus it was shown that the results might be explained in terms of interaction between water molecules moving in bulk, possibly through pores in the membrane (Ussing, 1952; Koefoed-Johnsen & Ussing, 1953; Andersen & Ussing, 1957).

Type
Research Article
Information
Quarterly Reviews of Biophysics , Volume 1 , Issue 4 , January 1969 , pp. 365 - 376
Copyright
Copyright © Cambridge University Press 1969

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References

REFERENCES

Andersen, B. & Ussing, H. H. (1957). Solvent drag on non-electrolytes during osmotic flow through isolated toad skin and its response to antidiuretic hormone. Acta physiol. Scand. 39, 228–39.CrossRefGoogle ScholarPubMed
Biber, T. U. L. & Curran, P. F. (1968). Coupled solute fluxes in toad skin. J. gen. Physiol. 51, 606–20.CrossRefGoogle ScholarPubMed
Farquhar, M. G. & Palade, G. E. (1964). Functional organization of amphibian skin. Proc. natn. Acad. Sci. U.S.A. 51, 569–77.CrossRefGoogle ScholarPubMed
Franz, T. J. & Van Bruggen, J. T. (1967). Hyperosmolarity and the net transport of nonelectrolytes in frog skin. J. gen. Physiol. 50, 933–49.CrossRefGoogle ScholarPubMed
Hevesy, V. G., Hofer, E. & Krogh, A. (1935). The permeability of the skin of frogs to water as determined by D2O and H2O. Scand. Arch. Physiol. 72, 199–214.CrossRefGoogle Scholar
Johnsen, V. K., Levi, H. & Ussing, H. H. (1952). The mode of passage of chloride ions through the isolated frog skin. Acta physiol. Scand. 25, 150–63.CrossRefGoogle ScholarPubMed
Koefoed-Johnsen, V. & Ussing, H. H. (1953). The contribution of diffusion and flow to the passage of D2O through living membranes. Acta physiol. Scand. 28, 60–76.CrossRefGoogle Scholar
Koefoed-johnsen, V. & Ussing, H. H. (1958). The nature of the frog skin potential. Acta physiol. Scand., 42, 298–303.CrossRefGoogle ScholarPubMed
Lassen, N. A., Munck, O. & Thaysen, J. H. (1961). Oxygen consumption and sodium reabsorption in the kidneys. Acta physiol. Scand. 51, 371–84.CrossRefGoogle Scholar
Kramer, K. & Deetjen, P. (1960). Beziehungen des O2-Verbrauchs der Niere zu Durchblutung und Glomerulusfiltrat bei Änderung des arteriellen Druckes. Pflügers Arch. ges. Physiol. 271, 782–96.CrossRefGoogle Scholar
MacRobbie, E. A. C. & Ussing, H. H. (1961). Osmotic behaviour of the epithelial cells of frog skin. Acta physiol. Scand. 53, 348–65.CrossRefGoogle ScholarPubMed
Ogilvie, J. T., McIntosh, J. R. & Curran, P. F. (1963). Volume flow in a series-membrane system. Biochim. biophys. Acta 66, 441–4.Google Scholar
Schlögl, R. (1964). Stofftransport durch Membranen. Darmstadt: Dr Dietrich Steinkopf Verlag.Google Scholar
Ussing, H. H. (1949 a). The active ion transport through the isolated frog skin in the light of tracer studies. Acta physiol. Scand. 17, 1–37.CrossRefGoogle ScholarPubMed
Ussing, H. H. (1949b). The distinction by means of tracers between active transport and diffusion. Acta physiol. Scand. 19, 43–56.CrossRefGoogle Scholar
Ussing, H. H. (1952). Some aspects of the application of tracers in permeability studies. Adv. Enzymol. 13, 21–65.Google ScholarPubMed
Ussing, H. H. (1965). Relationship between osmotic reactions and active sodium transport in the frog skin epithelium. Acta physiol. Scand. 63, 141–55.CrossRefGoogle ScholarPubMed
Ussing, H. H. (1966). Anomalous transport of electrolytes and sucrose through the isolated frog skin induced by hypertonicity of the outside bathing solution. Ann. N.Y. Acad. Sci. 137, 543–55.CrossRefGoogle ScholarPubMed
Ussing, H. H. (1967). Active sodium transport across the frog skin epithelium and its relation to epithelial structure. Berichte d. Bunsenges. f. physik. Chemie 71, 807–13.CrossRefGoogle Scholar
Ussing, H. H. & Andersen, B. (1956). The relation between solvent drag and active transport of ions. Proc. 3rd Int. Congr. Biochem., Brussels, 1955, pp. 434–40. New York: Academic Press.Google Scholar
Ussing, H. H. & Windhager, E. E. (1964). Nature of shunt path and active sodium transport path through frog skin epithelium. Acta physiol. Scand. 61, 484–504.Google ScholarPubMed
Ussing, H. H. & Zerahn, K. (1951). Active transport of sodium as the source of electric current in the short-circuited isolated frog skin. Acta physiol. Scand. 23, 110–27.CrossRefGoogle ScholarPubMed
Zerahn, K. (1955). Studies on the active transport of lithium in the isolated frog skin. Ada physiol. Scand. 33, 347–58.CrossRefGoogle ScholarPubMed
Zerahn, K. (1956). Oxygen consumption and active sodium transport in the isolated and short-circuited frog skin. Acta physiol. Scand. 36, 300–18.CrossRefGoogle ScholarPubMed