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Fluorescence correlation spectroscopy applied to rotational diffusion of macromolecules

Published online by Cambridge University Press:  17 March 2009

Måns Ehrenberg  and
Rudolf Rigler
Måns Ehrenberg
Affiliation:
Department of Medical Biophysics, Karolinska Institute, 10401 Stockholm 60, Sweden
Rudolf Rigler
Affiliation:
Department of Medical Biophysics, Karolinska Institute, 10401 Stockholm 60, Sweden
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Extract

A quantitative relationship between polarization properties of fluorescence light and molecular rotational diffusion was first derived by Perrin (1926). His results, which concerned spherical particles, have later been refined to the more complex rotational motion of asymmetric bodies (Memming, 1961; Chuang & Eisenthal, 1972; Ehrenberg & Rigler, 1972; Belford, Belford & Weber, 1972).

Type
Research Article
Information
Quarterly Reviews of Biophysics , Volume 9 , Issue 1 , February 1976 , pp. 69 - 81
Copyright
Copyright © Cambridge University Press 1976

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References

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