Hostname: page-component-cd9895bd7-dk4vv Total loading time: 0 Render date: 2024-12-23T02:17:34.669Z Has data issue: false hasContentIssue false
  • English
  • Français

Chemical Kinetics and Fluorescence Correlation Spectroscopy†

Published online by Cambridge University Press:  17 March 2009

D. Magde
D. Magde
Affiliation:
Department of Chemistry, University of California, San Diego, La Jolla, Calfornia 92093, U.S.A.
Get access Rights & Permissions [Opens in a new window]

Extract

The dynamics of macromolecules, the subject of this symposium, are most directly studied by simply looking through a microscope and observing the molecular motion. With a microscope, we can resolve the size and shape of large particles, as well as monitor dynamic motion. For smaller particles, particularly single macromolecules, we cannot resolve the size or shape; but it is still possible to observe the motion, if we can make the particles appear as bright points of light sprinkled dilutely over a dark background. Siedentopf & Zsigmondy (1903) demonstrated this fact with a device which came to be called the ultramicroscope.

Type
Research Article
Information
Quarterly Reviews of Biophysics , Volume 9 , Issue 1 , February 1976 , pp. 35 - 47
Copyright
Copyright © Cambridge University Press 1976

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Elson, E. & Magde, D. (1974). Fluorescence correlation spectroscopy. I. Conceptional basis and theory. Biopolymers 13, 1–27.CrossRefGoogle Scholar
Feher, G. & Weissman, M. (1973). Fluctuation spectroscopy: Determination of chemical reaction kinetics from the frequency spectrum of fluctuations. Proc. Natn. Acad. Sci. U.S.A. 70, 870–5.CrossRefGoogle ScholarPubMed
Magde, D., Elson, E. & Webb, W. W. (1972). Thermodynamic fluctuations in a reacting system – measurement by fluorescence correlation spectroscopy. Phys. Rev. Lett. 29, 705–8.CrossRefGoogle Scholar
Magde, D., Elson, E. & Webb, W. W. (1974). Fluorescence correlation spectroscopy. II. An experimental realization. Biopolymers 13, 29–61.CrossRefGoogle Scholar
Siedentopf, H. & Zsigmondy, R. (1903). Über sichtbermachungund grössenbestimmung ultramikroskopischer Teilchen mit besonderer Anwendung auf Goldrubingläser. Annln Phys. 10, 1–39.Google Scholar
Svedberg, T. (1928). Colloid Chemistry, 2nd ed.New York: Chemical Catalogue Company.Google Scholar
Zingsheim, H. P. & Neher, E. (1974). The equivalence of fluctuation analysis chemical relaxation measurements: a kinetic study of ion pore formation in thin lipid membranes. Biophys. Chem. 2, 197–207.CrossRefGoogle ScholarPubMed