Previous studies have suggested that the memory underlying the mismatch negativity (MMN) can be deactivated by a period of silence and later reactivated. An alternative is that the memory becomes inapplicable due to a period of silence and subsequently reinstated. Both interpretations rest on the absence of a MMN for a deviant in Position 1 of a train (showing deactivation or inapplicability of the memory) and the presence of a MMN in Position 2 of the train (evidencing reactivation or reinstatement of the memory). In these studies, the standards were fixed across all trains of a given condition. Hence, it is not clear if the MMN elicited in Position 2 requires presentation of multiple trains with identical standards or only a single train. Experiment 1 showed that a single train is sufficient. With data from recent studies, Experiment 2 showed that MMNs in Position 2 of trains are due to reinstatement rather than reactivation of the memory.