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Early intermediates in the PDI-assisted folding of ribonuclease A

Published online by Cambridge University Press:  01 March 2000

FLORIANA VINCI
Affiliation:
Centro Internazionale di Servizi Spettrometria di Massa Via Pansini, 5 80131 Napoli, Italy Dipartimento di Chimica Organica e Biologica Università degli Studi di Napoli, Napoli, Italy
MARGHERITA RUOPPOLO
Affiliation:
Dipartimento di Chimica Università degli Studi di Salerno, Salerno, Italy Centro Internazionale di Servizi Spettrometria di Massa Via Pansini, 5 80131 Napoli, Italy
PIERO PUCCI
Affiliation:
Centro Internazionale di Servizi Spettrometria di Massa Via Pansini, 5 80131 Napoli, Italy Dipartimento di Chimica Organica e Biologica Università degli Studi di Napoli, Napoli, Italy CEINGE, Biotecnologie Avanzate, scrl, Naples, Italy
ROBERT B. FREEDMAN
Affiliation:
Research School of Biosciences, Biological Laboratory, University of Kent, Canterbury CT2 7NJ, United Kingdom
GENNARO MARINO
Affiliation:
Centro Internazionale di Servizi Spettrometria di Massa Via Pansini, 5 80131 Napoli, Italy Dipartimento di Chimica Organica e Biologica Università degli Studi di Napoli, Napoli, Italy CEINGE, Biotecnologie Avanzate, scrl, Naples, Italy
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Abstract

The oxidative refolding of ribonuclease A has been investigated in several experimental conditions using a variety of redox systems. All these studies agree that the formation of disulfide bonds during the process occurs through a nonrandom mechanism with a preferential coupling of certain cysteine residues. We have previously demonstrated that in the presence of glutathione the refolding process occurs through the reiteration of two sequential reactions: a mixed disulfide with glutathione is produced first which evolves to form an intramolecular S-S bond. In the same experimental conditions, protein disulfide isomerase (PDI) was shown to catalyze formation and reduction of mixed disulfides with glutathione as well as formation of intramolecular S-S bonds.

This paper reports the structural characterization of the one-disulfide intermediate population during the oxidative refolding of Ribonuclease A under the presence of PDI and glutathione with the aim of defining the role of the enzyme at the early stages of the reaction. The one-disulfide intermediate population occurring at the early stages of both the uncatalyzed and the PDI-catalyzed refolding was purified and structurally characterized by proteolytic digestion followed by MALDI-MS and LC/ESIMS analyses. In the uncatalyzed refolding, a total of 12 disulfide bonds out of the 28 theoretical possible cysteine couplings was observed, confirming a nonrandom distribution of native and nonnative disulfide bonds. Under the presence of PDI, only two additional nonnative disulfides were detected. Semiquantitative LC/ESIMS analysis of the distribution of the S-S bridged peptides showed that the most abundant species were equally populated in both the uncatalyzed and the catalyzed process.

This paper shows the first structural characterization of the one-disulfide intermediate population formed transiently during the refolding of ribonuclease A in quasi-physiological conditions that mimic those present in the ER lumen. At the early stages of the process, three of the four native disulfides are detected, whereas the Cys26–Cys84 pairing is absent. Most of the nonnative disulfide bonds identified are formed by nearest-neighboring cysteines. The presence of PDI does not significantly alter the distribution of S-S bonds, suggesting that the ensemble of single-disulfide species is formed under thermodynamic control.

Type
Research Article
Copyright
© 2000 The Protein Society

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