Hostname: page-component-78c5997874-94fs2 Total loading time: 0 Render date: 2024-11-10T01:37:33.323Z Has data issue: false hasContentIssue false
  • English
  • Français

V.—Investigations into the Distribution of Thiol Groups in the Skin Follicles of Mice and Sheep and the Entry of Labelled Sulphur Compounds

Published online by Cambridge University Press:  11 June 2012

M. L. Ryder
M. L. Ryder
Affiliation:
Biology Department, Wool Industries Research Association.
Get access

Synopsis

The distribution of thiol (sulphydryl) groups in the wool follicle has been studied with Bennett's reagent; the strongest reaction was found immediately proximal to the prekeratinization (keratogenous) region. Mice and lambs were injected with cystine labelled with sulphur-35 and radioactivity was detected in the follicles, autoradiographically within a few minutes. A peak of activity formed in the follicle during the first few hours after injection which suggested that there is a rapid turnover of cystine in the body. But a small amount of activity could still be detected in the follicle up to at least 3 weeks afterwards.

Sulphur seems to enter the follicle immediately above the bulb, and reaches the fibre first on the side at which it is keratinized last. This method indicated the relative proportion of sulphur in different regions of the follicle and suggested that the sulphur becomes concentrated on keratinization. No difference in sulphur content was found between primary and secondary follicles, the ortho- and paracortex, and apparently between cortex and medulla. When labelled sulphate was injected, activity appeared first in the outer sheath, and only later in the fibre.

Type
Research Article
Information
Proceedings of the Royal Society of Edinburgh, Section B: Biological Sciences , Volume 67 , Issue 1 , 1958 , pp. 65 - 82
Copyright
Copyright © Royal Society of Edinburgh 1958

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

References to Literature

Auber, L., 1952. “The Anatomy of Follicles producing Wool Fibres, with Special Reference to Keratinization”, Trans. Roy. Soc. Edin., 62, 191–254.CrossRefGoogle Scholar
Auber, L., and Ryder, M. L., 1956. “Anomalies in Structure and Development of Wool Fibres”, Proc. Int. Wool Text. Res. Conf. Aust., 1955, F, 36–62.Google Scholar
Bennett, H. S., 1951. “The Demonstration of Thiol Groups in Certain Tissues by Means of a Coloured Sulphydryl Reagent”, Anat. Rec., 110, 231–247.CrossRefGoogle Scholar
Bern, H. A., 1954. “Histology and Chemistry of Keratin Formation”, Nature, Lond., 174, 509–511.CrossRefGoogle ScholarPubMed
Bern, H. A., Harkness, D. R., and Blair, S. M., 1955. “Radioautographic Studies of Keratin Formation”, Proc. Nat. Acad. Sci. Wash., 41, 55–60.CrossRefGoogle ScholarPubMed
Blackburn, S., 1948. “The Composition and Reactivity of Medullated Keratins”, Biochem. J., 43, 114–117.Google Scholar
Carter, H. B., 1943. “Studies in the Biology of the Skin and Fleece of Sheep”, Bull. Coun. Sci. Industr. Res. Aust., No. 164.Google Scholar
Doniach, I., and Pelc, S. R., 1950. “Autoradiographic Technique”, Br. J. Radiol., 23, 184–192.CrossRefGoogle Scholar
Dry, F. W., Burley, R. W., and Speakman, J. B., 1952. “Plasticity of Wool”, Nature, Lond., 169, 371Google Scholar
Frasery, R. D. B., and Rogers, G. E., 1955. “The Bilateral Structure of Wool Cortex and its Relation to Crimp”, Aust. J. Biol. Sci., 8, 288–299.Google Scholar
Giroud, A., Bulliard, H., and Giberton, A., 1929. “Y-a-t'il Une Accumulation Elective de Soufre Precedant la Keratinization”?, C.R. Soc. Biol. Paris, 101, 1024.Google Scholar
Giroud, A., Bulliard, H., 1930. “La Keratinization de l'Epiderme et des Phaneres. Genese des Substances Soufrees de la Keratines”, Arch. Morph. Gen. Exp., fasc. 29, 1.Google Scholar
Giroud, A., and Leblond, C. P., 1951. “The Keratinization of Epidermis and its Derivatives, especially the Hair as shown by X-ray Diffraction and Histological Studies”, Ann. N.Y. Acad. Sci., 53, 613–626.Google Scholar
Hale, W. H., and Garrigus, U. S., 1953. “Synthesis of Cystine in Wool from Elemental Sulphur and Sulphate Sulphur”, J. Anim. Set., 12, 492.Google Scholar
Hardy, M. H., 1952. “The Histochemistry of Hair Follicles in the Mouse”, Amer.J. Anat., 90, 285–338.Google Scholar
Horio, M., and Kondo, T., 1953. “Crimping of Wool Fibres”, Text. Res. (J.), 23, 373–387.Google Scholar
Mercer, E. H., Golden, R. L., and Jeffries, E. B., 1954. “Distribution of Cystine in the Cortex of Wool”, Text. Res. (J.), 24, 615–618.Google Scholar
Mescon, H., and Flesch, P., 1952. “Modification of Bennett's Method for Histochemical Demonstration of Free SH Groups in Skin”, J. Invest. Derm., 18, 261–266.Google Scholar
Montagna, W., Eisen, A. Z., Rademacher Ann, H., and Chase, H. B., 1954. “Histological and Cytochemical Studies of Human Skin. VI. The Distribution of Sulphydryl and Disulphide Groups”, J. Invest. Derm. 23, 23–32.Google Scholar
Pearse, A. G. E., 1953. Histochemistry Theoretical and Applied. Churchill, London.Google Scholar
Pelc, S. R., and Glucksmann, A., 1955. “Sulphate Metabolism in the Cartilage of the Trachea, Pinna and Xyphoid Process of the Adult Mouse as Indicated by Autoradiographs “, Exp. Cell. Res., 8, 336–344.Google Scholar
Rothman, S., 1954. Physiology and Biochemistry of the Skin. Chicago University Press.Google Scholar
Rothman, S., and Schaaf, F., 1929. “Chemie der Haut”, In: Jadassohn, Handb.d., Haut-U Geschlechtskr. 1/2, 161. J. Springer, Berlin.Google Scholar
Roux, P. L. Le, and Speakman, J. B., 1957. “The Plasticity of Wool. III. The Physical and Chemical Causes of Variation”, Text Res. (J.), 27, I–7.Google Scholar
Rudall, K. M., 1956. “The Keratinization of Horn”, Proc. Int. Wool Text. Res. Conf. Aust., 1955, F. 176–185.Google Scholar
Ryder, M. L., 1955. “Studies on the Nutrition of Wool Follicles in Sheep: The Anatomy of the General Blood Supply to the Skin”, J. Agric. Sci., 45, 311–326.Google Scholar
Ryder, M. L., 1956 a. “The Blood Supply to the Wool Follicle”, Proc. Int. Wool Text. Res. Conf. Aust., 1955, F. 63–91.Google Scholar
Ryder, M. L., 1956 b. “Studies on the Nutrition of Wool Follicles in Sheep”, Ph.D. Thesis, University of Leeds.Google Scholar
Ryder, M. L., 1956 c. “Use of Radioisotopes in the Study of Wool Growth and Fibre Composition”, Nature, Lond., 178, 1409–1410.CrossRefGoogle Scholar
Ryder, M. L., 1958 a. “Studies of Sulphur, Phosphorus and Some Metals in Skin Follicles” J. Histochem. Cytochem. [In the press.]Google Scholar
Ryder, M. L., 1958 b. “Some Observations on the Glycogen of the Wool Follicle” Quart. J. Micr. Sci., 99, 221–228.Google Scholar
Scott, E. J.van, and Flesch, P., 1954. “Sulphydryl and Disulphide in Keratinization”, Science, 119, 70–71.Google Scholar
Sylven, B., 1950. “The Qualitative Distribution of Metachromatic Polysaccharides Material During Hair Growth”, Exp. Cell Res., 1, 582–589.Google Scholar
Walser, M., Reid, A. F., and Seldin, D. W., 1953. “A Method of Counting Radiosulphur in Liquid Samples and its Application to the Determination of 35s Excretion following injection of 35so4“, Arch. Biochem. Biophys., 45, 91–96.Google Scholar
Wilcoxan, F., 1947. “Probability Tables for Individual Comparisons by Ranking Methods”, Biometrics, 3, 119.Google Scholar
Wildman, A. B., 1954. The Microscopy of Animal Textile Fibres. Wool Industries Research Association, Leeds.Google Scholar