Hostname: page-component-cd9895bd7-hc48f Total loading time: 0 Render date: 2024-12-25T06:28:43.559Z Has data issue: false hasContentIssue false
  • English
  • Français

The physiology of nitrate dissimilatory bacteria from the Tay Estuary

Published online by Cambridge University Press:  05 December 2011

R. A. Herbert ,
G. M. Dunn  and
C. M. Brown
R. A. Herbert
Affiliation:
Department of Biological Sciences, University of Dundee
G. M. Dunn
Affiliation:
Department of Biological Sciences, University of Dundee
C. M. Brown
Affiliation:
Department of Biological Sciences, University of Dundee
Get access

Synopsis

The physiology of three nitrate respiring bacteria, identified as Klebsiella strain K312, Pseudomonas strain P388 and Vibrio strain V25, obtained from Kingoodie Bay sediments in the River Tay by enrichment techniques, have been studied in continuous culture. In glycerol limited anaerobic cultures Klebsiella K312 produced NO2 as the primary product of nitrate reduction whereas under N-limited conditions NH4+ was excreted into the culture medium. Nitrate reduction in this organism was accompanied by the synthesis of a paniculate nitrate reductase (NR) whilst under N-limitation a soluble nitrite reductase (NiR) was also produced. In contrast, Pseudomonas P388 dissimilates NO3 directly to gaseous products under anaerobic conditions and was associated with the synthesis of a particulate NR and under N-limited conditions a NiR. Nitrite never accumulated as an intermediate. Vibrio V25 would only reduce NO3 as far as NO2 and was therefore restricted in its use of NO3 under anaerobic conditions. Klebsiella K312 was the most versatile of the three organisms studied and since Klebsiella spp were found in significant numbers in the Kingoodie sediments they may account in part for the high interstitial NH4+ levels found there.

Type
Research Article
Information
Proceedings of the Royal Society of Edinburgh, Section B: Biological Sciences , Volume 78 , Issue 3-4 , 1980 , pp. s79 - s87
Copyright
Copyright © Royal Society of Edinburgh 1980

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Brown, C. M., Ellwood, D. C. and Hunter, J. R., 1977. Growth of bacteria at surfaces: influence of nutrient limitation. F.E.M.S. Microbiol. Lett., 1, 163–166.CrossRefGoogle Scholar
Brown, C. M., Macdonald-Brown, D. S. and Stanley, S. O., 1972. Inorganic nitrogen metabolism in marine bacteria: nitrogen assimilation in some marine pseudomonads. J. Mar. Biol. Ass. U.K., 52, 793–804.CrossRefGoogle Scholar
Cole, J. A., 1968. Cytochrome C552 and nitrite reduction in E. coli. Biochim. Biophys. Acta, 162, 356–368.Google Scholar
Cole, J. A., Coleman, K. J., Compton, B. E., Kavnagh, B. M. and Keevil, C. M., 1974. Nitrite and ammonia assimilation by anaerobic continuous cultures of E. coli. J. Gen. Microbiol., 85, 11–22.Google Scholar
Cowan, S. T. and Steel, K. J., 1974. Identification of medical bacteria. Cambridge Univ. Press.Google Scholar
Downey, R. J. and Nuner, J. H., 1967. Induction of nitrate reductase under conditions of nitrogen depletion. Life Sci., 6, 855–861.CrossRefGoogle ScholarPubMed
Dunn, G. M., Herbert, R. A. and Brown, C. M., 1977. Physiology of denitrifying bacteria from tidal mudflats in the River Tay. In McLusky, D. S. and Berry, A. J. (eds) Proceedings of the 12th European Symposium on Marine Biology, Stirling, Scotland, 135—140. Oxford: Pergamon.Google Scholar
Dunn, G. M., Herbert, R. A. and Brown, C. M., 1978. The anaerobic production of ammonia from nitrate and nitrite by Klebsiella spp. Proc. Soc. Gen. Microbiol., 5(4), 102–103.Google Scholar
Dunn, G. M., Wardell, J. N., Herbert, R. A. and Brown, C. M., 1980. Enrichment, enumeration and characterization of nitrate-reducing bacteria present in sediments of the River Tay Estuary. Proc. Roy. Soc. Edinb., 78B, s47–s56.Google Scholar
Evans, C. G. T., Herbert, D. and Tempest, D. W., 1970. The continuous culture of microorganisms (2) Construction of a chemostat. In Norris, J. R. and Ribbons, D. W. (eds) Methods of Microbiology, vol.2. London: Academic.Google Scholar
Fewson, C. A. and Nicholas, D. J. D., 1961. Nitrate reductase from Ps. aeruginosa. Biochim. Biophys. Acta, 49, 335–349.CrossRefGoogle Scholar
Forget, P., 1971. Les nitrate-reductases bacteriennes. Solubilisation, purification et proprietes de l'enzyme A de Micrococcus denitrificans. Eur. J. Biochem., 18, 442—450.CrossRefGoogle Scholar
Greenberg, E. P. and Becker, G. E., 1972. A comparison of denitrification by three strains of Ps. fluorescens. Bad. Proc, 72, 175.Google Scholar
Herbert, R. A., 1975. Heterotrophic nitrogen fixation in shallow estuarine sediments. J. Exp. Mar. Biol. Ecol., 18, 215–225.CrossRefGoogle Scholar
Hollis, D. G., Wiggins, G. L. and Weaver, R. E., 1972. An unclassified Gram negative rod isolated from the pharynx on Thayer-Martin medium. Appl. Microbiol., 24, 772–777.CrossRefGoogle ScholarPubMed
Inderlied, C. B. and Delwiche, E. A., 1973. Nitrate reduction and growth of Veillonellaalcalscens. J. Bad., 114, 1206–1212.Google Scholar
Ishaque, M. and Aleem, M. I. H., 1972. Intermediates of denitrification in Thiobacillus denitrificans. Bad. Proc, 72, 175.Google Scholar
Iwasaki, H. and Matsubara, T., 1971. A nitrite reductase from A. cycloclastes. J. Biochem., 71, 645–652.Google Scholar
Kemp, J. D. and Atkinson, D. E., 1966. Nitrite reductase of E. coli specific for reduced nicotinamide adenine dinucleotide. J. Bact., 92, 628–638.CrossRefGoogle ScholarPubMed
Lowe, R. H. and Evans, H. J., 1964. Preparation and some properties of a soluble nitrate reductase from Rhizobium japonicum. Biochim. Biophys. Ada, 85, 377–389.Google ScholarPubMed
Matsubara, T. and Iwasaki, H., 1971. Enzymatic steps of dissimilatory nitrite reduction in Alcaligenes faecalis. J. Biochem., 69, 991–1001.Google Scholar
Payne, W. J., 1973. Reduction of nitrogenous oxides by microorganisms. Bad. Rev., 37, 409–452.Google ScholarPubMed
Payne, W. J., Riley, P. S. and Cox, C. D., 1971. Separate nitrite, nitric oxide and nitrous oxide reducing fractions from Ps. perfeclomarinus. J. Bad., 106, 356–361.Google Scholar
Pichinoty, F., 1964. A propos de nitrate-reductases d'une bacteria denitrificante. Biochim. Biophys. Acta, 89,378–381.Google Scholar
Prakash, O. and Sadana, T.C., 1973. Metabolism of nitrate in Achromobacter fischeri. Can. J. Microbiol., 19, 15–25.CrossRefGoogle Scholar
Radcliffe, B. C. and Nicholas, D. J. D., 1968.Some properties of a nitrate reductase from Pseudomonas denitrificans. Biochim. Biophys. Acta, 153, 545–554.Google Scholar
Renner, E. D. and Becker, G. L., 1970. Production of nitric oxide and nitrous oxide during denitriflcation by Corynebacterium nephridii. Can. J. Microbiol., 9, 799–807.Google Scholar
Sperl, G. T. and Hoare, D. S., 1971. Denitrification with methanol. Selective enrichment for Hyphomicrobium species. J. Bact., 108, 733–736.CrossRefGoogle ScholarPubMed
Wolfe, J. and Barker, A. N., 1967. The genus Bacillus. In Gibbs, B. M. and Skinner, F. A. (eds) Society of Applied Bacteriology Technical Series 1B, 93–109. London: Academic.Google Scholar