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Glutathione reductase activity following re-aeration of hypoxically pretreated wheat roots

Published online by Cambridge University Press:  05 December 2011

G. Albrecht  and
E.-M. Wiedenroth
G. Albrecht
Affiliation:
Humboldt Universität zu Berlin, FB Biologie, Institut für Allgemeine Botanik, Unter den Linden 6, D-10099 Berlin, Germany
E.-M. Wiedenroth
Affiliation:
Humboldt Universität zu Berlin, FB Biologie, Institut für Allgemeine Botanik, Unter den Linden 6, D-10099 Berlin, Germany
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Synopsis

During the first 2 h of oxygen re-exposure, the GSH level was almost constant, while the GSSG increased about 10-fold. This results in a decline of the GSH/GSSG ratio, which reflects oxidative stress induced by re-aeration following hypoxic pretreatment. Further evidence for this is an increase in lipid peroxidation measured as thiobarbituric acid-reactive material (TBA-rm) and the affected content of sulfydryl-groups in the root tissues.

In spite of the high level of reduced glutathione in the roots under hypoxia-inducing conditions, they contained a retarded glutathione reductase (GR) activity compared with aerobically grown roots. Re-aeration up to 2 h resulted in a further decrease in GR activity. Only at the end of the 16-h period of re-aeration the enzyme activity was able to recover, by overshooting slightly those values of the continuously aerated controls. This was accompanied by a restoring a high GSH/GSSG ratio and an enhanced level of GSH.

Type
Short Communications
Information
Proceedings of the Royal Society of Edinburgh, Section B: Biological Sciences , Volume 102: Oxygen and Environmental Stress in Plants , 1994 , pp. 413 - 417
Copyright
Copyright © Royal Society of Edinburgh 1994

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