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Effect of ageing on oxygen radical generation by soybean seeds

Published online by Cambridge University Press:  05 December 2011

Marcela Simontacchi  and
Susana Puntarulo
Marcela Simontacchi
Affiliation:
Fisicoquímica, Facultad de Farmacia y Bioquímica, Junín 956, 1113 Buenos Aires, Argentina
Susana Puntarulo
Affiliation:
Fisicoquimica, Facultad de Farmacia y Bioquimica, Junín 956, 1113 Buenos Aires, Argentina
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Synopsis

Rates of leakage of protein and electrolytes were measured in soybean embryonic axes from soybean seeds stored for 8–42 months. A significant increase in the content of proteins and electrolytes was determined in the external medium where aged embryonic axes were incubated. Lipid peroxidation was evaluated by the measurement of thiobarbituric acid-reactive substances (TBARS) in isolated axes from aged seeds. No significant differences were detected either in the content in vivo nor in the rate of generation in vitro. To assess in vivo oxidative condition of the axes, an assay employing 2′,7′-dichlorofluorescein (DCFH-DA) oxidation was used. Embryonic axes from seeds stored for 8 and 42 months showed 1.1±0.1 and 4.1±0.1 fluorescence units per min per axes, respectively, after 24 h of imbibition. This significant increase in fluorescence was measured at all the tested incubation times. Antioxidant defences, evaluated as chemiluminescence induced by t-butyl hydroperoxide and α-tocopherol content, showed a significant increase with storage time. These data suggest that the damage due to enhanced peroxidative events are limited by the increased amount of potent lipid soluble antioxidant.

Type
Short Communications
Information
Proceedings of the Royal Society of Edinburgh, Section B: Biological Sciences , Volume 102: Oxygen and Environmental Stress in Plants , 1994 , pp. 295 - 302
Copyright
Copyright © Royal Society of Edinburgh 1994

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