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Monoclonal antibodies in clinical chemistry

Published online by Cambridge University Press:  05 December 2011

W. M. Hunter
W. M. Hunter
Affiliation:
M.R.C. Immunoassay Team, 2 Forrest Road, Edinburgh EH1 2QW
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Synopsis

Immunoassays have markedly enlarged the armamentarium of the clinical chemist during the last twenty years. Radioimmunoassays and their variants, in particular, now provide evidence which is central to diagnosis and patient management in endocrinology and certain other disorders. Currently used methods employ labelled antigens in the radioimmunoassay (RIA) method first described for insulin by Yalow and Berson. The advent of monoclonal antibodies seems to remove the one major barrier to the use of methods based upon labelled antibodies–the immunoradiometric (IRMA) method as proposed by Miles and Hales. These procedures offer advantages of speed, sensitivity, working range, specificity and robustness which strongly suggest that they will replace RIA for the microdetermination of specific proteins. Nevertheless, their specificity is based upon the recognition of a single epitope on the antigen and thorough testing on varied patient material will be required before they can safely be adopted for routine use.

Type
Research Article
Information
Proceedings of the Royal Society of Edinburgh, Section B: Biological Sciences , Volume 81 , Issue 4: Clinical applications of monoclonal antibodies , 1982 , pp. 293 - 300
Copyright
Copyright © Royal Society of Edinburgh 1982

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References

Addison, G. M. and Hales, C. N. 1971. In Radioimmunoassay Methods. European Workshop, Edinburgh, ed. Kirkham, K. E. and Hunter, W. M., pp. 481–487. Edinburgh: Churchill Livingstone.Google Scholar
Albano, J. D. M., Ekins, R. P.. Maritz, G. and Turner, R. C. 1972. A sensitive, precise radioimmunoassay of serum insulin relying on charcoal separation of bound and free hormone moieties. Ada Endocr. 70, 487–509.Google ScholarPubMed
Bacon, R. R. A. and Hunter, W. M. 1981. Unpublished.Google Scholar
Ganguli, P. C. and Hunter, W. M. 1972. Radio-immunoassay of gastrin in human plasma. J. Phvsiol. 220, 499–510.Google ScholarPubMed
van Heyningen, V., Barron, L., Brock, D., Lawrie, S. and Crichton, D. 1982. Monoclonal antibodies to human alpha-foetoprotein: I. Behaviour in radioimmunoassay. J. Immun. Metli. (in press).Google Scholar
Hunter, W. M. 1976. Growth hormone. In Hormone Assays and their Clinical Application, 4th edn, ed. Loraine, J. A. and Bell, E. Trevor, pp. 221–284. Edinburgh: Churchill Livingstone.Google Scholar
Hunter, W. M. and Bennie, J. G. 1979. Reduction of non-specific serum responses in human pituitary gonadotrophin radioimmunoassays. J. Endocr. 80, 59–68.CrossRefGoogle ScholarPubMed
Hunter, W. M. and Budd, P. S. 1980. Circulating antibodies to ovine and bovine immunoglobulin in healthy subjects: a hazard for immunoassays. Lancet II, 1136.CrossRefGoogle Scholar
Hunter, W. M. and Budd, P. S. 1981. Immunoradiometric versus radioimmunoassay: a comparison using alpha-fetoprotein as the model analyte. J. Immun. Meth. 45, 255–273.CrossRefGoogle ScholarPubMed
Hunter, W. M. and McKenzie, I. 1979. Quality control of radioimmunoassays for proteins: the first two and a half years of a national scheme for serum growth hormone measurements. Ann. Clin. Biochem. 16, 131–146.CrossRefGoogle Scholar
Hunter, W. M., McKenzie, I. and Bacon, R. R. A. 1981. External quality assessment schemes for immunoassays. In Immunoassays for the Eighties, ed. Voller, A., Bartlett, A. and Bidwell, D., pp. 155–168. Lancaster: MTP Press.Google Scholar
Hunter, W. M., van Look, P. and Baird, D. T. 1979. Clinical application of refined LH and FSH assays to hyposecretory states. In Radioimmunology, ed. Bizollon, Ch. A., pp. 231–236. Amsterdam: Elsevier/North Holland Biomedical Press.Google Scholar
Hunter, W. M., Bennie, J. G., Brock, D. J. H. and van Heyningen, V. 1982. Monoclonal antibodies for use in an immunoradiometric assay for x-fetoprotein. J. Immun. Meth. (in press).CrossRefGoogle Scholar
Irvine, W. J., Toft, A. D., Hunter, W. M. and Kirkah, K. E. 1973. An assessment of plasma TSH radioimmunoassay and of the TSH stimulation test in the diagnosis of 100 consecutive patients with suspected hypothyroidism. Clin. Endocr. 2, 135–139.CrossRefGoogle ScholarPubMed
Knopf, R. F., Conn, J. W., Floyd, J. C. Jr, Fajans, S. S., Rull, J. A., Guntsche, E. M. and Thiffault, C. A. 1966. The normal endocrine response to ingestion of protein and infusions of amino acids. Sequential secretion of insulin and growth hormone. Trans Ass. Am. Physns 79, 312.Google ScholarPubMed
Lind, T., de Groot, H. A. van, C., Brown, G. and Cheyne, G. A. 1972. Observations on blood glucose and insulin determinations. Br. Med. J. 3, 320–323.CrossRefGoogle ScholarPubMed
Loraine, John A. and Trevor Bell, E. 1976. Hormone Assays and their Clinical Application, 4th edn. Edinburgh: Churchill Livingstone.Google Scholar
Miles, L. E. M. 1975. Immunoradiometric assay – new developments in theory and practice. Ric. Clin. Lab. 5, 695–703.Google Scholar
Miles, L. E. M. and Hales, C. N. 1968. Labelled antibodies and immunological assay systems. Nature, Lond. 219, 186–189.CrossRefGoogle ScholarPubMed
Secher, D. and Burke, D. C. 1980. A monoclonal antibody for large-scale purification of human leukocyte interferon. Nature, Lond. 285, 446–450.CrossRefGoogle ScholarPubMed
Wide, Leif. 1971. Solid phase antigen-antibody systems. In Radioimmunoassay Methods. European Workshop, Edinburgh, ed. Kirkham, K. E. and Hunter, W. M., pp. 405–412. Edinburgh: Churchill Livingstone.Google Scholar
Yalow, R S. and Berson, S. A. 1960. Immunoassay of endogenous plasma insulin in man. J. Clin. Invest. 39, 1157–1175.CrossRefGoogle ScholarPubMed
Yalow, R. S. and Berson, S. A. 1961. Immunological specificity of human insulin: application to immunoassay of insulin. J. Clin. Invest. 40, 2190–2208.CrossRefGoogle ScholarPubMed