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Free radical processes induced by desiccation in germinating maize. The relationship with respiration and loss of desiccation tolerance

Published online by Cambridge University Press:  05 December 2011

O. Leprince ,
G. A. F. Hendry  and
N. M. Atherton
O. Leprince
Affiliation:
USDA-ARS National Seed Storage Laboratory, 1111 S. Mason Street, Fort Collins, CO 80521, USA
G. A. F. Hendry
Affiliation:
Unit of Comparative Plant Ecology, Department of Animal and Plant Sciences, University of Sheffield, Sheffield S10 2TN, UK
N. M. Atherton
Affiliation:
Department of Chemistry, University of Sheffield, Sheffield S10 2TN, UK
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Synopsis

Using germination of maize as a model, desiccation-induced free radical processes were studied with the object of understanding desiccation tolerance. Several significant elements of damage were observed in desiccated material associated with development of desiccation intolerance: increased lipid peroxidation, phospholipid de-esterification, build-up of a stable free radical, supression or repression of respiratory enzymes from complex I, II and IV. An EPR (electron paramagnetic resonance) response was also detected in isolated mitochondria following in vitro desiccation. The loss of desiccation tolerance appeared to be dependent on oxygen concentration. Two highly significant correlations were independently found between respiration rates and production of a stable free radical detected by EPR. These data suggest that respiration is an important factor in the loss of desiccation tolerance. We present a model suggesting that activated oxygen formation during desiccation originates in the disruption of the mitochondrial electron transport chain with increasing leakage to oxygen so generating irreversible and lethal peroxidative damage, leading to the development of desiccation intolerance.

Type
Research Article
Information
Proceedings of the Royal Society of Edinburgh, Section B: Biological Sciences , Volume 102: Oxygen and Environmental Stress in Plants , 1994 , pp. 211 - 218
Copyright
Copyright © Royal Society of Edinburgh 1994

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