The presence of ices along the line of sight of high mass star forming regions is clearly evidenced by infrared observations. In dense molecular clouds icy grain mantles form after direct freeze out of gas phase species and after surface reactions of atoms and radicals on grains. Thus chemical composition of icy mantles differs from that of the gas phase. Due to the presence of the protostar and of cosmic radiation, icy mantles suffer from ion bombardment, UV photolysis and thermal annealing. Most of our knowledge on the physical and chemical properties of ices is based on the comparison between observations and laboratory experiments performed at low temperature (10-80 K). Experimental results show that after ion irradiation and UV photolysis the chemical composition and the structure of the sample is modified. Both more volatile and less volatile species are formed and if a C-bearing species is present in the original sample a refractory residue is left over after warm-up to room temperature. After thermal annealing, segregation, crystallization, and sublimation take place. Thus molecular species are released to the gas phase which could be enriched by species formed in the solid phase. Here I will discuss some recent laboratory experiments relevant to our knowledge of the physico-chemical properties of ices in star forming regions.