We discuss the expected properties of the first stellar generations in the Universe. We find that it is possible to discern truly primordial populations from the next generation of stars by measuring the metallicity of high-z star forming objects through the detection and the study of the emission line spectrum from HII regions associated to primordial massive stars. Moreover, the intensity of HeII emission lines can be used as a diagnostic to reveal the presence of massive stars and determine their importance relative to lower mass stars. Thanks to its very low background, the future James Webb Space Telescope (JWST) will be able to image and study first-light sources at very high redshifts. On the other hand, JWST's relatively small collecting area limits its capability in obtaining spectra of z$\,{\sim}\,$10–15 first-light sources to either the bright end of their luminosity function or to strongly lensed sources. With a suitable investment of observing time JWST will be able to detect individual Population III supernovae, thus identifying the very first stars that formed in the Universe.