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Production of polyclonal antibody for norfloxacin detection using immunoassays (ELISA)

Published online by Cambridge University Press:  20 November 2017

S.P. Gobbo ,
K.M.R. Duarte ,
P.A. Bricarello ,
S.M.G. Fedrizzi ,
F.C.A. Tavares  and
C. F. Meirelles
S.P. Gobbo*
Affiliation:
Laboratory of Animal Nutrition – Centre for Nuclear Energy in Agriculture (CENA/USP), CP 96, CEP 13400-970, Piracicaba, SP, Brazil.
K.M.R. Duarte
Affiliation:
Laboratory of Yeast Genetics – (ESALQ/USP)
P.A. Bricarello
Affiliation:
Laboratory of Animal Nutrition – Centre for Nuclear Energy in Agriculture (CENA/USP), CP 96, CEP 13400-970, Piracicaba, SP, Brazil.
S.M.G. Fedrizzi
Affiliation:
Laboratory of Animal Nutrition – Centre for Nuclear Energy in Agriculture (CENA/USP), CP 96, CEP 13400-970, Piracicaba, SP, Brazil.
F.C.A. Tavares
Affiliation:
Laboratory of Yeast Genetics – (ESALQ/USP)
C. F. Meirelles
Affiliation:
Laboratory of Animal Nutrition – Centre for Nuclear Energy in Agriculture (CENA/USP), CP 96, CEP 13400-970, Piracicaba, SP, Brazil.
*
E-mail: [email protected]
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Extract

The indiscriminate use of antibiotics to increase animal production may lead to residues in animal products, what justifies the monitoring of those chemicals (Heitzman, 1996). The intensification of livestock production in the world has been observed with an increasing use of drugs and growing promoter substances used as animal treatment or disease prevention. Several analytic techniques have been used to detect antibiotics residues of animal products such as fluorometry, liquid-gas chromatography, high performance liquid chromatography and immunological methods. The use of ELISA (“enzyme-linked-immunosorbent assays”) combined to specific antibodies has been employed on routine clinic diagnosis, identifying and quantifying species and substances. In this study, the objective was the production of polyclonal antibody to detect the norfloxacin antibiotic by indirect ELISA method.

Type
Poster Presentations
Information
Proceedings of the British Society of Animal Science , Volume 2004 , 2004 , pp. 106
Copyright
Copyright © The British Society of Animal Science 2004

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References

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