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Published online by Cambridge University Press: 23 November 2017
Ruminal fermentation produces methane (CH4), which represents a loss of feed energy and a significant source of greenhouse gas. Researches have been searching for ways of inhibiting ruminal CH4 yield and promising results have been achieved in in vitro assays (Hu et al., 2005). The in vitro gas production technique can predict the in vivo enteric CH4 production from ruminants. Our research focused on the effect of 2-Bromoethanesulfonic acid (BES) on gas and CH4 production and microbial variation of Lucerne and Tifton-85 hays fermented in vitro.