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Isomerisation of trans-10 cis-12 conjugated linoleic acid during acidic methylation
Published online by Cambridge University Press: 22 November 2017
Extract
Conjugated linoleic acid (CLA) has been associated with numerous health benefits in animal models including modulation of immune response and fat deposition (Lock & Bauman, 2004). The most predominant CLA in ruminant products (meat and milk) is cis-9 trans-11 with traces of other isomers such as trans-10 cis-12 which can be increased according to dietary regimes such as increasing the proportion of concentrate in the diet (Sackmann et al. 2003). More recent findings into the health benefits of CLA in humans have found the trans-10 cis-12 isomer to be more potent than cis-9 trans-11 (Tholstrup et al. 2008). Whilst it has been reported that acidic methylation during analysis of milk fat results in isomerisation of cis-9 trans-11 (Lee & Tweed, 2008) little information is available on the effect of different methylation regimes on the increasingly important trans-10 cis-12 isomer. This study investigated the isomerisation of esterified and un-esterified trans-10 cis-12 CLA during three methylation regimes.
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