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In vitro rumen fungi quantification in medium containing sodium hydroxide or formaldehyde treated sunflower meal using quantitative competitive PCR assay

Published online by Cambridge University Press:  22 November 2017

T Mohammadabadi ,
M Danesh Mesgaran ,
A Heravi Moussavi ,
M R Nassiry ,
M H Sekhavati ,
A Fani Maleki ,
M Chaji  and
A R Alshowkany
T Mohammadabadi*
Affiliation:
Department of Animal Science, Ramin Agriculture and Natural Resource University, Molasani, Ahvaz, Islamic Republic of Iran
M Danesh Mesgaran
Affiliation:
Department of Animal Science, Exellence Centre for Animal Sicence, Ferdowsi University of Mashhad, PO Box, 91775-1163, Mashhad, Islamic Republic of Iran
A Heravi Moussavi
Affiliation:
Department of Animal Science, Exellence Centre for Animal Sicence, Ferdowsi University of Mashhad, PO Box, 91775-1163, Mashhad, Islamic Republic of Iran
M R Nassiry
Affiliation:
Department of Animal Science, Exellence Centre for Animal Sicence, Ferdowsi University of Mashhad, PO Box, 91775-1163, Mashhad, Islamic Republic of Iran
M H Sekhavati
Affiliation:
Department of Animal Science, Exellence Centre for Animal Sicence, Ferdowsi University of Mashhad, PO Box, 91775-1163, Mashhad, Islamic Republic of Iran
A Fani Maleki
Affiliation:
Department of Animal Science, Exellence Centre for Animal Sicence, Ferdowsi University of Mashhad, PO Box, 91775-1163, Mashhad, Islamic Republic of Iran
M Chaji
Affiliation:
Department of Animal Science, Ramin Agriculture and Natural Resource University, Molasani, Ahvaz, Islamic Republic of Iran
A R Alshowkany
Affiliation:
Department of Animal Science, Exellence Centre for Animal Sicence, Ferdowsi University of Mashhad, PO Box, 91775-1163, Mashhad, Islamic Republic of Iran
*
Email: [email protected]
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Extract

The rumen anaerobic fungi are primary colonizers of fibrous plant materials that degrade lignin-containing plant cell walls. With the advancement of molecular enumeration methods, in particular 18S rDNA gene probing methods, researchers were able to monitor fungal species within the rumen (Stahl et al., 1998). Quantitative competitive PCR (QCPCR) techniques play an important role in nucleic acid quantification because of their significant lower cost of equipment and consumables (Franz et al., 2001). The aim of this study was to use the QC-PCR assay to evaluate the relative quantitative comparison of anaerobic fungi population in medium containing sunflower meal, either untreated or treated with formaldehyde (3 and 6 g/kg DM) or sodium hydroxide (40 g/kg DM).

Type
Theatre Presentations
Information
Proceedings of the British Society of Animal Science , Volume 2009 , April 2009 , pp. 175
Copyright
Copyright © The British Society of Animal Science 2009

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References

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