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In vitro embryo production from bovine oocyte donors aspirated at different frequencies or following treatment with FSH

Published online by Cambridge University Press:  05 November 2021

K.L. Goodhand ,
R.G. Watt ,
M.E. Staines ,
L.C. Higgins ,
P.J. Broadbent  and
J.S.M. Hutchinson
K.L. Goodhand
Affiliation:
Scottish Agricultural College, 581 King Street, Aberdeen, AB9 1UD University of Aberdeen, 581 King Street, Aberdeen, AB9 1UD
R.G. Watt
Affiliation:
Scottish Agricultural College, 581 King Street, Aberdeen, AB9 1UD
M.E. Staines
Affiliation:
Scottish Agricultural College, 581 King Street, Aberdeen, AB9 1UD
L.C. Higgins
Affiliation:
Scottish Agricultural College, 581 King Street, Aberdeen, AB9 1UD
P.J. Broadbent
Affiliation:
Scottish Agricultural College, 581 King Street, Aberdeen, AB9 1UD
J.S.M. Hutchinson
Affiliation:
University of Aberdeen, 581 King Street, Aberdeen, AB9 1UD
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Extract

The combination of in vivo recovery of oocytes using transvaginal ultrasound guided aspiration and subsequent in vitro embryo production can be used to increase the rate of genetic change for efficiency of beef production by increasing selection intensity and reducing generation interval. The total number of oocytes recovered by aspiration and embryos produced is directly proportional to the number of aspiration sessions whether recovery takes place once or twice weekly. Pre-treatment of oocyte donors with FSH has been shown to improve the number of follicles available for aspiration but effects on embryo production have been conflicting (Bungartz et al., 1995; Goodhand et al., in press). The objective of this experiment was to compare the effect on embryo production of frequency of follicular aspiration and pre-treatment of donor cattle with FSH.

Type
Beef & Sheep Physiology
Information
Proceedings of the British Society of Animal Science , Volume 1996 , March 1996 , pp. 68
Copyright
Copyright © The British Society of Animal Science 1996

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References

Bungartz, L., Lucas-Hahn, A., Rath, D. and Niemann, H. 1995. Collection of oocytes from cattle via follicular aspiration guided by ultrasound with or without gonadotrophin pretreatment and in different reproductive stages. Theriogenology, 43: 667–675.10.1016/0093-691X(94)00072-3CrossRefGoogle ScholarPubMed
Goodhand, K.L., Broadbent, P.J., Hutchinson, J.S.M., Watt, R.G., Staines, M.E. and Higgins, L.C. 1996. In-vivo oocyte recovery and in-vitro embryo production in cattle pre-treated with FSH, progestogen and estradiol. Theriogenology, In press.10.1016/0093-691X(96)84828-XCrossRefGoogle Scholar