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Fibrolytic enzyme mixtures as an alternative to rumen fluid for assessing feed degradation kinetics in vitro

Published online by Cambridge University Press:  20 November 2017

D. Colombatto ,
F. L. Mould ,
M. K. Bhat  and
E. Owen
D. Colombatto
Affiliation:
Department of Agriculture, The University of Reading, Earley Gate, PO Box 236, RG6 6AT, UK FQMS Dept, Institute of Food Research, Colney Lane, Norwich NR4 7UA, UK
F. L. Mould
Affiliation:
Department of Agriculture, The University of Reading, Earley Gate, PO Box 236, RG6 6AT, UK
M. K. Bhat
Affiliation:
FQMS Dept, Institute of Food Research, Colney Lane, Norwich NR4 7UA, UK
E. Owen
Affiliation:
Department of Agriculture, The University of Reading, Earley Gate, PO Box 236, RG6 6AT, UK
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Extract

Several techniques involving enzymes as alternatives to rumen fluid in in vitro studies have been proposed. However, high cost, ill-defined characterisation and high variation among enzyme preparations and batches have discouraged their use. In addition, most studies have aimed at determining dry matter degradability (DMD) at a fixed time, commonly 48 h. Consequently little information has been published concerning the DM degradation dynamics of forages incubated with enzymes. Therefore the objective of the present study was to compare the ability of a commercial enzyme mixture to describe the fermentation dynamics of two contrasting forages, using the ANKOM in vitro fermentation system (Daisy II, ANKOM Co, USA).

Type
Theatre Presentations
Information
Proceedings of the British Society of Animal Science , Volume 2000 , 2000 , pp. 27
Copyright
Copyright © The British Society of Animal Science 2000

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References

Colombatto, D., Bhat, M. K., Mould, F. L. and Owen, E. 1999. Characterisation and evaluation of a commercial enzyme for improving the nutritive value of ruminant feeds. Proceedings of the British Society of Animal Science, p 211.CrossRefGoogle Scholar
France, J; Dhanoa, M. S.; Theodorou, M. K.; Lister, S. J.; Davies, D. R. and Isac, D. 1993. A model to interpret gas accumulation profiles associated with in vitro degradation of ruminant feeds. Journal of Theoretical Biology, 163: 99–111.CrossRefGoogle Scholar