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Effect of monensin on in vitro semi-continuous rumen fermentation in the rumen simulation technique (Rusitec)

Published online by Cambridge University Press:  05 November 2021

E.D. Mackintosh ,
R.H. Phipps ,
J.D. Sutton  and
J. Wilkinson
E.D. Mackintosh
Affiliation:
Centre for Dairy Research, Department of Agriculture, The University of Reading, Arborfield Hall Farm, Arborfield, Reading RG2 9HX, United Kingdom
R.H. Phipps
Affiliation:
Centre for Dairy Research, Department of Agriculture, The University of Reading, Arborfield Hall Farm, Arborfield, Reading RG2 9HX, United Kingdom
J.D. Sutton
Affiliation:
Centre for Dairy Research, Department of Agriculture, The University of Reading, Arborfield Hall Farm, Arborfield, Reading RG2 9HX, United Kingdom
J. Wilkinson
Affiliation:
Elanco Animal Health, P.O. Box 708, Greenfield, Indiana 46140, United States of America
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Extract

Rusitec (Czerkawski and Breckenndge, 1977) lias been widely used to study factors which affect rumen fermentation such as monensin, monensin-propionate, abierixin, calcimycin and Aspergillus oryzae (Bogaert et al., 1990; Newbold et al., 1993). Monensin is a grain-positive ionophore which modifies rumen fermentation. The objective of the current study was to determine the effect of monensin on molar proportions of volatile fatty acid (VFA) produced from diets which varied in foragexoncentrate ratio.

Four Rusitec vessels (800 ml) were used in an extended Latin Square design with 2 blocks, 2 treatments and 3 periods, each lasting 14 days. To initiate each period, inocula was recovered from the same lactating Holstein-Friesian cow (∽650 kg). All vessels received 15 g DM/day of a complete diet which contained maize silage, grass silage, NaOH treated wheat grain and a protein supplement in three foragexoncentrate ratios; 25:75 (L), 50:50 (M) and 75:25 (H).

Type
Techniques
Information
Proceedings of the British Society of Animal Science , Volume 1996 , March 1996 , pp. 213
Copyright
Copyright © The British Society of Animal Science 1996

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References

Bogaert, C., Jouany, J.P. and Jeminet, G. 1990. Effects of the ionophore antibiotics monensin, monensin-propionate, abierixin and calcimycin on ruminal fermentations in vitro (Rusitec). Animal Feed Science and Technology 28: 183–197.10.1016/0377-8401(90)90152-XCrossRefGoogle Scholar
Czerkawski, J.W. and Breckenndge, G. 1977. Design and development of a long-term rumen simulation technique (Rusitec). British Journal of Nutrition 38: 371–384.10.1079/BJN19770102CrossRefGoogle Scholar
Newbold, C.J., McKain, N. and Wallace, R.J. 1993. Combined effects of Aspergillus oryzae extract and monensin on fermentation in the rumen simulation technique (Rusitec). Journal of Agricultural Science 121: 241–246.10.1017/S002185960007711XCrossRefGoogle Scholar
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