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Effect of incremental dietary fish oil inclusion on bacterial diversity in the rumen

Published online by Cambridge University Press:  23 November 2017

S.A. Huws ,
E.J. Kim ,
R. Sanderson ,
S. Muetzel ,
R.J. Wallace  and
N.D. Scollan
S.A. Huws*
Affiliation:
Institute of Grassland and Environmental Research (IGER), Aberystwyth, Wales, United Kingdom
E.J. Kim
Affiliation:
Institute of Grassland and Environmental Research (IGER), Aberystwyth, Wales, United Kingdom
R. Sanderson
Affiliation:
Institute of Grassland and Environmental Research (IGER), Aberystwyth, Wales, United Kingdom
S. Muetzel
Affiliation:
Rowett Research Institute (RRI), Aberdeen, Scotland, United Kingdom
R.J. Wallace
Affiliation:
Rowett Research Institute (RRI), Aberdeen, Scotland, United Kingdom
N.D. Scollan
Affiliation:
Institute of Grassland and Environmental Research (IGER), Aberystwyth, Wales, United Kingdom
*
Email:[email protected]
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Extract

In vitro experiments have revealed that members of the Butyrivibrio group can convert linoleic (C18:2 n-6) and linolenic (C18:3 n-3) acid to VA (C18:1 trans-11), with some being able to further convert VA to stearate (C18:0). Advances in molecular microbial technology mean that we are now able to quantify these bacteria using quantitative PCR (qPCR) as well as look at total eubacterial and Butyrivibrio–specific population changes using Denaturing Gradient Gel Electrophoresis (DGGE). The aims of this study were to assess the involvement of Butyrivibrio spp. in the biohydrogenation pathways in vivo as well as investigate whether other bacterial species may be involved.

Type
Theatre Presentations
Information
Proceedings of the British Society of Animal Science , Volume 2007 , April 2007 , pp. 22
Copyright
Copyright © The British Society of Animal Science 2007

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References

Huws, S. A., Lee, M. R. F., Muetzel, S., Wallace, R. J., & Scollan, N. D. (2006). Effect of forage type and level of fish oil inclusion on bacterial diversity in the rumen. RRI-INRA Gut Microbiology 2006: 5th Biennial Meeting, Aberdeen, 21-23 June.Google Scholar
Kim, E. J., Wood, J. D., Richardson, I., Huws, S. A., & Scollan, N. D. (2007). Effect of level of fish oil in the diet on flow of fatty acids to the small intestine in steers. British Society of Animal Science Annual Conference, Stockport, 2-4 April 2007.CrossRefGoogle Scholar
Paillard, D., McKain, N., Rincon, M. T., Shingfield, K. J., Givens, D. I., & Wallace, R. J. 2007. Quantification of ruminal stearic acid-producing bacteria by real-time PCR using a molecular beacon approach. Submitted to the Journal of Applied Microbiology.CrossRefGoogle Scholar