Human intakes of Very Long Chain n-3 Polyunsaturated Fatty Acids (VLC n-3 PUFAs) are sub-optimal and enrichment of animal-derived foods with C22:6 (DHA) provides a potential route to increased intakes. Chicken meat is popular and versatile, and has the potential to provide meaningful amounts of VLC n-3 PUFAs to the human diet if enriched with these fatty acids through supplementation of growing broiler diets. Marine oils are potential sources as they are known to be rich in DHA. The enrichment of edible poultry tissues with DHA, particularly breast muscle is well established. However, the effect of dietary supplementation of DHA, on the enrichment of other tissues of the chicken is not well known, therefore work to investigate the distribution of these fatty acids within the broiler chicken is valuable to further understand the distribution and enrichment process. The objective of the present study was to compare different levels of DHA in broiler diets in relation to its effect on DHA concentration in the major organ tissues of the broiler chicken.