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Published online by Cambridge University Press: 22 November 2017
Cellulolytic bacteria, such as Ruminococcus albus, R. flavefaciens and Fibrobacter succinogenes are major micro-organisms responsible for ruminal digestion of plant cell walls ingested by the animal due to their numerical predominance and metabolic diversity (Cheng et al., 1991). It has been proposed that sodium hydroxide might breakdown hemicelluloses, expose the cellulose to microbial attachment and improve digestibility (Chen et al., 2006). The objective of this experiment was to estimate the disappearance of dry matter (DM) and neutral detergent fibre (NDF) of sunflower meal (25 g fat/kg DM; SM) as untreated or treated with formaldehyde (3 g/kg DM) or sodium hydroxide (40 g/kg DM) using in vitro culture with isolated mixed rumen bacteria.