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Determination of total gas production and gas profile release from a bicarbonate buffer during addition of acetic acid to a carbonated-buffered medium

Published online by Cambridge University Press:  05 November 2021

R. M. Mauricio ,
D.M.S.S Vitti ,
F.L. Mould ,
E. Owen ,
M.S Dhanoa  and
M.K Theodorou
R. M. Mauricio
Affiliation:
Department of Agriculture, The University of Reading, Earley Gate, PO Box 236, Reading RG6 6AT
D.M.S.S Vitti
Affiliation:
Animal Science Section, CENA, Caixa Postal 96, CEP 13400-970, Sao Paulo, Brazil
F.L. Mould
Affiliation:
Department of Agriculture, The University of Reading, Earley Gate, PO Box 236, Reading RG6 6AT
E. Owen
Affiliation:
Department of Agriculture, The University of Reading, Earley Gate, PO Box 236, Reading RG6 6AT
M.S Dhanoa
Affiliation:
IGER, Plas Gogerddan, Aberystwyth, Ceredigion SY23 3EB
M.K Theodorou
Affiliation:
IGER, Plas Gogerddan, Aberystwyth, Ceredigion SY23 3EB
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Extract

The quantity of gas accumulated in the in vitro fermentation technique (e.g. Theodorou et al., 1994) results from gaseous end-products of substrate fermentation, lysis of rumen microorganisms and CO2 released when VFA are neutralised by the carbonate-buffered medium (Beuvink and Spoelstra, 1992). However the quantity of gas produced from this acid-buffer interaction is not directly related to substrate fermentation and therefore needs to be quantified if gas evolution from substrate fermentation is to be estimated. This study examined gas release following the addition of acetic acid to a bicarbonate buffered medium and used a Gompertz equation to describe both the rate and total volume produced.

Type
Programme
Information
Proceedings of the British Society of Animal Science , Volume 1998 , 1998 , pp. 58
Copyright
Copyright © The British Society of Animal Science 1998

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References

Beuvink, J.M.W. and Spoelstra, S.F., 1992. Interactions between substrate, fermentation end-products, buffering systems and gas production upon fermentation of different carbohydrates by mixed rumen microorganisms in vitro . Applied Microbiology 37:505–509.Google Scholar
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