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The detection of apoptosis and bcl2 in isolated secondary mohair follicles (growing and quiescent), cultured in vitro in the presence or absence of biotin

Published online by Cambridge University Press:  20 November 2017

A.M. Tahmasbi ,
H. Galbraith ,
J.R. Scaife  and
D. Finn
A.M. Tahmasbi
Affiliation:
Department of Agriculture, Aberdeen University, 581 King Street, Aberdeen, Scotland, AB24 5UA, UK
H. Galbraith
Affiliation:
Department of Agriculture, Aberdeen University, 581 King Street, Aberdeen, Scotland, AB24 5UA, UK
J.R. Scaife
Affiliation:
Department of Agriculture, Aberdeen University, 581 King Street, Aberdeen, Scotland, AB24 5UA, UK
D. Finn
Affiliation:
Department of Agriculture, Aberdeen University, 581 King Street, Aberdeen, Scotland, AB24 5UA, UK
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Extract

Apoptosis is a physiologically active process whereby cells die in a programmed manner (Stenn et al 1993). Growth of hair occurs as a consequence of proliferation and differentiation of the epidermal cells in the bulb of the hair follicles. Reduction of hair growth or loss of follicle viability may arise from reduction in proliferation, and also from apoptosis of epidermal hair shaft-forming cells. Increases in apoptosis have been associated with reduction in expression of the apoptosis inhibitor bcl2 (Lindner et al 1997). Previous studies (Tahmasbi et al 1998) have shown that biotin deficiency reduced the viability of mohair follicles and thymidine uptake in vitro. The aim of this work was to investigate the presence of apoptosis and bcl2 in follicles which maintained growth or were quiescent following culture in media unsupplemented or supplemented with biotin.

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Information
Proceedings of the British Society of Animal Science , Volume 1999 , 1999 , pp. 159
Copyright
Copyright © The British Society of Animal Science 1999

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References

Lindner, G., Botchkarev, V.A., Botchkarev, N.V., Ling, G., van der Veen, C. and Paus, R. 1997. Analysis of apoptosis during hair follicle regression (catagen). Am. J. Pathology 151:1601–1617.Google Scholar
Stenn, K.S., Lawrence, L., Veis, D., Korsmeyer, S. and Seiberg, M. 1994. Expression of the bcl2 protooncogene in the cycling adult mouse hair follicles. J. Invest. Dermatol. 103: 107–111.Google Scholar
Tahmasbi, A. M., Galbraith, H. and Scaife, J. R. 1998. Effect of biotin on growth, viability and apparent DNA synth in cultured secondary hair follicles of Angora goat. Proceedings BSAS Winter Meeting, Scarborough, P.138.Google Scholar