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A comparison of solvents for extraction of polyphenolic compounds in tree leaves

Published online by Cambridge University Press:  20 November 2017

CM Capetillo Leal ,
R. Reyes Ramírez ,
CA Sandoval-Castro  and
D. Camacho Morfin
CM Capetillo Leal
Affiliation:
Fac. Vet. Med. & Anim. Sci. – Univ. of Yucatan, México. Apdo. 4-116 Itzimna, Mérida, Yucatán, 97100, México
R. Reyes Ramírez
Affiliation:
Fac. Vet. Med. & Anim. Sci. – Univ. of Yucatan, México. Apdo. 4-116 Itzimna, Mérida, Yucatán, 97100, México
CA Sandoval-Castro
Affiliation:
Fac. Vet. Med. & Anim. Sci. – Univ. of Yucatan, México. Apdo. 4-116 Itzimna, Mérida, Yucatán, 97100, México
D. Camacho Morfin
Affiliation:
Fac. Vet. Med. & Anim. Sci. – Univ. of Yucatan, México. Apdo. 4-116 Itzimna, Mérida, Yucatán, 97100, México
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Extract

The occurrence of polyphenolics compounds in forage is of nutritional significance due to its protein binding capacity which can affect protein digestion (Woodward and Reed, 1989). They also have a role in controlling rates of decomposition and N mineralization of plant residues (Swift et al., 1979). The Prussian-blue assay (Price and Butler, 1977) is widely use for quantitative measurement of polyphenol content of plant materials due to the easiness it can be performed. Polyphenols are polar compound and literature report extractions with, methanol, ethanol or acetone as usual solvents. Although it is known that no single protocol is optimum for all samples. No comparison was found between solvents on a wide range of material to assess if results might be correlated. The objective of the present work was to compare the efficiency of four solvents for polyphenol extraction.

Type
Poster Presentations
Information
Proceedings of the British Society of Animal Science , Volume 2002 , 2002 , pp. 135
Copyright
Copyright © The British Society of Animal Science 2002

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References

Anderson, JM, Ingram, JSI 1989. Tropical soil biology and fertility. A handbook of methods. CAB Int. UK. Google Scholar
Bohn, PJ, Fales, SL 1991. Effect of two pre-treatments on phenolic acid yield in three forage species. J Sci. Food Agric. 54: 341–354.CrossRefGoogle Scholar
Hagerman, AE, Robbins, C 1987. Implications of soluble tannin-protein complexes for tannin analysis and plant defense mechanisms. J Chem. Ecol. 13: 1243–1253 CrossRefGoogle ScholarPubMed
MacTaggart, DL, Farwell, SO 1992. Analytical use of linear regresion. Part II: Statistical error in both variables. JAOAC Intnal. 75: 608–614 CrossRefGoogle Scholar
Price, ML, Butler, LG. 1977. Rapid visual estimation and spectrophotometric determination of tannin content of sorghum grain. J. Agric. Food Chem. 25: 1268–1273 CrossRefGoogle Scholar
Swift, MJ, Heal, OW, Anderson, JM. 1979 Studies in ecology. vol. 5. Blackwell Scientific Publications, Oxford, UK.Google Scholar
Woodward, A. Reed, J.D. 1989. The influence of polyphenolics on the nutritive value of browse: A summary of research conducted at ILCA. ILCA Bulletin 35. 2–11 Google Scholar