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A comparison of solvents for extraction of condensed tannins in tree leaves

Published online by Cambridge University Press:  20 November 2017

CM Capetillo Leal ,
R. Reyes Ramírez ,
CA Sandoval-Castro  and
D. Camacho Morfin
CM Capetillo Leal
Affiliation:
Fac. Vet. Med. & Anim. Sci. – Univ. of Yucatan, México. Apdo. 4-116 Itzimna, Mérida, Yucatán, 97100, México
R. Reyes Ramírez
Affiliation:
Fac. Vet. Med. & Anim. Sci. – Univ. of Yucatan, México. Apdo. 4-116 Itzimna, Mérida, Yucatán, 97100, México
CA Sandoval-Castro
Affiliation:
Fac. Vet. Med. & Anim. Sci. – Univ. of Yucatan, México. Apdo. 4-116 Itzimna, Mérida, Yucatán, 97100, México
D. Camacho Morfin
Affiliation:
Fac. Vet. Med. & Anim. Sci. – Univ. of Yucatan, México. Apdo. 4-116 Itzimna, Mérida, Yucatán, 97100, México
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Extract

The occurrence of tannins in plant leaves has a widely known ecological significance in terms of both nutritional constraint for animals and litter decay in the soil. However, there is no single protocol for extracting tannins for their subsequent quantification. The use of acetone has the advantage of inhibiting the polyphenol-protein interaction (Hagerman and Robbins, 1987), but becomes an disadvantage when assaying the protein precipitation capacity of the extract in biological assays (Hagerman and Butler, 1991). The objective of the present work was to compare tannin extracting agents and to assess the relationship arising between different solvents.

Type
Poster Presentations
Information
Proceedings of the British Society of Animal Science , Volume 2002 , 2002 , pp. 134
Copyright
Copyright © The British Society of Animal Science 2002

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References

Hagerman, AE, Butler, LG. 1991. Tannins and lignins. In. Herbivores: their interactions with secondary plant metabolites. Vol. I. Rosenthal, GA, Berembaum, MR. (eds.). Academic Press. New York. pp: 355–383 Google Scholar
Hagerman, AE, Robbins, C 1987. Implications of soluble tannin-protein complexes for tannin analysis and plant defense mechanisms. J Chem. Ecol. 13: 1243–1253 Google Scholar
Price, ML, Van Scoyoc, S, Butler, LG. 1978. A critical evaluation of the vanillin reaction assay for tannin in sorghum grain. J. Agric. Food Chem. 26: 1214–1218 Google Scholar