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Published online by Cambridge University Press: 23 November 2017
Dietary conjugated linoleic acids (CLA) offer significant health benefits for man, and ruminant products are the major dietary sources (Bauman et al., 2001). The synthesis of CLA in the ruminant animal occurs either directly in the rumen or in the tissue from trans -vaccenic acid (TVA), formed primarily as intermediate products of ruminal biohydrogenation of linoleic acid (C18:2) to stearic acid (C18:0). Within the rumen, the pattern of biohydrogenation and the products formed appear to differ between the particle rich and the liquid fractions of the rumen (Singh and Hawke, 1979), with biohydrogenation occurring primarily on small particles. The aim of this study was to investigate whether the pattern of CLA and TVA formation differs in these fractions.