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The use of fresh or thawed rumen fluid containing glycerol or particle associated microbes to estimate in vitro degradation of feeds

Published online by Cambridge University Press:  20 November 2017

R. Mohamed ,
A. S. Chaudhry  and
P. Rowlinson
R. Mohamed
Affiliation:
School of Agriculture, Food and Rural Development, University of Newcastle upon Tyne, NE1 7RU, UK
A. S. Chaudhry
Affiliation:
School of Agriculture, Food and Rural Development, University of Newcastle upon Tyne, NE1 7RU, UK
P. Rowlinson
Affiliation:
School of Agriculture, Food and Rural Development, University of Newcastle upon Tyne, NE1 7RU, UK
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Extract

Thawed rumen contents have been used to obtain strained rumen fluid (SRF) to estimate in vitro dry matter degradation (DMD) of feeds (Mohamed et al., 2002). However, thawed SRF (TSRF) gave lower DMD than fresh SRF (FSRF) which was partly attributed to reduced microbial activity in TRSF following storage at -20°C. This study examined the addition of glycerol (G) as cryopreservative and washing from particle associated microbes to SRF before its storage for later use as TSRF to estimate in vitro degradation of rapeseed meal (Rsd) and grass nut (Gnt).

Type
Feed Characterisation
Information
Proceedings of the British Society of Animal Science , Volume 2003 , 2003 , pp. 149
Copyright
Copyright © The British Society of Animal Science 2003

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References

Mohamed, R, Chaudhry, A. S, and Rowlinson, P. 2002. Fresh or frozen rumen contents as sources of inocula to estimate in vitro degradation of ruminant feeds. Proceeding of the British Society of Animal Science, York, p164.Google Scholar