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Rapid Assessment of Silage Fermentation Characteristics by Electrometric Titration

Published online by Cambridge University Press:  24 November 2017

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Affiliation:
The Scottish Agricultural College , AuchincruiveAyr, KA6 5HW
J.A. Rooke
Affiliation:
The Scottish Agricultural College581 King Street, AberdeenAB9 1UD
R.J. Dewhurst
Affiliation:
The Scottish Agricultural College , AuchincruiveAyr, KA6 5HW
C. Thomas
Affiliation:
The Scottish Agricultural College , AuchincruiveAyr, KA6 5HW
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Extract

Inaccurate prediction of silage intake is often the greatest source of error in ration formulation but improvements have been hindered by an inadequate description of the silage fermentation in advisory practice. Until recently, only pH and ammonia N have been routinely measured as indicators of silage fermentation characteristics. A new method, based on research in Finland (Moisio and Heikonen, 1989) has been developed and is now in use by SAC. This involves automated titration of juice squeezed from the silage to pH 2 (with HC1) followed by stepwise titration (with NaOH) to pH 12. Concentrations of juice constituents are predicted from the buffering capacities measured over segments of the titration curve (pH 2 to pH 12). Calibrations have been obtained empirically by the addition of known increments of standards to a range of silage juices. Proportions of variance (R2) of measurements made by reference methods accounted for by predictions from titrations for a validation set of 93 silages were 0.90, 0.78, 0.92, 0.82 and 0.82 for lactic acid, acetic+butyric acids (VFA), soluble N, sugar and ammonia N respectively.

Type
Intake and Milk Production
Copyright
Copyright © The British Society of Animal Production 1993

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References

Moisio, T and Heikonen, M (1989). Animal Feed Science and Technology. 22: 341353.CrossRefGoogle Scholar