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The use of a hyper-osmotic diluent for freezing ram semen

Published online by Cambridge University Press:  24 November 2017

Y.J. Ahangari ,
R.E.F. Axford  and
I. ApDewi
Y.J. Ahangari
Affiliation:
School of Agriculture & Forest Sciences, University of Wales, Bangor, Gwynedd. LL57 2UW
R.E.F. Axford
Affiliation:
School of Agriculture & Forest Sciences, University of Wales, Bangor, Gwynedd. LL57 2UW
I. ApDewi
Affiliation:
School of Agriculture & Forest Sciences, University of Wales, Bangor, Gwynedd. LL57 2UW
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Extract

When semen is frozen ice forms extracellularly, and water is drawn from the cells (Mazur, 1970). Rapid freezing reduces dehydration and causes intracellular ice to form more quickly (Mazur, 1980). Cells frozen rapidly in liquid nitrogen do not survive, suggesting that partial dehydration is necessary for spermatozoa to survive freezing (Fahy et aL, 1984). Watson (1979) suggested that non-penetrating sugars have protective effects on spermatozoa during freezing. The addition of a non-penetrating solute such as raffinose to semen will dehydrate the cells by its osmotic effect.

The object of this study was to investigate the effect of raffinose on cryoinjury of ram spermatozoa.

Experiment 1.Raffinose was added to Tris egg-yolk glycerol buffer (Evans and Maxwell (1987) to concentrations of 0mM, 33mM, 66mM, 99mM, 132mM, 165mM and 198mM raffinose and used to extend (2:1) pooled semen from Cambridge rams.

Factorial experiments with 3 replications were set up to test the effect of raffinose concentration, glycerol concentration, method of packaging of semen, pellet size and cooling rate of straws on the motility and survival of ram semen before and after freezing/thawing.

Type
Ruminant Reproduction
Information
Proceedings of the British Society of Animal Production (1972) , Volume 1993: Winter meeting , March 1993 , pp. 60
Copyright
Copyright © The British Society of Animal Production 1993

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References

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